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| 1 | The effect of nanohybrid materials on the pour-point and viscosity depressing of waxy crude oil显示文摘The pipeline transportation of waxy crude oil is a problem both at home and abroad.In this paper,a novel nanohybrid pour-point depressant (PPD) was used to decrease the pour point and viscosity of waxy crude oil.The pour point and apparent viscosity of waxy crude oil was decreased significantly upon addition of the nanohybrid PPD,and the long-term stability of the nanohybrid PPD was superior to that of a conventional ethylene-vinyl acetate copolymer PPD.Polarized optical microscopy and X-ray diffraction were used to study the effect of the nanohybrid PPD on the crystallization of crude oil.Addition of the nanohybrid PPD reduced the amount of wax crystals,prevented their aggregation,and reduced the temperature at which the crude oil started to crystallize.The significant effect of this nanohybrid PPD on the pour point and viscosity depressing of crude oil is of great importance for facilitating the safe,efficient and energy-minimized transportation of waxy crude oil. | WANG Feng ZHANG DongMin DING YanFen ZHANG LiXin YANG MingShu JIANG BaoLiang ZHANG ShiMin AI MuYang LIU GuangWen ZHI ShuJie HUO LianFeng OUYANG Xin LI Li | 2011 | Chinese Science Bulletin2011，56，1： | 9 |
| 2 | Characterization and allergic role of IL-33-induced neutrophil polarization显示文摘Neutrophils are involved in the pathogenesis of allergy.However,the contribution of the different functionally polarized neutrophils in allergy needs to be clarified.We sought to define the characteristics of interleukin(IL)-33-induced neutrophils and the involvement of this subset of polarized neutrophils in allergic pathogenesis.Freshly isolated neutrophils were treated with different cytokines and the cytokine expression levels were detected by realtime PCR.The gene expression profile of IL-33-induced neutrophils was determined by microarray assay.Adoptive transfer assay was used to investigate the function of IL-33-induced neutrophils in an ovalbumin(OVA)-induced allergic asthma model.IL-33-treated neutrophils selectively produced IL-4,IL-5,IL-9 and IL-13(referred as to N(IL-33)cells)and displayed a distinctive gene expression profile in sharp contrast to resting and lipopolysaccharide(LPS)-treated neutrophils.IL-33-induced neutrophils expressed high Levels of IL-1R2 on cell surface,whereas resting and LPS-treated neutrophils did not,indicating IL-1R2 might be used as a biomarker for N(IL-33)cells.Importantly,N(IL-33)neutrophils exist in the lungs of OVA-induced allergic asthma mice.Adoptive transfer of N(IL-33)neutrophils significantly promotes the severity of the lung pathogenesis in this model.IL-33 induces neutrophil polarization through c-Jun N-terminal kinase-and nuclear factor-κB-dependent pathways.A previously unappreciated neutrophil polarization driven by IL-33 with unique cell surface markers and cytokine/chemokineproducing gene profile was defined.The newly identified N(IL-33)subpopulation may have significant contribution to IL-33-related pathogenesis. | Bo Sun Linnan Zhu Yaling Tao Hai-Xi Sun Yang Li Peng Wang Yuzhu Hou Yang Zhao Xiaodong Zhang Lianfeng Zhang Ning Na Yong Zhao | 2018 | Cellular & Molecular Immunology2018，15，8： | 9 |
| 3 | Interferon-inducible cytoplasmic lncLrrc55-AS promotes antiviral innate responses by strengthening IRF3 phosphorylation显示文摘Type I interferon (IFN-I) production is efficiently induced to ensure a potent innate immune response to viral infection. How this response can be enhanced, however, remains to be explored. Here, we identify a new cytoplasmic long non-coding RNA (lncRNA), lncLrrc55-AS, that drives a positive feedback loop to promote interferon regulatory factor 3 (IRF3) signaling and IFN-I production. We show that lncLrrc55-AS is virus-induced in multiple cell types via the IFN-JAK-STAT pathway. LncLrrc55-AS-deficient mice display a weakened antiviral immune response and are more susceptible to viral challenge. Mechanistically, lncLrrc55-AS binds phosphatase methylesterase 1 (PME-1), and promotes the interaction between PME-1 and the phosphatase PP2A, an inhibitor of IRF3 signaling. LncLrrc55-AS supports PME-1-mediated demethylation and inactivation of PP2A, thereby enhancing IRF3 phosphorylation and signaling. Loss of PME-1 phenocopies lncLrrc55-AS deficiency, leading to diminished IRF3 phosphorylation and IFN-I production. We have identified an IFN-induced lncRNA as a positive regulator of IFN-I production, adding mechanistic insight into lncRNA-mediated regulation of signaling in innate immunity and inflammation. | Yumei Zhou Mengxuan Li Yiquan Xue Zhiqing Li Weitao Wen Xingguang Liu Yuanwu Ma Lianfeng Zhang Zhongyang Shen Xuetao Cao | 2019 | Cell Research2019，29，8： | 8 |
| 4 | Os isotope dating and growth hiatuses of Co-rich crust from central Pacific显示文摘Up to now, accurate determination of the growth age and hiatuses of the Co-rich crust is still a difficult work, which constrains the researches on the genesis, growth process, controlling factors, regional tectonics, paleo-oceanographic background, etc. of the Co-rich crust. This paper describes our work in determining the initial growth age of the Co-rich crust to be of the late Cretaceous Campanian Stage (about 75-80 Ma), by selecting the Co-rich crust with clear multi-layer structures in a central Pacific seamount for layer-by-layer sample analysis and using a number of chronological methods, such as Co flux dating, dating by correlation with 187Os/188Os evolution curves of seawater, and stratigraphic divi- sion by calcareous nannofossils. We have also discovered growth hiatuses with different time intervals in the early Paleocene, middle Eocene, late Eocene and early-middle Miocene, respectively. These re- sults have provided an important age background for further researches on the Co-rich crust growth process and the paleo-oceanographic environment evolution thereby revealed in the said region. | LI JiangShan FANG NianQiao QU WenJun DING Xuan GAO LianFeng WU ChangHang ZHANG ZhenGuo | 2008 | Science China Earth Sciences2008，51，10： | 6 |
| 5 | TRDMT1 exhibited protective effects against LPS-induced inflammation in rats through TLR4-NF-κB/MAPK-TNF-αpathway显示文摘Background:Inflammation is a complex physiological and pathological process.Although many types of inflammation are well characterized,their physiological func-tions are largely unknown.tRNA aspartic acid methyltransferase 1(TRDMT1)has been implicated as a stress-related protein,but its intrinsic biological role is unclear.Methods:We constructed a Trdmt1 knockout rat and adopted the LPS-induced sepsis model.Survival curve,histopathological examination,expression of inflammatory fac-tors,and protein level of TLR4 pathway were analyzed.Results:Trdmt1 deletion had no obvious impact on development and growth.Trdmt1 de-letion slightly increased the mortality during aging.Our data showed that Trdmt1 strongly responded in LPS-treated rats,and Trdmt1 knockout rats were vulnerable to LPS treat-ment with declined survival rate.We also observed more aggravated tissue damage and more cumulative functional cell degeneration in LPS-treated knockout rats compared with control rats.Further studies showed upregulated TNF-αlevel in liver,spleen,lung,and serum tissues,which may be explained by enhanced p65 and p38 phosphorylation.Conclusions:Our data demonstrated that Trdmt1 plays a protective role in inflamma-tion by regulating the TLR4-NF-κB/MAPK-TNF-αpathway.This work provides useful information to understand the TRDMT1 function in inflammation. | Zhengguang Li Xiaolong Qi Xu Zhang Lei Yu Lijuan Gao Weining Kong Wei Chen Wei Dong Lijun Luo Dan Lu Lianfeng Zhang Yuanwu Ma | 2022 | Animal Models and Experimental Medicine2022，5，2： | 5 |
| 6 | Degradome sequencing reveals endogenous small RNA targets in rice (Oryza sativa L. ssp. indica)显示文摘MicroRNAs(miRNAs)and small interfering RNAs(siRNAs)regulate gene expression in eukaryotes.Plant miRNAs modulate their targets mainly via messenger RNA(mRNA)cleavage.Small RNA(sRNA)targets have been extensively investigated in Arabidopsis using computational prediction,experimental validation,and degradome sequencing.However,small RNA targets are largely unknown in rice(Oryza sativa).Here,we report global identification of small RNA targets using high throughput degradome sequencing in the rice indica cultivar 93-11(Oryza sativa L.ssp.indica).One hundred and seventy-seven transcripts targeted by a total of 87 unique miRNAs were identified.Of targets for the conserved miRNAs between Arabidopsis and rice,transcription factors comprise around 70%(58 in 82),indicating that these miRNAs act as masters of gene regulatory nodes in rice.In contrast,non-conserved miRNAs targeted diverse genes which provide more complex regulatory networks.In addition,5 AUXIN RESPONSE FACTORs(ARFs)cleaved by the TAS3 derived ta-siRNAs were also detected.A total of 40 sRNA targets were further validated via RNA ligasemediated 5′rapid amplification of cDNA ends(RLM 5′-RACE).Our degradome results present a detailed sRNAtarget interaction atlas,which provides a guide for the study of the roles of sRNAs and their targets in rice. | Ming ZHOU Lianfeng GU Pingchuan LI Xianwei SONG Liya WEI Zhiyu CHEN Xiaofeng CAO | 2010 | Frontiers in Biology2010，5，1： | 3 |
| 7 | SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice显示文摘Background:Cardiovascular diseases(CVDs)and diabetes mellitus(DM)are top two chronic comorbidities that increase the severity and mortality of COVID-19.However,how SARS-CoV-2 alters the progression of chronic diseases remain unclear.Methods:We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in mice.SARS-CoV-2’s impacts on pathogenesis of chronic diseases were studied through histopathological,virologic and molecular biology analysis.Results:Pre-existing CVDs resulted in viral invasion,ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 infection.Viral infection increased fasting blood glucose and reduced insulin response in DM model.Bone mineral density decreased shortly after infection,which associated with impaired PI3K/AKT/mTOR signaling.Conclusion:We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic diseases.Pre-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection,which further aggravated the pre-existing diseases.This work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection. | Yuanwu Ma Dan Lu Linlin Bao Yajin Qu Jiangning Liu Xiaolong Qi Lei Yu Xu Zhang Feifei Qi Qi Lv Yunpeng Liu Xudong Shi Caixian Sun Jing Li Jie Wang Yunlin Han Kai Gao Wei Dong Ning Liu Shan Gao Jing Xue Qiang Wei Sidan Pan Hong Gao Lianfeng Zhang Chuan Qin | 2021 | Animal Models and Experimental Medicine2021，4，1： | 3 |
| 8 | Characterization and biological significance of IL-23-induced neutrophil polarization显示文摘Neutrophils are heterogeneous with distinct subsets,and can switch phenotypes to exert regulatory functions on immunity.We herein demonstrate that IL-23-treated neutrophils selectively produce IL-17A,IL-17F and IL-22,and display a distinct gene expression profile in contrast to resting and lipopolysaccharide-treated neutrophils.IL-17+neutrophils are present in the colons of mice with dextran sulfate sodium-induced colitis.Adoptive transfer of IL-23-treated neutrophils significantly promotes pathogenesis in this model.IL-23 induces neutrophil polarization through STAT3-dependent RORγt and BATF pathways.Thus,IL-23-induced neutrophil polarization expresses a unique cytokine-producing profile,which may contribute to IL-23-mediated inflammatory diseases. | Yang Li Linnan Zhu Zhulang Chu Tao Yang Hai-Xi Sun Fan Yang Wei Wang Yuzhu Hou Peng Wang Qingjie Zhao Yaling Tao Lianfeng Zhang Xiaodong Zhang Yong Zhao | 2018 | Cellular & Molecular Immunology2018，15，5： | 3 |
| 9 | Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats显示文摘Background:The GGGGCC(G4C2)repeat expansion in the human open reading frame 72 on chromosome 9,C9orf72,is the most common cause of amyotrophic lateral sclerosis(ALS).Studies in transgenic mouse models have linked the pathogenic mechanism of G4C2 repeat expansion to RNA foci or the accumulation of unnatural dipeptide repeats in neurons.However,only one of the existing transgenic mouse lines developed typical ALS.Methods:C9orf72 knockin rats were generated by knockin of 80 G4C2 repeats with human flanking fragments within exon1a and exon1b at the rat C9orf72 locus.Protein expression was detected by western blot.Motor coordination and grip force were measured using a Rotarod test and a grip strength test.Neurodegeneration was assessed by Nissl staining with cresyl violet.Results:C9orf72 haploinsufficiency reduced C9orf72 protein expression 40%in the cerebrum,cerebellum and spinal cords from knockin rats(P<.05).The knockin(KI)rats developed motor deficits from 4 months of age.Their falling latencies and grip force were decreased by 67%(P<.01)and 44%(P<.01),respectively,at 12 months of age compared to wild-type(WT)mice.The knockin of the hexanucleotide repeat expansion(HRE)caused a 47%loss of motor neurons in the spinal cord(P<.001)and 25%(5/20)of female KI rats developed hind limb paralysis at 13 to 24 months.Conclusion:Motor defects in KI rats may result from neurotoxicity caused by HRE and the resulting reduction in C9orf72 protein due to haploinsufficiency.These KI rats could be a useful model for investigating the contributions of loss-of-function to neurotoxicity in C9orf72-related ALS. | Wei Dong Li Zhang Caixian Sun Xiang Gao Feifei Guan Jing Li Wei Chen Yuanwu Ma Lianfeng Zhang | 2020 | Animal Models and Experimental Medicine2020，3，3： | 2 |
| 10 | The relationship between the growth process of the ferromanganese crusts in the Pacific seamount and Cenozoic ocean evolvement显示文摘Base on the Os isotope stratigraphy together with the empirical growth rate models using Co concentrations, the growth ages of the ferromanganese crusts MHD79 and MP3D10 distributed in the seamount of Pacific are confirmed. Through the contrast and research on the previous achievements including ODP Leg 144 and the crusts CD29-2, N5E-06 and N1-15 of the seamount of the Central Pacific, the uniform five growth and growth hiatus periods of them are found, and closely related to the Cenozoic ocean evolvement process. In the Paleocene Carbon Isotope Maximum (PCIM), the rise of the global ocean productivity promoted the growth of the seamount crust; the first growth hiatus (I) of the ferromanganese crust finished. In the Paleocene-Eocene Thermal Maximum (PETM), though the vertical exchange of seawater was weakened, the strong terrestrial chemical weathering led to the input of a great amount of the terrigenous nutrients, which made the bioproductivity rise, so there were no crust hiatuses. During 52-50 Ma, the Early Eocene Optimum Climate (EECO), the two poles were warm, the latitudinal temperature gradient was small, the wind-driven sea circulation and upwelling activity were weak, the terrestrial weathering was also weakened, the open ocean bioproductivity decreased, and the ferromanganese crust had growth hiatus again (II). From early Middle Eocene-Late Eocene, Oligocene, it was a long-term gradually cooling process, the strengthening of the sea circulation and upwelling led to a rise of bioproductivity, and increase of the content of the hydrogenous element Fe, Mn and Co and the biogenous element Cu, Zn, so that was the most favorable stage for the growth of ferromanganese crust (growth periods III and IV) in the studied area. The hiatus III corresponded with the Eocene-Oligocene boundary, is inferred to relate with the global climate transformation, celestial body impact event in the Eocene-Oligocene transition. From the early to the middle Miocene, a large-scale growth hiatus (hiatus period IV) of the ferromanganese crust in the studied area is inferred to relate with temporary warm up climate and ephemeral withdrawal of Antarctic bottom water in the early Miocene. After that, the Antarctic ice sheets extended, the bottom water circumfluence strengthened, the ocean fertility increased, and the once interrupted crust continued to grow in the late Miocene (growth period V). | DING Xuan GAO LianFeng FANG NianQiao QU WenJun LIU Jian LI JiangShan | 2009 | Science China Earth Sciences2009，52，8： | 2 |
| 11 | Landscape and Fruit Developmental Regulation of Alternative Splicing in Tomato by Genome-Wide Analysis显示文摘In eukaryotes, alternative splicing(AS) is one of the posttranscriptional regulatory mechanisms that play important roles by generating transcriptome diversity. To obtain a global view of AS and its dynamics during tomato fruit development, we analyzed the AS events using a large amount of transcriptome datasets. Same with other plant species, about half of the expressed multiexonic genes were alternatively spliced in tomato. Besides that, our further analyzation of RNA-seq datasets of ovule and pericarp at early fruit developmental stages reveals that the dynamic alteration of AS events occurred in specific tissues and AS was regulated spatially and temporally during early fruit development in tomato. By investigating the sequence variations at splice sites causing differential AS events between tomato cultivar ‘Moneymaker' and wild species Solanum pimpinellifolium PI365967, we uncover that AS may play the regulatory roles during domestication of tomato. Taken together, our results provided the global AS pattern in tomato and highlighted the importance of AS during tomato fruit development and domestication. | WANG Ketao JIAO Zhicheng XU Meng WANG Yongsheng LI Ren CUI Xia GU Lianfeng ZHANG Shuaibin | 2016 | Horticultural Plant Journal2016，2，6： | 2 |
| 12 | Effect of Different Cultivation Practices on Soil Physical and Chemical Properties for Greenhouse Vegetables under Long-Term Continuous Cropping显示文摘Five kinds of greenhouse vegetables( eggplant,loofah,tomato,cucumber and pepper) were selected in summer uprooting stage from greenhouse in Shouguang area,Shandong Province. Total nitrogen,ammonium nitrogen,nitrate nitrogen,available phosphorus,available potassium,organic matter content and p H,EC value of three soil layers were measured,respectively. The results showed that the total nitrogen,ammonium nitrogen,nitrate nitrogen,available phosphorus and available potassium in the soil were mainly accumulated in the upper soil,which made the soil acidification trend,because different farmers adopted different cultivation practices for different greenhouse vegetables in Shouguang region,but there was no significant effect on soil EC value. The input of ammonium nitrogen and nitrate nitrogen and other related fertilizers to greenhouse cucumber was higher than that of other greenhouse vegetables,but the amount of available potassium fertilizer and organic matter input to greenhouse tomato under different cultivation practices was lower than other greenhouse vegetables. In Shouguang area,the input of organic matter into greenhouse vegetables should be increased by increasing the proportion of manure input to increase the content of soil organic carbon,so as to achieve the balance of carbon and nitrogen ratio,and to provide a scientific basis for the establishment of an evaluation system for the environmental benefits brought about by chemical fertilizer reduction in greenhouse vegetables in Shouguang area. | Lijuan GAO Guoyuan ZOU Lianfeng DU Shunjiang LI Jing LIU Hongkai DUAN | 2018 | Asian Agricultural Research2018，10，9： | 2 |
| 13 | Research on degradation of penicillins in milk by β-lactamase using ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry显示文摘 | Lixin Li Chunhai Guo Lianfeng Ai Caiyun Dou Guizhen Wang Hanwen Sun | 2014 | Journal of Dairy Science2014，，： | 1 |
| 14 | Determina- tion method of optimal number of clusters for clus- tered wireless sensor networks显示文摘 | Li Wenfeng Martins P Shen Lianfeng | 2012 | Wireless Com- munications and Mobile Computing2012，12，： | 1 |
| 15 | Full-length sequence analysis of hepatitis E virus isolates: showing potential determinants of virus genotype and identity显示文摘 | Dong Yang Mei Jiang Min Jin Zhigang Qiu Weihong Cui Zhiqiang Shen Bo Li Lianfeng Gong Zhaoli Chen Xinwei Wang Jun-Wen Li | 2013 | Virus Genes2013，，3： | 1 |
| 16 | Network design consideration for distributed control systems显示文摘 | LI Lianfeng MOYNE J R | 2002 | IEEE Transactions on Control Systems Technology2002，10，2： | 1 |
| 17 | Performance evaluation of control networks: Ethernet, ControlNet, and DeviceNet显示文摘 | Li Lianfeng Moyne J R Tilbury D M | 2001 | IEEE Control Systems Magazine2001，21，1： | 1 |
| 18 | Performance Evaluation of Control Networks Ethernet, Controlnet and Devicenet显示文摘 | Li Lianfeng Moyne J R Tilburg D M | 2001 | IEEE Control Systemd Magazine2001，2，1： | 1 |
| 19 | Preparation and characterization of 2,3-dialdehyde bacterial cellulose for potential biodegradable tissue engineering scaffolds显示文摘 | Li Jian Wan Yizao Li Lianfeng | 2009 | Materials Science and Engineering2009，29，： | 1 |
| 20 | Preparation and characterization of 2,3-dialdehyde bacterial cellulose for po tential biodegradable tissue engineering scaffolds 显示文摘 | Li Jian Wan Yizao Li Lianfeng | 2014 | Mater Sci Eng C2014，29，5： | 1 |
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