|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 2 | Opposite effects of nitrogen fertilization and plastic film mulching on crop N and P stoichiometry in a temperate agroecosystem显示文摘Aims Crop nitrogen(N)and phosphorus(P)stoichiometry can influence food nutritive quality and many ecosystem processes.However,how and why N and P stoichiometry respond to long-term agricul-tural management practices(e.g.N fertilization and film mulching)are not clearly understood.Methods We collected maize tissues(leaf,stem,root and seed)and soil sam-ples from a temperate cropland under 30-year continuous N fer-tilization and plastic film mulching treatments,measured their C,N and P concentrations(the proportion(%)relative to the sample mass),and used structural equation models to uncover the re-sponding mechanisms for crop N and P contents(the total amount(g/m2)in crop biomass).Important Findings Long-term N fertilization increased N concentrations in all crop tissues but sharply decreased P concentrations in vegetative tis-sues(leaf,stem and root),thereby reducing their C/N ratio and increasing C/P and N/P ratios.The drop in P concentration in vegetative tissues was due to the dilution effect by biomass in-crement and the priority of P supply for seed production.In con-trast,film mulching decreased N concentration but increased P concentrations in most crop tissues,thereby increasing C/N ratio and reducing C/P and N/P ratios.Film mulching increased crop P content by increasing soil temperature and moisture;whereas,mulching showed little effect on crop N content,because a posi-tive effects of soil temperature may have canceled out a negative effect by soil moisture.This indicated a decoupling of P and N uptake by crops under film mulching.In conclusion,N fertiliza-tion and plastic film mulching showed opposite effects of on crop N and P stoichiometry. | Fan Ding Shuangyi Li Xiao-Tao Lü Feike A.Dijkstra Sean Schaeffer Tingting An Jiubo Pei Liangjie Sun Jingkuan Wang | 2019 | Journal of Plant Ecology2019，12，4： | 8 |
| 3 | Cloning and expression analysis of the FvNCED3 gene and its promoter from ash(Fraxinus velutina)显示文摘The 9-cis-epoxycarotenoid dioxygenase(NCED)gene is rate-limiting in abscisic acid(ABA) biosynthesis.In this study, an NCED gene, designated FvNCED3(KY008746), was cloned from velvet ash(Fraxinus velutina Torr.) with a RACE method. The full length c DNA of FvNCED3 encodes a 573-amino acid polypeptide.Sequencing analysis showed that the FvNCED3 protein was highly homologous to other NCED proteins. The expression patterns of FvNCED3 in different ash organs were analyzed by real-time PCR which revealed that FvNCED3 expression levels were highest in leaves and lowest in roots. The gene expression patterns of FvNCED3 under abiotic stress indicated that its expression increased under drought, salt and ABA stress and decreased due to high and low temperatures. There were no obvious changes under ultraviolet light. The 1094-bp upstream sequence 5' flank regulation region of the FvNCED3 gene was also cloned from ash using the Genome Walking method. To assess the activity of the FvNCED3 promoter, a p FvNCED3 p::GUS plant expression vector was constructed for tobacco transformation. GUS expression of the FvNCED3 GUS enzyme activity was detected in almost all transgenic tobacco tissues, especially in the young leaves,stigma, anther, ovule and ovary. After treating the transgenic tobacco with NaCl and placing it under drought stress, GUS staining of tobacco leaves increased compared with that under normal growth conditions. This result indicates that gene expression driven by the FvNCED3 promoter can be induced by salt and drought stress. | Tian Li Jingkuan Sun Chuanrong Li Zhaohua Lu Jiangbao Xia | 2019 | Journal of Forestry Research2019，30，2： | 6 |
| 4 | Analysis and Optimisation of Halomonas Growth Factors Based on PCA and RSM显示文摘The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium(HDMP1) was isolated and identified. Growth factors were analysed and optimised through the single-factor experiments, the factor analysis(FA), the principal component analysis(PCA), and the response surface methodology(RSM). Results indicated that HDMP1 was identified as genus Halomonas. In the single-factor experiments, the range of suitable growth conditions for HDMP1 covered: a salt concentration of 2%-4%, a medium pH value of approximately 9, an inoculum concentration of 1.0%, a substrate concentration of 1.0%-1.4%, and a rotation rate of 140 r/min. The evaluation by FA and PCA indicated that three significant growth factors were the salt concentration, the pH value, and the rotation rate. A maximum biomass of HDMP1 was obtained under the conditions covering a salt concentration of 3.5%, a medium pH of 8, and a rotation rate of 151 r/min by optimization. | Shi Ke Huang Guofu Xu Huachun Xue Jianliang Sun Jingkuan Xiao Xinfeng Li Lin | 2019 | China Petroleum Processing & Petrochemical Technology2019，21，4： | 1 |
| 5 | The Amygdala Responds Rapidly to Flashes Linked to Direct Retinal Innervation:A Flash-evoked Potential Study Across Cortical and Subcortical Visual Pathways显示文摘Rapid detection and response to visual threats are critical for survival in animals.The amygdala(AMY)is hypothesized to be involved in this process,but how it interacts with the visual system to do this remains unclear.By recording flash-evoked potentials simultaneously from the superior colliculus(SC),lateral posterior nucleus of the thalamus,AMY,lateral geniculate nucleus(LGN)and visual cortex,which belong to the cortical and subcortical pathways for visual fear processing,we investigated the temporal relationship between these regions in visual processing in rats.A quick flash-evoked potential(FEP)component was identified in the AMY.This emerged as early as in the LGN and was approximately 25 ms prior to the earliest component recorded in the SC,which was assumed to be an important area in visual fear.This quick P1 component in the AMY was not affected by restraint stress or corticosterone injection,but was diminished by RU38486,a glucocorticoid receptor blocker.By injecting a monosynaptic retrograde AAV tracer into the AMY,we found that it received a direct projection from the retina.These results confirm the existence of a direct connection from the retina to the AMY,that the latency in the AMY to flashes is equivalent to that in the sensory thalamus,and that the response is modulated by glucocorticoids. | Yanmei Chen Yiling Ni Jianhong Zhou Hua Zhou Qian Zhong Xinyue Li Jichuan Zhang Yuanye Ma Jingkuan Wei | 2021 | Neuroscience Bulletin2021，37，8： | 1 |
| 6 | Nematode faunal response to long-term application of nitrogen fertilizer and organic manure in Northeast China显示文摘 | Wenju Liang Yilai Lou Qi Li Shuang Zhong Xiaoke Zhang Jingkuan Wang | 2008 | Soil Biology and Biochemistry2008，，5： | 1 |
| 7 | Microbial residues as the nexus transforming inorganic carbon to organic carbon in coastal saline soils显示文摘Soil inorganic carbon(SIC),including mainly carbonate,is a key component of terrestrial soil C pool.Autotrophic microorganisms can assimilate carbonate as the main or unique C source,how microorganisms convert SIC to soil organic carbon(SOC)remains unclear.A systematic field survey(n=94)was performed to evaluate the shift in soil C components(i.e.,SIC,SOC,and microbial residues)along a natural salinity gradient(ranging from 0.5‰to 19‰),and further to explore how microbial necromass as an indicator converting SIC into SOC in the Yellow River delta.We observed that SIC levels linearly decreased with increasing salinity,ranging from~12 g kg^(-1)(salinity<6‰)to~10 g kg^(-1)(salinity>6‰).Additionally,the concentrations of SOC and microbial residues exponentially decreased from salinity<6‰ to salinity>6‰,with the decline of 39%and 70%,respectively.Microbial residues and SOC was tightly related to the variations in SIC.The structural equation model showed the causality on explanation of SOC variations with SIC through microbial residues,which can contribute 89% of the variance in SOC storage combined with SIC.Taken together,these two statistical analyses can support that microbial residues can serve as an indicator of SIC transition to SOC.This study highlights the regulation of microbial residues in SIC cycling,enhancing the role of SIC playing in C biogeochemical cycles and enriching organic C reservoirs in coastal saline soils. | Pengshuai Shao Tian Li Kaikai Dong Hongjun Yang Jingkuan Sun | 2022 | Soil Ecology Letters2022，4，4： | 1 |
| 8 | Polylactide/ Graphite Nanosheets/MWCNTs Nanocomposites with Enhanced Mechanical, Thermal and Electrical Proper- ties 显示文摘 | Jingkuan Duan Shuangxi Shao Ya Li etal | 2012 | Iran Polym J2012，21，： | 1 |
| 9 | Influence of Modified Medical Stone on Behavior of Sulfur during Pyrolysis of Longma Coal显示文摘The influence of Co Mo P/medical stone and SO_4^(2-)/medical stone on sulfur behavior during the Longma coal pyrolysis was investigated in a fixed bed reactor. Moreover, the kinetics was also studied. It is found that adding SO_4^(2-)/medical stone was favorable to removal of volatile matter, while adding Co Mo P/medical stone could inhibit the emission of volatiles. Moreover, the results also showed that adding Co Mo P/medical stone made the total sulfur retention higher, while adding SO_4^(2-)/medical stone made the total sulfur retention lower. Adding modified medical stone was beneficial to removal of sulfate sulfur and pyritic sulfur, while it was beneficial to retaining organic sulfur in the residue. Furthermore, adding Co Mo P/medical stone and SO_4^(2-)/medical stone all could increase the emission of H_2S when the temperature was higher than 450℃. Judging from the kinetics study, it also can be known that addition of the natural minerals could result in a decrease of the pre-exponential factor and also change the apparent activation energy upon comparing the apparent activation energy and the pre-exponential factor of raw Longma coal at 435—537℃. | Li Jingkuan Zhao Shuguang Wang Baofeng Jin Yan Zhang Jinjun Cheng Fangqin | 2017 | China Petroleum Processing & Petrochemical Technology2017，19，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员