| 4 | Determination of scutellarin in plasma by solid phase extraction—high performance liquid chromatography and its pharmacokinetics显示文摘Breviscapine has extensive effects and its main active component is scutellarin.This study established a solid phase extraction-high performance liquid chromatography (SPE-HPLC) method for determining scutellarin in plasma,and studied its pharmacokinetics after iv breviscapine in rabbits.Methanol-water-phosphoric acid was used as mobile phase,Nucleosil C18 column was welected.Plasma samples were pretreated by solid phase extraction.Fifteen rabbits(3 groups) were given breviscapine by iv at the dose of 10.0,20.0 and 40.0mg·kg^-1,respectively.Scutellarin in plasma was determined.The linearity was obtained over the range of 0.02-10.0mg·L^-1.The limit of detection was 0.02mg·L^-1.After iv breviscapine in rabbits,the concentration-time curves of scutellarin was fitted to three compartment model.The results showed that this assay method was accurate,sensitive and simple.The changes of drug concentration in vivo exhibited linear kinetics over the dosage range of 10.0-20.0mg·kg^-1,but when dosage was 40.0mg·kg^-1,the linear kinetic properties did not exist. | LiuYM LiuAH | 2002 | 中国药理学与毒理学杂志2002，16，6： | 0 |
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