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| 1 | The process and driving forces of rural hollowing in China under rapid urbanization显示文摘农村凹陷是在中国收到了重要注意的最近的地理现象,它正在经历快速的都市化。它导致了农村陆地资源的浪费使用,并且在陆地使用的优化上强加了障碍并且协调了城市农村的开发。农村凹陷有表明的各种各样的形式,它指农村住所的疏忽和空缺,哪个能导致损坏和农村住所的最终的放弃。损坏住所有破坏的不同的度,从忽视,对中等严重。一般来说的农村凹陷的发展过程有五个阶段,即,出现,生长,繁荣,稳定性,和衰落。基于两地区性的经济发展水平和它的地文学的特征的联合,农村凹陷的类型能作为城市的穗,平凡农业区域,多山的农业区域,和 agro 牧剧区域被分类。特别,平凡农业区域是在农村凹陷的最典型的,它显示出与一个分层的空核心和稳固的形状的作为一个水煮的鸡蛋模式的农村凹陷的空间进化。而且,在农村凹陷后面的驱动力作为城市的拉和农村区域的推被识别。特别地,这份报纸识别贡献者到农村凹陷在与快速的 urbanisation 和经济变化的关系包括农村人口减少,打所有权并且打使用政策,和机构的障碍。 | LIU Yansui LIU Yu CHEN Yangfen LONG Hualou | 2010 | Journal of Geographical Sciences2010，20，6： | 100 |
| 2 | Multimodality treatment in hepatocellular carcinoma patients with tumor thrombi in portal vein显示文摘AIM To compare the therapeutic effect andsignificances of multimodality treatment forhepatocellular carcinoma (HCC) with tumorthrombi in portal vein (PVTT).METHODS HCC patients (n = 147) with tumortrombi in the main portal vein or the first branchof portal vein were divided into four groups bythe several therapeutic methods. There wereconservative treatment group in 18 out ofpatients (group A); and hepatic artery ligation(HAL) and/or hepatic artery infusion (HAl)group in 18 patients (group B), in whompostoberative chemoembolization was doneperiodically; group of removal of HCC with PVTTin 79 (group C) and group of transcatheterhepatic arterial chemoembolization (TACE) orHAl and/or portal vein infusion (PVI) afteroperation in 32 (group D).RESULTS The median survival period was 12months in our series and the 1-, 3-, and 5-yearsurvival rates were 44.3%, 24.5% and 15.2%,respectively. The median survival times were 2,5, 12 and 16 months in group A, B, C and D,respectively. The 1-, 3- and 5-year survival rateswere 5.6%, 0% and 0% in group A; 22.2%,5.6% and 0% in group B; 53.9%, 26.9% and16.6% in group C; 79.3%, 38.9% and 26.8% ingroup D, respectively. Significant differenceappeared in the survival rates among the groups(P<0.05).CONCLUSION Hepatic resection with removalof tumor thrombi and HCC should increase thecurative effects and be encouraged for theprolongation of life span and quality of life forHCC patients with PVTT, whereas the besttherapeutic method for HCC with PVTT is withregional hepatic chemotherapy orchemoemblization after hepatic resection withremoval of tumor thrombi. | Jia Fan Zhi Quan Wu Zhao You Tang Jian Zhou Shuang Jian Qiu Zeng Chen Ma Xin Da Zhou Sheng Long Ye Liver Cancer Institute, Zhongshan Hospital, Fudan University Medical Center (Former Shanghai University), 136 Yixueyuan Road, Shanghai 200032, China | 2001 | World Journal of Gastroenterology2001，7，1： | 80 |
| 3 | Surface Rupture and Co-seismic Displacement Produced by the Ms 8.0 Wenchuan Earthquake of May ^(12)th,2008,Sichuan,China:Eastwards Growth of the Qinghai-Tibet Plateau显示文摘女士 8 的地震给 Wenchuan 以县,西方的四川,中国,在 2008 年 5 月 12 日并且导致了长表面破裂(> 300 km ) 。关于表面破裂的第一手的观察在 Yingxiu, Beichuan 和 Qingchuan 的最糟点击的区域由地震生产了,查明地震的原因的结构在 Longmenshan 构造的带的中央差错地区。沿着 Yingxiu-Beichuan 破裂地区活动范围 2.5-4 m 的单个差错的平均 co 地震的垂直排水量和越过中央、正面的 Longmenshan 差错的累积垂直排水量系上带子是大约 56 m。表面破裂力量从 Beichuan 的北方被归结为 Qingchuan 县和表演 23 m 右罢工滑倒部件。Wenchuan 指责戳的地震是在印度、欧亚的大陆的连续集中的行动下面的西藏的高原的东方生长的表明。 | DONG Shuwen ZHANG Yueqiao WU Zhenhan YANG Non MA Yinsheng SHI Wei CHEN Zhengle LONG Changxin AN Meijian | 2008 | Acta Geologica Sinica(English Edition)2008，82，5： | 59 |
| 4 | Jurassic Tectonic Revolution in China and New Interpretation of the “Yanshan Movement”显示文摘摘要:与结构的地质的调查的新数据和高分辨率的同位素的标明日期数据的获得和累积,我们极大地改进了我们从迟了的侏罗记在时期期间发生在东方中国到早白垩纪的构造事件的理解并且可以给性质的新解释,预定并且 Yanshan 运动的 geodynamic 背景。在中间迟了的侏罗记期间(165 挠慬獳漠 ?? | DONG Shuwen ZHANG Yueqiao LONG Changxing YANG Zhenyu JI Qiang WANG Tao HU Jianmin CHEN Xuanhua | 2008 | Acta Geologica Sinica(English Edition)2008，82，2： | 41 |
| 5 | Expression,deleton and mnutation of ρ16 gene in human gastric cancer显示文摘AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P<0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41 ),undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/ 16) (P<0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P<0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis. | Xiu-Sheng He Qi Su Zhu-Chu Chen Xiu-Tao He Zhi-Feng Long Hui Ling Liang-Run Zhang Oncology Institute,Nanhua University,Hengyang 421001,Hunan Province,ChinaOncology Institute,Center South University,Changsha 410078,Hunan Province,China Department of Gastroenterology,First People’s Hospital of Changde City,Changde 415003,Hunan Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 40 |
| 6 | Uterine artery embolization in cesarean scar pregnancy: safe and effective intervention显示文摘 | Cao Shasha Zhu Lihong Jin Long Gao Jian Chen Chen | 2014 | Chinese Medical Journal2014，，12： | 37 |
| 7 | Screening test for anti-Helicobacter pylori activity of traditional Chinese herbal medicines显示文摘AIM:To evaluate the anti-Helicobacter pylori (H.pylori) activity of 50 traditional Chinese herbal medicines in order to provide the primary evidence for their use in clinical practice.METHODS:A susceptibility test of water extract from 50 selected traditional Chinese herbal medicines for in vitro H.pylori Sydney strain 1 was performed with broth dilution method.Anti-H.pylori activity of the selected Chinese herbal medicines was evaluated according to their minimum inhibitory concentration (MIC).RESULTS:The water extract from Rhizoma Coptidis,Radix Scutellariae and Radix isatidis could significantly inhibit the H.pylori activity with their MIC less than 7.8 mg/mL,suggesting that traditional Chinese herbal medicines have anti-inflammatory and antibacterial effects and can thus be used in treatment of H.pylori infection.CONCLUSION:Rhizoma Coptidis,Radix Scutellariae and Radix isatidis are the potential sources for the synthesis of new drugs against H.pylori. | Feng Ma Ye Chen Jing Li He-Ping Qing Ji-De Wang YaLi Zhang Yang Bai Bei-Guo Long | 2010 | World Journal of Gastroenterology2010，16，44： | 34 |
| 8 | SQUAMOSA Promoter-Binding Protein-Like Transcription Factors:Star Players for Plant Growth and Development显示文摘SQUAMOSA Promoter-Binding Protein-Like (SPL) genes encode plantspecific transcription factors that play important roles in plant phase transition,flower and fruit development,plant architecture,gibberellins signaling,sporogenesis,and response to copper and fungal toxins.In Arabidopsis,many SPL genes are post-transcriptionally regulated by the microRNA (miRNA) miR156,among which AtSPL9 in turn positively regulates the expression of the second miRNA miR172.This miR156AtSPL9-miR172 regulatory pathway plays critical roles during juvenile to adult leaf development and the miR156-SPLs feedback interaction persists all through the plant development,which may be conserved in other plants.In the present paper,we provide a concise review on the most recent progress in the regulatory mechanisms associated with plant SPL transcription factors,especially in relation to miRNAs.The potential application of these discoveries in agriculture is briefly discussed. | Xiaobo Chen Zenglin Zhang Danmei Liu Kai Zhang Aili Li Long Mao | 2010 | Journal of Integrative Plant Biology2010，52，11： | 31 |
| 9 | Parameterizing soil organic carbon's impacts on soil porosity and thermal parameters for Eastern Tibet grasslands显示文摘This study investigates the stratification of soil thermal properties induced by soil organic carbon (SOC) and its impacts on the parameterization of the thermal properties. Soil parameters were measured for alpine grassland stations and North China flux stations, with a total of 34 stations and 77 soil profiles. Measured data indicate that the topsoils of alpine grasslands contain high SOC contents than underlying soil layers, which leads to higher soil porosity values and lower thermal conductivity and bulk density values in the topsoils. However, this stratification is not evident at the lowland stations due to low SOC contents. Evaluations against measured data show that three thermal conductivity schemes used in land surface models severely overestimate the values for soils with high SOC content (i.e. topsoils of alpine grassland), but they are better for soils with low SOC content. A new parameterization is then developed to take the impacts of SOC into account. The new one can well estimate the soil thermal conductivity values in both low and high SOC content cases, and therefore, it is a potential candidate of thermal conductivity scheme to be used in land surface models. | CHEN YingYing YANG Kun TANG WenJun QIN Jun ZHAO Long | 2012 | Science China Earth Sciences2012，55，6： | 31 |
| 10 | Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication显示文摘AIM: To screen and investigate the effective g RNAs against hepatitis B virus(HBV) of genotypes A-D.METHODS: A total of 15 g RNAs against HBV of genotypes A-D were designed. Eleven combinations of two above g RNAs(dual-g RNAs) covering the regulatory region of HBV were chosen. The efficiency of each g RNA and 11 dual-g RNAs on the suppression of HBV(genotypes A-D) replication was examined by the measurement of HBV surface antigen(HBs Ag) or e antigen(HBe Ag) in the culture supernatant. The destruction of HBV-expressing vector was examined in Hu H7 cells co-transfected with dual-g RNAs and HBVexpressing vector using polymerase chain reaction(PCR) and sequencing method, and the destruction of ccc DNAwas examined in Hep AD38 cells using KCl precipitation, plasmid-safe ATP-dependent DNase(PSAD) digestion, rolling circle amplification and quantitative PCR combined method. The cytotoxicity of these g RNAs was assessed by a mitochondrial tetrazolium assay.RESULTS: All of g RNAs could significantly reduce HBs Ag or HBe Ag production in the culture supernatant, which was dependent on the region in which g RNA against. All of dual g RNAs could efficiently suppress HBs Ag and/or HBe Ag production for HBV of genotypes A-D, and the efficacy of dual g RNAs in suppressing HBs Ag and/or HBe Ag production was significantly increased when compared to the single g RNA used alone. Furthermore, by PCR direct sequencing we confirmed that these dual g RNAs could specifically destroy HBV expressing template by removing the fragment between the cleavage sites of the two used g RNAs. Most importantly, g RNA-5 and g RNA-12 combination not only could efficiently suppressing HBs Ag and/or HBe Ag production, but also destroy the ccc DNA reservoirs in Hep AD38 cells.CONCLUSION: These results suggested that CRISPR/Cas9 system could efficiently destroy HBV expressing templates(genotypes A-D) without apparent cytotoxicity. It may be a potential approach for eradication of persistent HBV ccc DNA in chronic HBV infection patients. | Jie Wang Zhong-Wei Xu Shuang Liu Rui-Yang Zhang Shan-Long Ding Xiao-Meng Xie Lu Long Xiang-Mei Chen Hui Zhuang Feng-Min Lu | 2015 | World Journal of Gastroenterology2015，21，32： | 29 |
| 11 | ON JENSEN'S INEQUALITY FOR g-EXPECTATION显示文摘Briand et al. gave a counterexample showing that given g, Jensen's inequality for g-expectation usually does not hold in general This paper proves that Jensen's inequality for g-expectation holds in general if and only if the generator g (t, z) is super-homogeneous in z. In particular, g is not necessarily convex in z. | JIANG LONG CHEN ZENGJING School of Mathematics and System Sciences, Shandong University, Jinan 250100, China. Department of Mathematics, China University of Mining and Technology, Xuzhou 221008, Jiangsu,China. E-mail: jianglong@math.sdu.edu.cn School of Mathematics and System Sciences, Shandong University, Jinan 250100, China. | 2004 | Chinese Annals of Mathematics,Series B2004，25，3： | 26 |
| 12 | Features of Sandy Debris Flows of the Yanchang Formation in the Ordos Basin and Its Oil and Gas Exploration Significance显示文摘Sandy debris flow is a new genetic type of sand bodies,which has gained much attention in recent years and its corresponding theory is proved to be a significant improvement and even partial denial to the 'Bouma Sequence' and 'turbidite fan' deep-water sedimentary theories to some point. Oil exploration researchers are highly concerned with sandy debris flows for its key role in controlling oil and gas accumulation processes.In this article,by applying sandy debris flows theory and combining a lot work o... | LI Xiangbo CHEN Qilin LIU Huaqing WAN Yanrong WEI Lihua LIAO Jianbo LONG Liwen | 2011 | Acta Geologica Sinica(English Edition)2011，85，5： | 24 |
| 13 | Mechanical Properties Prediction of the Mechanical Clinching Joints Based on Genetic Algorithm and BP Neural Network显示文摘For optimal design of mechanical clinching steel-aluminum joints, the back propagation(BP) neural network is used to research the mapping relationship between joining technique parameters including sheet thickness, sheet hardness, joint bottom diameter etc., and mechanical properties of shearing and peeling in order to investigate joining technology between various material plates in the steel-aluminum hybrid structure car body.Genetic algorithm(GA) is adopted to optimize the back-propagation neural network connection weights.The training and validating samples are made by the BTM? Tog-L-Loc system with different technologic parameters.The training samples' parameters and the corresponding joints' mechanical properties are supplied to the artificial neural network(ANN) for training.The validating samples' experimental data is used for checking up the prediction outputs.The calculation results show that GA can improve the model's prediction precision and generalization ability of BP neural network.The comparative analysis between the experimental data and the prediction outputs shows that ANN prediction models after training can effectively predict the mechanical properties of mechanical clinching joints and prove the feasibility and reliability of the intelligent neural networks system when used in the mechanical properties prediction of mechanical clinching joints.The prediction results can be used for a reference in the design of mechanical clinching steel-aluminum joints. | LONG Jiangqi LAN Fengchong CHEN Jiqing YU Ping | 2009 | Chinese Journal of Mechanical Engineering2009，22，1： | 22 |
| 14 | Large-scale wind turbine blade design and aerodynamic analysis显示文摘Incorporating controlled elitism and dynamic distance crowding strategies, a modified NSGA-II algorithm based on a fast and genetic non-dominated sorting algorithm is developed with the aim of obtaining a novel multi-objective optimization design algorithm for wind turbine blades. As an example, a high-performance 1.5 MW wind turbine blade, taking maximum annual energy production and minimum blade mass as the optimization objectives, was designed. A 1/16-scale model of this blade was tested in a 12 m × 16 m wind tunnel and the experimental results validated the high performance. Moreover, both the computational fluid dynamics (CFD) method and a free-vortex method (FVM) were applied to calculating the aerodynamic performance, which was consistent with the experimental data. For completeness, the CFD and FVM were used to analyze the wake structure, and good and consistent results were obtained between them. | WANG TongGuang WANG Long ZHONG Wei XU BoFeng CHEN Li | 2012 | Chinese Science Bulletin2012，57，5： | 23 |
| 15 | Analysis of the Results of Two Nationwide Surveys on Clonorchis sinensis Infection in China显示文摘Objective To examine Clonorchis sinensis infection in China and evaluate the effectiveness of efforts to prevent and control it,two nationwide surveys were undertaken in 31 provinces,autonomous regions,and municipalities(PAMs) during 1988-92(the 1990 survey) and during 2001-04(the 2003 survey).Methods During the period 2001-04,two sampling methods were applied.The first method repeated the stratified cluster random sampling used in the 1990 survey;the second method applied two-characteristic stratified cluster random sampling in 27 PAMs-the 2003 endemic area(EA) survey.The Kato-Katz thick smear method was used for the nationwide survey.Results The infection rates of Clonorchis sinensis in the 1990 and 2003 surveys were 0.311% and 0.579%,respectively.The infection rate was 2.40% in the 2003 EA survey,and it was estimated that 12.49 million people in China were infected with Clonorchis sinensis.Conclusion The 2003 survey showed that the standardized infection rate of Clonorchis sinensis increased by 74.85% compared with the 1990 survey.The infection rate in males was higher than in females;the infection rate among people eating raw fish or eating out frequently was higher than among those who did not. | CHEN Ying Dan ZHOU Chang Hai XU Long Qi | 2012 | Biomedical and Environmental Sciences2012，25，2： | 22 |
| 16 | miR-126 enhances the sensitivity of non-small cell lung cancer cells to anticancer agents by targeting vascular endothelial growth factor A显示文摘增加的证据建议 hsa-miR-126 (miR-126 ) 在房间衬里的非小的房间肺癌症(NSCLC ) 是下面调整的, miR-126 的恢复由指向特定的分子损害肿瘤房间增长,移植,侵略,和幸存。这里,我们第一次报导 miR-126 涉及调整 NSCLC 房间的反应到癌症化疗。在有 miR-126 的房间模仿的 transfected A549 或禁止者以后,我们发现 miR-126 的提高的水平显著地与减少的一半被联系 adriamycin ( ADM )和长春新碱的最大的禁止的集中, ADM 的增加的累积,脉管的 endothelial 生长因素 A ( VEGFA )和 multidrug 的下面规定联系抵抗的蛋白质 1 ( MRP1 ),并且 Akt 的 inactivation 发信号的小径。而且,提高了 miR-126 的表示压制了 A549 异种皮移植的生长并且禁止了 VEGFA 和 MRP1 的表示。miR-126 能高效地通过和 VEGFA 3-untranslated 区域的相互作用的下面调整 VEGFA 表示,而 VEGFA 的恢复能部分由 miR-126 稀释 MRP1 的抑制。然而, LY294002,表明小径的 PI3K/Akt 的一个禁止者,减少这效果,建议 miR-126 的那提高的表情通过发信号的 VEGF/PI3K/Akt/MRP1 的否定规定增加了 NSCLC 房间的敏感到 anticancer 代理人小径。 | Xiaolan Zhu Hao Li Lulu Long Lulu Hui Haining Chen Xuefeng Wang Huiling Shen Wenlin Xu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，6： | 23 |
| 17 | Mathematical Modeling and Effect of Various Hot-Air Drying on Mushroom(Lentinus edodes)显示文摘An experimental study was performed to determine the characteristics and drying process of mushroom(Lentinus edodes) by 6 different hot-air drying methods namely isothermal drying,uniform raise drying,non-uniform raise drying,uniform intermittent drying,non-uniform intermittent drying and combined drying.The chemical composition(dry matter,ash,crude protein,crude fat,total sugars,dietary fiber,and energy),color parameters(L,a*,b*,c*,and h0) and rehydration capacities were determined.Among all the experiments,non-uniform intermittent drying reached a better comprehensive results due to the higher chemical composition,better color quality associated with high bright(26.381±5.842),high color tone(73.670±2.975),low chroma(13.349±3.456) as well as the highest rehydration(453.76% weigh of dried body).Nine kinds of classical mathematical model were used to obtained moisture data and the Midili-kucuk model can be described by the drying process with the coefficient(R2 ranged from 0.99790 to 0.99967),chi-square(χ2 ranged from 0.00003 to 0.00019) and root mean square error(RMSE ranged from 0.000486 to 0.0012367). | GUO Xiao-hui XIA Chun-yan TAN Yu-rong CHEN Long MING Jian | 2014 | Journal of Integrative Agriculture2014，13，1： | 22 |
| 18 | Genome Editing and Double-Fluorescence Proteins Enable Robust Maternal Haploid Induction and Identification in Maize显示文摘 | Le Dong Lina Li Changlin Liu Chenxu Liu Shuaifeng Geng Xinhai Li Changling Huang Long Mao Shaojiang Chen Chuanxiao Xie | 2018 | Molecular Plant2018，11，9： | 22 |
| 19 | Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer显示文摘Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene. | PAN DengKe1, ZHANG Li1, ZHOU YanRong2, FENG Chong1, LONG Chuan1, LIU Xiao1, WAN Rong3, ZHANG Jian3, LIN AiXing3, DONG EnQiu4, WANG ShuChen4, XU HouGang4 & CHEN HongXing2 1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2 Beijing Institute of Biotechnology, Beijing 100071, China 3 China Agricultural University, Beijing 100193, China 4 Yutian Breeding Swine Farms, Yutian 064104, China | 2010 | Science China(Life Sciences)2010，53，4： | 21 |
| 20 | Influence of connective tissue growth factor antisense oligonucleotide on angiotensin II-induced epithelial mesenchymal transition in HK2 cells显示文摘瞄准:现在的学习被设计进一步调查结缔组织生长因素反感觉 oligonucleotide 的效果(CTGF -- 作为) 在血管收缩素上, II (Ang II ) 导致了管状的房间上皮的间充质的转变(EMT ) 在试管内。方法:人的近似管状的房间线(HK2 ) 在 Dulbecco 的家被种修改的鹰包含 10% 热的媒介使胎儿的小牛浆液失去活性。在在 24 h 的没有浆液的媒介被放松以后, CTGF 的影响 -- 作为(20 mug/mL ) 在 Ang 上导致 II (0.1 micromol/L ) CTGF mRNA 和蛋白质表示被使用反向的抄写聚合酶链反应和 indirect-immunofluorescence 检验。CTGF 的效果 -- 作为在 Ang 上,导致 II 的细胞的超微结构用一台能送的电子显微镜被观察。alpha 光滑的行动(alpha-SMA ) 的表示是由免疫细胞化学的 assayed。在所有实验,控制组与爬的 oligonucleotide 被对待。结果:Ang II 显著地导致了 CTGF mRNA 和蛋白质的增加的表示,这被显示出(P<0.01,分别地) ,它被处理显著地与 CTGF 废除 -- 作为。在与 Ang II 刺激房间以后,细胞的超微结构显示出间充质的特征。这些效果被 CTGF 部分禁止 -- 作为。Ang II 显著地导致了及时依赖的举止,它是显著地与 CTGF 由处理稀释了的 alpha-SMA 的表示 -- 作为(P<0.01,分别地) 。相反,没有类似的效果在与爬的 oligonucleotide 对待的控制组被观察。结论:在人的近似管状的上皮细胞(PTC ) 的 Ang 导致 II 的 EMT 能被处理与 CTGF 稀释 -- 作为。我们的数据提供 CTGF 可能在 PTC 涉及 Ang 导致 II 的 EMT 的进一步的证据。 | Long CHEN Bi-cheng LIE Xiao-liang ZHANG Jian-dong ZHANG Hong LIU Min-xia LI | 2006 | Acta Pharmacologica Sinica2006，27，8： | 21 |
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