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| 1 | CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing显示文摘 | Xie, Xianrong Ma, Xingliang Zhu, Qinlong Zeng, Dongchang Li, Gousi Liu, Yao-Guang | 2017 | Molecular Plant2017，10，9： | 67 |
| 2 | Suppression or knockout of SaF/SaM overcomes the Sa-mediated hybrid male sterility in rice显示文摘Hybrids between the indica and japonica subspecies of rice(Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used clustered regularly interspaced short palindromic repeats(CRISPR)/Cas9-based genome editing to knockout the SaF and SaM alleles, respectively, of an indica rice line to create hybrid-compatible lines. The resultant artificial neutral alleles did not affect pollen viability and other agricultural traits, but did break down the reproductive barrier in the hybrids. We found that some rice lines have natural neutral allele Sa-n, which was compatible with the typical japonica or indica Sa alleles in hybrids. Our results demonstrate that SaF and SaM are required for hybrid male sterility, but are not essential for pollen development. This study provides effective approaches for the generation of hybrid-compatible lines by knocking out the Sa locus or using the natural Sa-n allele to overcome hybrid male sterility in rice breeding. | Yongyao Xie Baixiao Niu Yunming Long Gousi Li Jintao Tang Yaling Zhang Ding Ren Yao-Guang Liu Letian Chen | 2017 | Journal of Integrative Plant Biology2017，59，9： | 11 |
| 3 | Genome-editing technologies: the gap between application and policy显示文摘Genome editing is a new technology for manipulating genomic DNA sequences at specific sites,and researchers worldwide are rapidly developing new variants of genomeediting applications that target mRNAs,viruses,chromatin structure,and other cellular processes. | Gousi Li Yao-Guang Liu Yuanling Chen | 2019 | Science China(Life Sciences)2019，62，11： | 9 |
| 4 | The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice显示文摘 | Yongyao Xie Yaling Zhang Jingluan Han Jikai Luo Gousi Li Jianle Huang Haibin WU Qingwei Tian Qinlong Zhu Yuanling Chen Yoji Kawano Yao-Guang Liu Letian Chen | 2018 | Molecular Plant2018，11，5： | 3 |
| 5 | Global characterization of OsPIP aquaporins reveals that the H_(2)O_(2)transporter OsPIP2;6 increases resistance to rice blast显示文摘Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress.To elucidate their roles in plant immunity to pathogen attack,we characterized the expression patterns,subcellular localizations,and H_(2)O_(2)-transport ability of 11 OsPIPs in rice(Oryza sativa),and identified OsPIP2;6 as necessary for rice disease resistance.OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H_(2)O_(2).Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae,indicating a positive function in plant immunity.OsPIP2;6 interacts with OsPIP2;2,which has been reported to increase rice resistance to pathogens via H_(2)O_(2)transport.Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction. | Gousi Li Jingluan Han Chen Yi Hao Luo Yuzhu Wang Fengpin Wang Xiaoyu Wang Letian Chen Yaling Zhang | 2024 | The Crop Journal2024，12，1： | 0 |
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