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| 1 | Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand显示文摘It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT)mice and transgenic (TG) mice were treated with acute ethanol (20% v/v) by introperitoneal injection for five times.After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITCconjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was normal in wild-type mice after acute ethanol exposure, suggesting FasL expression determines the sensitivity of testes to ethanol in mice. In summary, we provide the direct evidences that Fas ligand mediates the apoptosis of testicular germ cells induced by acute ethanol using FasL transgenic mice. | JIAHUAHU JIEJIANG YINGHUAMA NAYANG MAOHUZHANG MINWU JIANFEI LIHEGUO | 2003 | Cell Research2003，13，5： | 16 |
| 2 | Cloning and sequencing of mouse GABA transportercomplementary DNA显示文摘A cDNA encoding the mouse GABA transporter has been isolated and sequenced. The results show that the mouse GABA transporter cDNA differs from that of the rat by 60 base pairs at the open reading frame region but the deduced amino acid sequences of the two cDNAs are identical and both composed of 599 amino acids. However,the amino acid sequence is different from the sequence deduced from a recently published mouse GABA transporter cDNA. | TAMANTHONYC.W. LIHEGUO 等 | 1994 | Cell Research1994，4，1： | 1 |
| 3 | Impaired reproduction in transgenic mice overexpressing γ-aminobutyric acid transporter I (GAT1)显示文摘It is well documented that γ-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABAA and GABAB receptors were present in testis and sperm,and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter I (GAT1) also existed in testis and sperm,but its physiological function was unknown. In the present study,we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development,which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover,transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition,testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis,and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment. | JiaHuaHU JinFuZHANG YingHuaMA JieJIANG NaYANG XinBoLI ZhiGuangYUCHI JianFEI LiHeGUO | 2004 | Cell Research2004，14，1： | 0 |
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