2篇
您的检索式：作者名="Linglong CHENG"
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| 1 | Exploring the heavy air pollution in Beijing in the fourth quarter of 2015: assessment of environmental benefits for red alerts显示文摘 | Teng NIE Lei NIE Zhen ZHOU Zhanshan WANG Yifeng XUE Jiajia GAO Xiaoqing WU Shoubin FAN Linglong CHENG | 2018 | Frontiers of Earth Science2018，12，2： | 2 |
| 2 | Functional characterization of CYP81C16 involved in the tanshinone biosynthetic pathway in Salvia miltiorrhiza显示文摘Danshen,the dried roots and rhizomes of Salvia miltiorrhiza Bunge(S.miltiorrhiza),is widely used in the treatment of cardiovascular and cerebrovascular diseases.Tanshinones,the bioactive compounds from Danshen,exhibit a wide spectrum of pharmacological properties,suggesting their potential for future therapeutic applications.Tanshinone biosynthesis is a complex process involving at least six P450 enzymes that have been identified and characterized,most of which belong to the CYP76 and CYP71 families.In this study,CYP81C16,a member of the CYP71 clan,was identified in S.miltiorrhiza.An in vitro assay revealed that it could catalyze the hydroxylation of four para-quinone-type tanshinones,namely neocryptotanshinone,deoxyneocryptotanshinone,and danshenxinkuns A and B.SmCYP81C16 emerged as a potential broad-spectrum oxidase targeting the C-18 position of para-quinone-type tanshinones with an impressive relative conversion rate exceeding 90%.Kinetic evaluations and in vivo assays underscored its highest affinity towards neocryptotanshinone among the tested substrates.The overexpression of SmCYP81C16 promoted the accumulation of(iso)tanshinone in hairy root lines.The characterization of SmCYP81C16 in this study accentuates its potential as a pivotal tool in the biotechnological production of tanshinones,either through microbial or plant metabolic engineering. | REN Li LUO Linglong HU Zhimin MA Ying WANG Jian CHENG Yatian JIN Baolong CHEN Tong TANG Jinfu CUI Guanghong GUO Juan HUANG Luqi | 2023 | Chinese Journal of Natural Medicines2023，21，12： | 0 |
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