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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Epidemiologic characteristics of Helicobacter pylori infection in southeast Hungary显示文摘BACKGROUND Epidemiologic studies have revealed a decrease in the prevalence of Helicobacter pylori(H.pylori)infection in Western Europe.AIM To obtain data regarding the prevalence of H.pylori in Csongrád and Békés Counties in Hungary,evaluate the differences in its prevalence between urban and rural areas,and establish factors associated with positive seroprevalence.METHODS One-thousand and one healthy blood donors[male/female:501/500,mean age:40(19–65)years]were enrolled in this study.Subjects were tested for H.pylori IgG antibody positivity via enzyme-linked immunosorbent assay.Subgroup analysis by age,gender,smoking habits,alcohol consumption,and urban vs nonurban residence was also performed.RESULTS The overall seropositivity of H.pylori was 32%.It was higher in males(34.93%vs 29.2%,P=0.0521)and in rural areas(36.2%vs 27.94%,P=0.0051).Agricultural/industrial workers were more likely to be positive for infection than office workers(38.35%vs 30.11%,P=0.0095)and rural subjects in Békés County than those in Csongrád County(43.36%vs 33.33%,P=0.0015).CONCLUSION Although the prevalence of H.pylori infection decreased in recent decades in Southeast Hungary,it remains high in middle-aged rural populations.Generally accepted risk factors for H.pylori positivity appeared to be valid for the studied population. | Lenke Bálint Andrea Tiszai Gábor Kozák Ilona Dóczi Veronika Szekeres Orsolya Inczefi Georgina Ollé Krisztina Helle Richárd Róka András Rosztóczy | 2019 | World Journal of Gastroenterology2019，25，42： | 5 |
| 3 | Biomarker evidence for widespread anaerobic methane oxidation in Mediterranean sediments by a consortium of methanogenic archaea and bacteria显示文摘 | Pancost R D Sinninghe Damste J S De Lint S | 2000 | Appl Environ Microbiol2000，66，： | 1 |
| 4 | HIV persistence and the prospect of long-term drug-free remissions for HIV-infected individuals显示文摘 | Trono D Van Lint C Rouzioux C Verdin E Barre-Sinoussi F Chun TW Chomont N | 2010 | Science2010，329，5988： | 1 |
| 5 | Application of the charge regulation model to transport of ions through hydrophilic membranes: one-dimensional transport model for narrow pores (nanofiltration) 显示文摘 | Lint D Biesheuvel W B Verweij P M | 2002 | Joumal of Colloid and Interface Science2002，251，1： | 1 |
| 6 | Xor-based visual cryptography schemes显示文摘 | Tuyls P Hollmann H D Van Lint J H | 2005 | Designs Codes and Cryptography2005，37，1： | 1 |
| 7 | The predictive power of the yield curve: a theoretical assessment显示文摘 | Lint C R Stolin D | 2003 | Journal of Monetary Economics2003，50，3： | 1 |
| 8 | XOR-based visual cryptography schemes显示文摘 | TUYLS P HOLLMANN H D L LINT van J H | 2005 | Designs Codes and Cryptography2005，37，1： | 1 |
| 9 | Biomarker evidence for widespread anaerobic oxidation in Mediterranean sediments by a consortium of methanogenic archaea and bacteria显示文摘 | Pancost R D Sinninghe Damste′ J S de Lint S | 2000 | Applied Environmental Microbiology2000，66，： | 1 |
| 10 | Biomarker evidence for widespread anaerobic methane oxidation in Mediter- ranean sediments by a consortium of methanogenic Archaea and Bacteria显示文摘 | Paneost R D Sinninghe Damste J S de Lint S | 2000 | Applied and Environmental Microbiology2000，66，3： | 1 |
| 11 | On Codes with the Identifiable Parent Property显示文摘 | Hollmann H D L Van Lint J H Linnartz J P | | 0，，： | 1 |
| 12 | HIV persistence and the prospect of long-term drug-free remissions for HIV-infected individuals显示文摘 | Trono D Van Lint C Rouzioux C | 2010 | Science2010，329，5988： | 1 |
| 13 | Increased risk of leukemia relapse with high-dose cyelosporine after allogeneic marrow transplantation for acute leukemia:10-year follow-up of a randomized study显示文摘 | Bacigalupo A van Lint MT Occhini D | 2001 | Blood2001，98，： | 1 |
| 14 | Molecular cloning and charac- terization of a novel transglutaminase cDNA from a hu- man prostate cDNA library显示文摘 | Grant F J Taylor D A Sheppard P O Mathewes S L Lint W Vanaja E | 1994 | Biochem Biophys Res Commun1994，203，： | 1 |
| 15 | Variability and transport of suspended sediment, particulate and dissolved organic carbon in the tidal freshwater Hudson River 显示文摘 | Findlay S Pace M L Lints D | 1991 | Biogeochemistry1991，12，： | 1 |
| 16 | Successful transcutaneous electrical nerve stimulation in two women with restless genital syndrome: the role of adelta- and C-nerve fibers 显示文摘 | Waldinger M D de Lint G J Venema P L | 2010 | J Sex Med2010，7，3： | 1 |
| 17 | XOR-based visual cryptography schemes显示文摘 | Tuyls P Hollmann H D L Lint J H V | 2005 | Designs Codes and Cryptography2005，37，1： | 1 |
| 18 | A review of spinning, shear forming and flow forming processes mechanicaland manufacturing en- gineering 显示文摘 | WONG C C LINT D J | 2003 | International Journal of Machine tOOls &Manufacture2003，43，14： | 1 |
| 19 | On codes with the identifiable parent property显示文摘 | Hollmann H D L van Lint J H Linnartz J P | 1998 | Journal of Combinatorial Theory A1998，82，： | 1 |
| 20 | Flexure and Shear Behavior of Concrete Beams During Fires显示文摘 | ELLINGWOOD B LINT D | 1991 | Journal of Structural Engineering1991，117，2： | 1 |
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