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| 1 | Photoperiodism dynamics during the domestication and improvement of soybean显示文摘Soybean(Glycine max) is a facultative short-day plant with a sensitive photoperiod perception and reaction system, which allows it to adjust its physiological state and gene regulatory networks to seasonal and diurnal changes in environmental conditions. In the past few decades, soybean cultivation has spread from East Asia to areas throughout the world. Biologists and breeders must now confront the challenge of understanding the molecular mechanism of soybean photoperiodism and improving agronomic traits to enable this important crop to adapt to geographical and environmental changes. In this review, we summarize the genetic regulatory network underlying photoperiodic responses in soybean. Genomic and genetic studies have revealed that the circadian clock, in conjunction with the light perception pathways, regulates photoperiodic flowering. Here, we provide an annotated list of 844 candidate flowering genes in soybean, with their putative biological functions. Many photoperiod-related genes have been intensively selected during domestication and crop improvement. Finally, we describe recent progress in engineering photoperiod-responsive genes for improving agronomic traits to enhance geographic adaptation in soybean, as well as future prospects for research on soybean photoperiodic responses. | Sheng-Rui Zhang Huan Wang Zhongyu Wang Yao Ren Lifang Niu Jun Liu Bin Liu | 2017 | Science China(Life Sciences)2017，60，12： | 13 |
| 2 | Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma显示文摘Background:Resistance to ferroptosis,a regulated cell death caused by irondependent excessive accumulation of lipid peroxides,has recently been linked to lung adenocarcinoma(LUAD).Intracellular antioxidant systems are required for protection against ferroptosis.The purpose of the present studywas to investigate whether and how extracellular system desensitizes LUAD cells to ferroptosis.Methods:Established human lung fibroblasts MRC-5,WI38,and human LUAD H1650,PC9,H1975,H358,A549,and H1299 cell lines,tumor and matched normal adjacent tissues of LUAD,and plasma from healthy individuals and LUAD patients were used in this study.Immunohistochemistry and immunoblotting were used to analyze protein expression,and quantitative reverse transcription-PCR was used to analyze mRNA expression.Cell viability,cell death,and the lipid reactive oxygen species generationwere measured to evaluate the responses to ferroptosis.Exosomes were observed using transmission electron microscope.The localization of arachidonic acid(AA)was detected using click chemistry labeling followed by confocal microscopy.Interactions between RNAs and proteins were detected using RNA pull-down,RNA immunoprecipitation and photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation methods.Proteomic analysis was used to investigate RNA-regulated proteins,and metabolomic analysis was performed to analyze metabolites.Cellderived xenograft,patient-derived xenograft,cell-implanted intrapulmonary LUAD mouse models and plasma/tissue specimens from LUAD patients were used to validate the molecular mechanism.Results:Plasma exosome from LUAD patients specifically reduced lipid peroxidation and desensitized LUAD cells to ferroptosis.A potential explanation is that exosomal circRNA_101093(cir93)maintained an elevation in intracellular cir93 in LUAD to modulate AA,a poly-unsaturated fatty acid critical for ferroptosisassociated increased peroxidation in the plasma membrane.Mechanistically,cir93 interacted with and increased fatty acid-binding protein 3(FABP3),which transported AA and facilitated its reaction with taurine.Thus,global AA was reduced,whereas N-arachidonoyl taurine(NAT,the product of AA and taurine)was induced.Notably,the role of NAT in suppressing AA incorporation into the plasma membrane was also revealed.In pre-clinical in vivo models,reducing exosome improved ferroptosis-based treatment.Conclusion:Exosome and cir93 are essential for desensitizing LUAD cells to ferroptosis,and blocking exosome may be helpful for future LUAD treatment. | Xiao Zhang Yunhua Xu Lifang Ma Keke Yu Yongjie Niu Xin Xu Yi Shi Susu Guo Xiangfei Xue Yikun Wang Shiyu Qiu Jiangtao Cui Hong Wang Xiaoting Tian Yayou Miao Fanyu Meng Yongxia Qiao Yongchun Yu Jiayi Wang | 2022 | Cancer Communications2022，42，4： | 10 |
| 3 | AGAMOUS AND TERMINAL FLOWER controls floral organ identity and inflorescence development in Medicago truncatula显示文摘Angiosperms integrate a multitude of endogenous and environmental signals to control floral development,thereby ensuring reproductive success.Here,we report the identification of AGAMOUS AND TERMINAL FLOWER(AGTFL),a novel regulator of floral development in Medicago truncatula.Mutation of AGTFL led to the transformation of carpels and stamens into numerous sepals and petals and altered primary inflorescence identity.AGTFL encodes a nucleus-localized proteincontaining a putative Myb/SANT-like DNA-binding domain and a PKc kinase domain.Molecular and genetic analyses revealed that AGTFL regulates the transcription of MtAGs and MtTFL1 to control floral organ identity and inflorescence development. | Butuo Zhu Hui Li Yifeng Hou Pengcheng Zhang Xiuzhi Xia Na Wang Hui Wang Kirankumar S.Mysore Jiangqi Wen Yanxi Pei Lifang Niu Hao Lin | 2019 | Journal of Integrative Plant Biology2019，61，8： | 2 |
| 4 | The enzymatic activity of Arabidopsis protein arginine methyltransferase 10 is essential for flowering time regulation显示文摘Arabidopsis AtPRMT10 is a plant-specific type I protein arginine methyltransferase that can asymmetrically dimethylate arginine 3 of histone H4 with auto-methylation activity.Mutations of AtPRMT10 derepress FLOWERING LOCUS C(FLC)expression resulting in a late-flowering phenotype.Here,to further investigate the biochemical characteristics of AtPRMT10,we analyzed a series of mutated forms of the AtPRMT10 protein.We demon-strate that the conserved“VLD”residues and“double-E loop”are essential for enzymatic activity of AtPRMT10.In addition,we show that Arg54 and Cys259 of AtPRMT10,two residues unreported in animals,are also important for its enzymatic activity.We find that Arg13 of AtPRMT10 is the auto-methylation site.However,substitution of Arg13 to Lys13 does not affect its enzymatic activity.In vivo complementation assays reveal that plants expressing AtPRMT10 with VLD-AAA,E143Q or E152Q mutations retain high levels of FLC expression and fail to rescue the late-flowering phenotype of atprmt10 plants.Taken together,we conclude that the methyltransferase activity of AtPRMT10 is essential for repressing FLC expression and promoting flowering in Arabidopsis. | Lifang Niu Falong Lu Taolan Zhao Chunyan Liu Xiaofeng Cao | 2012 | Protein & Cell2012，3，6： | 1 |
| 5 | Aggregation-induced reversible thermochromism of novel azo chromophore-func- tionalized polydiacetylene cylindrical micelles 显示文摘 | Wu Si Niu Lifang Shen Jing | 2009 | Macro- molecules2009，42，1： | 1 |
| 6 | DPB3 and DPB4 proteins regulate Medicago flowering and leaf anthocyanin biosynthesis显示文摘As important subunits of the leading-strand DNA polymerase epsilon,chromatin remodeling,and histone acetylation complexes,the H2A/H2B-like histone-fold domain-containing proteins DNA PO-LYMERASE II SUBUNIT B3(DPB3)and DPB4 play key roles in nucleosome assembly and heterochromatin maintenance during DNA replication in yeast,Drosophila,and mammals(He et al.,2017;Bellelli et al.,2018;Yu et al.,2018;Casari et al.,2021). | Yingying Meng Chongnan Wang Qiqi Li Wenkai Ji Jiangqi Wen Kirankumar S.Mysore Yanxi Pei Lifang Niu Hao Lin | 2023 | Journal of Genetics and Genomics2023，50，6： | 0 |
| 7 | Co-expression of GR79 EPSPS and GAT generates highglyphosate-resistant alfalfa with low glyphosate residues显示文摘Weed competition seriously threatens the yield of alfalfa, the most important forage legume worldwide,thus generating herbicide-resistant alfalfa varieties is becoming a necessary cost-effective strategy toassist farmers for weed control. Here, we report the co-expression of plant codon-optimized forms ofGR79 EPSPS (pGR79 EPSPS) and N-acetyltransferase (pGAT) genes, in alfalfa, via Agrobacterium-mediated transformation. We established that the pGR79 EPSPS-pGAT co-expression alfalfa lines were able totolerate up to tenfold higher commercial usage of glyphosate and produced approximately ten timeslower glyphosate residues than the conventional cultivar. Our findings generate an elite herbicideresistant germplasm for alfalfa breeding and provide a promising strategy for developing high-glyphosate-resistant and low-glyphosate-residue forages. | Yingying Meng Wenwen Zhang Zhaoming Wang Feng Yuan Sandui Guo Hao Lin Lifang Niu | 2023 | aBIOTECH2023，4，4： | 0 |
| 8 | Clinical phenotype features and genetic etiologies of 38 children with progressive myoclonic epilepsy显示文摘Background:Progressive myoclonic epilepsy(PME)is a group of neurodegenerative diseases with genetic heterogeneity and phenotypic similarities,and many cases remain unknown of the genetic causes.This study is aim to summarize the clinical features and study the genetic causes of PME patients.Methods:Sanger sequencing of the target gene,Next Generation Sequencing(NGS)panels of epilepsy,trio-based Whole Exome Sequencing(WES)and detection of cytosine-adenine-guanine(CAG)repeat number were used to investigate the genetic causes of PME patients.Results:Thirty-eight children with PME whose seizure onset age ranged from 3 months to 12 years were collected from February 2012 to November 2019 in three hospitals in Beijing,China.The seizure types included myoclonic seizures(n=38),focal seizures(n=19),generalized tonic-clonie seizure(GTCS)(n=13),absence seizures(n=4),atonic seizures(n=3),epileptic spasms(n=2)and tonic seizures(n=1).Twenty-seven cases were sporadic and 11 had family members affected.Established PME-related genes were identified in 30 out of 38(78.9%)patients who had either recessively inherited or de novo heterozygous mutations.Among these 30 cases,there were 12 cases(31.6%)of neuronal ceroid lipofuscinoses(the causing gene contains TPP1,PPT1,CLN5,CLN6 and MFSD8),two cases of sialidosis(the causing gene is NEU1),two cases of neuronopathic Gaucher disease(the causing gene is GBA),one case of spinal muscular atrophy-progressive myoclonic epilepsy(the causing gene is ASAH1),four cases of KCNC1 mutation-related PME,four cases of KCTD7 mutation-related PME,two cases of TBC1D24 mutation-related PME,one case of GOSR2 related PME,and two of dentatorubral-pallidoluysian atrophy(the causing gene is ATN1).In total,13 PME genes were identified in our cohort.The etiology was not clear in eight patients.Conclusion:PME is a group of clinically and genetically heterogeneous diseases.Genetic diagnosis was clear in 78.9%of PME patients.Various of genetic testing methods could increase the rate of genetic diagnosis.Neuronal ceroid lipofuscinoses(NCL)is the most common etiology of PME in children.Nearly one third PME children were diagnosed with NCL.GOSR2 related PME was in our cohort in Asia for the first time. | Jing Zhang Ying Yang Xueyang Niu Jiaoyang Chen Wei Sun Changhong Ding Lifang Dai Liping Zhang Qi Zeng Yi Chen Xiaojuan Tian Xiaoling Yang Taoyun Ji Zhixian Yang Yanling Yang Yuwu Jiang Yuehua Zhang | 2020 | Acta Epileptologica2020，2，1： | 0 |
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