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| 1 | Regulatory mechanisms for abnormal expression of the human breast cancer specific gene 1 in breast cancer cells显示文摘Breast cancer-specific gene 1 (BCSG1), also referred as synuclein γ, was originally iso-lated from a human breast cancer cDNA library and the protein is mainly localized to presynaptic terminals in the nervous system. BCSG1 is not expressed in normal or benign breast lesions, but expressed at an extremely high level in the vast majority of the advanced staged breast carcinomas and ovarian carcinomas. Overexpression of BCSG1 in cancer cells led to significant increase in cell proliferation, motility and invasiveness, and metastasis. To elucidate the molecular mechanism and regulation for abnormal transcription of BCSG1, a variety of BCSG1 promoter luciferase reporters were constructed including 3′ end deleted sequences, Sp1 deleted, and activator protein-1 (AP1) domains mutated. Transient transfection assay was used to detect the transcriptional activation of BCSG1 promoters. Results showed that the Sp1 sequence in 5′-flanking region was involved in the basal transcriptional activities of BCSG1 without cell-type specificity. In comparison to pGL3-1249, the reporter activities of pGL3-1553 in BCSG1-negative MCF-7 cells and pGL3-1759 in HepG2 cells were notably decreased. Mutations at AP1 sites in BCSG1 intron 1 significantly reduced the promoter ac-tivity in all cell lines. Transcription factors, c-jun, c-fos and cyclin AMP-responsive element binding (CREB) protein, could markedly enhance the promoter activities. Thus, our results suggest that the abnormal expression of BCSG1 in breast cancer cells is likely regulated by multiple mechanisms. The 5′ flanking region of BCSG1 provides the basal transcriptional activity without cell type specificity. A critical promoter element involved in abnormal expression of BCSG1 presents in the first exon. The cell type specificity of BCSG1 transcription is probably affected through intronic cis-regulatory se-quences. AP1 domains in the first intron play an important role in control of BCSG1 transcription. | LU Aiping1, LI Qing1, LIU Jingwen2 1. Department of Pathology, Peking University School of Oncology, Beijing Cancer Hospital, Beijing 100036, China 2. VA Palo alto Health Care System, Palo Alto, California, CA 94304, USA | 2006 | Science China(Life Sciences)2006，49，4： | 8 |
| 2 | Oncolytic vaccinia virotherapy for endometrial cancer显示文摘 | Liu YP Wang J Avanzato VA | 2014 | Gynecol Oncol2014，132，3： | 1 |
| 3 | N-acetyl-GLP-1:a DPP IV-resistant analogue of glucagon-like peptide-1 (GLP-1) with improved effects on pancreatic beta-cell-associated gene expression显示文摘 | Liu HK Green BD Gault VA | 2004 | Cell Biol Int2004，28，1： | 1 |
| 4 | Engineering protein and cell adhesivity using PEO-terminated triblock polymers显示文摘 | Liu VA Jastromb WE Bhatia SN | 2002 | J Biomed Mater Res2002，60，1： | 1 |
| 5 | Maternal prenatal urinary phthalate metabolite concentrations and child mental,psychomo- tor, and behavioral development at 3 years of age 显示文摘 | Whyatt RM Liu X Rauh VA | 2012 | Environ Health Perspect2012，120，2： | 1 |
| 6 | Down-Regulation of miR-29b is essential for Pathogenesis of uterine Leiomyoma显示文摘 | Qiang W Liu Z Serna VA | 2014 | Endocrinology2014，155，3： | 1 |
| 7 | The telomerase/vault associated protein TEP1 is required for vault RNA stability and its association with the vault particle显示文摘 | Kickhoefer VA Liu Y Kong LB | 2001 | J Cell Biol2001，152，1： | 1 |
| 8 | Maternal prenatal urinary phthalate metabolite concentrations and child mental, psychomotor and behavioral development at age three years显示文摘 | Whyatt RM Liu X Rauh VA | 2011 | Environ Health Perspect2011，120，2： | 1 |
| 9 | Vault poly(ADP-ribose) polymerase is associated with mammalian telomerase and is dispensable for telomerase function and vault structure in vivo显示文摘 | Liu Y Snow BE Kickhoefer VA | 2004 | Mol Cell Biol2004，24，12： | 1 |
| 10 | Caregiver burden and coping patterns of Chinese parents o{ a child with a mental illness显示文摘 | Liu M Lambert CE Lambert VA | 2007 | Int J Ment Health Nurs2007，16，2： | 1 |
| 11 | Caregiver burden and coping patterns of Chinese parents of a child with a mental illness 显示文摘 | Liu M Lambert CE Lambert VA | 2007 | Int J Ment Health Nuts2007，16，2： | 1 |
| 12 | Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots显示文摘 | Liu J Lau SK Varma VA | | 0，，05： | 1 |
| 13 | Multiplexed detection and characterization of rare tumor cells in Hodgkin's lymphomawith multicolor quantum dots显示文摘 | Liu J Lau SK Varma VA | | 0，，： | 1 |
| 14 | Elevated levels of Snitrosoalbumin in preeclampsia plasma显示文摘 | Tyurin VA Liu SX Tyurina YY | 2001 | Circ Res2001，88，： | 1 |
| 15 | Telomere, telomerase and aging显示文摘 | Liu Y Bohr VA Lansdorp P | 2008 | Mech Ageing Dev2008，129，12： | 1 |
| 16 | High-volume impactor for sampling fine and coarse particles显示文摘 | Marple VA Liu BYH Burton RM | 1990 | J Air Waste Manage1990，40，： | 1 |
| 17 | Isolation,kinetic analysis,and structural characterization of an antibody targeting the Bacillus anthracis major spore surface protein BclA显示文摘 | Nuttall SD Wilkins ML Streltsov VA Pontes-Braz L Dolezal O Tran H Liu CQ | | 0，，： | 1 |
| 18 | The Telomerase/vault - associated protein TEP1 is required for vault RNA stability and its association with the vault particle显示文摘 | Kickhoefer VA Liu Y Kong LB | 2001 | J Cell Biol2001，152，1： | 1 |
| 19 | The prognostic role of loss of insulin-like growth factor-binding protein-3 expression in head and neck carcinogenesis显示文摘 | Papadimitrakopoulou VA Brown EN Liu DD | 2006 | Cancer Lett2006，239，1： | 1 |
| 20 | Caregiver burden and copingpatterns of Chinese parents of a child with a mental illness显示文摘 | Liu M Lambert C E Lambert VA | 2007 | IntJ Ment Health Nurs2007，16，2： | 1 |
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