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| 1 | Establishment of an optimized CTC detection model consisting of EpCAM,MUC1 and WT1 in epithelial ovarian cancer and its correlation with clinical characteristics显示文摘Objective:Emerging studies have demonstrated the promising clinical value of circulating tumor cells(CTCs)for diagnosis,disease assessment,treatment monitoring and prognosis in epithelial ovarian cancer.However,the clinical application of CTC remains restricted due to diverse detection techniques with variable sensitivity and specificity and a lack of common standards.Methods:We enrolled 160 patients with epithelial ovarian cancer as the experimental group,and 90 patients including 50 patients with benign ovarian tumor and 40 healthy females as the control group.We enriched CTCs with immunomagnetic beads targeting two epithelial cell surface antigens(EpCAM and MUC1),and used multiple reverse transcription-polymerase chain reaction(RT-PCR)detecting three markers(EpCAM,MUC1 and WT1)for quantification.And then we used a binary logistic regression analysis and focused on EpCAM,MUC1 and WT1 to establish an optimized CTC detection model.Results:The sensitivity and specificity of the optimized model is 79.4%and 92.2%,respectively.The specificity of the CTC detection model is significantly higher than CA125(92.2%vs.82.2%,P=0.044),and the detection rate of CTCs was higher than the positive rate of CA125(74.5%vs.58.2%,P=0.069)in early-stage patients(stage I and II).The detection rate of CTCs was significantly higher in patients with ascitic volume≥500 mL,suboptimal cytoreductive surgery and elevated serum CA125 level after 2 courses of chemotherapy(P<0.05).The detection rate of CTC;and CTC;was significantly higher in chemo-resistant patients(26.3%vs.11.9%;26.4%vs.13.4%,P<0.05).The median progression-free survival time for CTC;patients trended to be longer than CTC;patients,and overall survival was shorter in CTC;patients(P=0.043).Conclusions:Our study presents an optimized detection model for CTCs,which consists of the expression levels of three markers(EpCAM,MUC1 and WT1).In comparison with CA125,our model has high specificity and demonstrates better diagnostic values,especially for early-stage ovarian cancer.Detection of CTC;and CTC;had predictive value for chemotherapy resistance,and the detection of CTC;suggested poor prognosis. | Tongxia Wang Yan Gao Xi Wang Junrui Tian Yuan Li Bo Yu Cuiyu Huang Hui Li Huamao Liang David M.Irwin Huanran Tan Hongyan Guo | 2022 | Chinese Journal of Cancer Research2022，34，2： | 2 |
| 2 | Dose-dependent effects of procyanidin on nerve growth factor expression following cerebral ischemia/reperfusion injury in rats显示文摘BACKGROUND: Recently, grape seed procyanidin (GSP) has been shown to be exhibit antioxidant effects, effectively reducing ischemia/reperfusion injury and inhibiting brain cell apoptosis. OBJECTIVE: To study the effects of GSP on nerve growth factor (NGF) expression and neurological function following cerebral ischemia/reperfusion injury in rats. DESIGN: Randomized controlled study based on SD rats. SETTING: Weifang Municipal People's Hospital. MATERIALS: Forty-eight healthy adult SD rats weighing 280-330 g and irrespective of gender were provided by the Experimental Animal Center of Shandong University. GSP derived from grape seed was a new high-effective antioxidant provided by Tianjin Jianfeng Natural Product Researching Company (batch number: 20060107). Rabbit-anti-rat NGF monoclonal antibody was provided by Beijing Zhongshan Biotechnology Co., Ltd., and SABC immunohistochemical staining kit by Wuhan Boster Bioengineering Co., Ltd. METHODS: The present study was performed in the Functional Laboratory of Weifang Medical College from April 2006 to January 2007. Forty-eight SD rats were randomly divided into the sham operation group, ischemia/reperfusion group, high-dose GSP (40 mg/kg) group, or low-dose GSP (10 mg/kg) group (n = 12 per group). Ischemia/reperfusion injury was established using the threading embolism method of the middle cerebral artery. Rats in the ischemia/reperfusion model group were given saline injection (2 mL/kg i.p.) once daily for seven days pre-ischemia/reperfusion, and once more at 15 minutes before reperfusion. Rats in the high-dose and low-dose GSP groups were injected with GSP (20 or 5 mg/mL i.p., respectively, 2 mL/kg) with the same regime as the ischemia/reperfusion model group. The surgical procedures in the sham operation group were as the same as those in the ischemia/reperfusion model group, but the thread was approximately 10 mm long, thus, the middle cerebral artery was not blocked. MAIN OUTCOME MEASURES: NGF expression in the ischemic penumbra of the temporal cortex was detected by immunohistochemistry, and positive cells counted by light microscopy (×400). The positive cell rate was calculated by [(positive cells/total cells) × 100%]. Neurological function was scored after 2-hour ischemia/48-hour reperfusion. Higher scores reflected more severe neurofunctional defect. RESULTS: The positive rate of NGF expression in all groups receiving ischemia/reperfusion was significantly higher than that in the sham operation group (q=3.87, P < 0.05). The positive rate of NGF expression in the high-dose and low-dose GSP groups were significantly higher than that in the model group (q=4.12, P < 0.05), and were greater in the high-dose compared to low-dose GSP groups (q=4.22, P < 0.05). Neurological function scores in the high-dose and low-dose GSP groups were significantly lower than that in the ischemia/reperfusion model group (q=3.92, P < 0.05). Neurological function score in the high-dose GSP group was significantly less than that in the low-dose GSP group (q=4.02, P < 0.05). CONCLUSION: GSP may up-regulate brain-derived NGF expression in a dose-dependent manner following cerebral ischemia/reperfusion injury in order to improve neurological function and protect the brain. | Feng Li Hai Xie Ying Gao Tongxia Zhan | 2008 | Neural Regeneration Research2008，3，4： | 1 |
| 3 | The Precursory Significance of Cumulative Slip of Repeating Earthquake Sequences Prior to Moderately Strong Earthquakes A Case Study of Four Remarkable Earthquake Sequences of Haicheng- Xiuyan显示文摘The Haicheng-Xiuyan region is an earthquake-prone area in Liaoning Province where earthquake sequences frequently occur and is regarded as the regional seismic window. In this area we found many earthquake events with the highest waveform similarity in the records of the same station from some remarkable seismic sequences,namely repeating earthquake sequences. In principle,rupture areas of the repeating events overlap with each other and are most closely located. Therefore these events may reflect the seismic process near the earthquake fault. In this paper, we identified four remarkable earthquake sequences of Haicheng-Xiuyan by waveform cross-correlation. The result shows that the cumulative slip of repeating earthquakes is related to moderately strong earthquakes,among which the Xiuyan M_S5. 4 foreshock sequence has the strongest and most apparent pre-shock accelerating-like slip behavior. | Li Yutong Zhang Bo Wang Liang Li Tongxia | 2016 | Earthquake Research in China2016，30，1： | 0 |
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