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| 1 | Identification of Orientia tsutsugamushi,spotted fever group and typhus group Rickettsia by duplex and nested PCR methods显示文摘Objective:To identify members of genera of rickettsia and O.tsutsugammhi simultaneously.Methods:Rapid and duplex and nested PCR methods have been established by designing primers based on the conserved regions of heat shock protein GroEL gene.345 mouse viscera samples including liver,spleen and kidney,96 Xenopsylla cheopis and 32 chiggers collected from Hongta areas of Yuxi city,Yunnan province were tested by the new PCR methods.Results:The result of the study showed that the new PCR methods could identify most members of genera -Rickettsia and Orientia simultaneously with 100%specificity and its sensitivity could test one copy per microliter.The results of detection prevalence of rickettsioses in mouse,flea and mites DNA samples showed that the total rickettsia infection rate in mouse was 34.78%(120/345).The total infection rates in R.typhi,O.t Karp and R.sibirica of mouse samples were 28.12%(97/345),19.71%(68/345) and O. 29%(1/345) respectively.Co-infection rates in R.typhi and 0.t Karp of mouse samples were 13.33%(46/ 345).O.t Karp type has been the main epidemic strain in these areas.Conclusion:We concluded that this PCR method could be used to detect multi-genera rickettsia simultaneously.Molecular evidences provided in this and previous studies strongly support that Hongta areas of Yuxi city are a natural focus for typhus and scrub typhus with the common occurrence of their confection. | M-C Luan D-Z Yu L Tang L-J Zhang | 2008 | Asian Pacific Journal of Tropical Medicine2008，1，4： | 10 |
| 2 | Simulation of the Accumulation Process of Biogenic Gas Hydrates in the Shenhu Area of Northern South China Sea显示文摘Objective Previous work has largely discussed the relations between sediment structures and accumulation of gas hydrates in the Shenhu area of South China Sea,but has not documented why the gas hydrates occurred at the seafloor topographic highs.Many gas hydrate exploration examples abroad also indicate that the saturation of | LIAO Jing GONG Jianming Lü Wanjun WU Nengyou YUE Baojing LUAN Xiwu HU Gaowei | 2016 | Acta Geologica Sinica(English Edition)2016，90，6： | 3 |
| 3 | Study on ibuprofen/ montmorillonite intercalation composites as drug release system 显示文摘 | Zheng J P Luan L Wang H Y | 2007 | Applied Clay Science2007，36，4： | 1 |
| 4 | The effects of stabilizers on the bath stability of electroless Ni deposition and the deposit 显示文摘 | CHEONG W J LUAN B L SHOESMITH D W | 2004 | Appl Surf Sci2004，229，14： | 1 |
| 5 | Heuristics for scheduling in a flowshop with multiple processors显示文摘 | BRAH S A LUAN L L | | 0，，01： | 1 |
| 6 | 查看详情显示文摘 | Dai N L Xu B Jiang Z W Peng J G Li H Q Luan H X Yang L Y Li J Y | | 0，，： | 1 |
| 7 | The use of external fixation combined with vacuum sealing drainage to treat open com- minuted fractures of tibia in the Wenchuan earthquake显示文摘 | Liu L Tan G Luan F | 2012 | Int Orthop2012，36，7： | 1 |
| 8 | Finite-time filtering for non-linear stochastic systems with partially known transition jump rates显示文摘 | Luan X L Liu F Shi P | 2010 | IET Control Theory and Applications2010，4，5： | 1 |
| 9 | Preparation and thermal properties of porous heterogeneous composite phase change materials based on molten salts/expanded graphite显示文摘 | ZHONG L M ZHANG X W LUAN Y | 2014 | Solar Enengy2014，107，6： | 1 |
| 10 | Environmental performance testing system for thermostructure materials applied in aeroengines显示文摘 | Zhang L T Cheng L F Luan X G Mei H Xu Y D | 2006 | Key Eng Mat2006，313，： | 1 |
| 11 | Development of a stable,early stage unilateral model of Parkinson's disease in middle-aged rhesus monkeys显示文摘 | Ding F Luan L Ai Y Walton A Gerhardt G A Gash D M Grondin R Zhang Z | | 0，，02： | 1 |
| 12 | Protective coating on Mg AZ91D alloys The effect of electroless nickel(EN)bath stabilizers on corrosion behaviour of Ni-P deposit显示文摘 | CHEONG W J LUAN B L SHOESMITH D W | | 0，，04： | 1 |
| 13 | Structure of severe fever with thrombocytopenia syndrome virus nucleocapsid protein in complex with suramin reveals therapeutic potential显示文摘 | Jiao L Y Ouyang S Y Liang M F Niu F F Shaw N Wu W Ding W Jin C Peng Y Zhu Y P Zhang F S Wang T Li C Zuo X B Luan C H Li D X Liu Z J | 2013 | J Virol2013，87，12： | 1 |
| 14 | Predicting the incidence of hand, foot and mouth disease in Sichuan province, China using the ARIMA model显示文摘 | Liu L Luan RS Yin F | 2016 | Epidemiology and Infection2016，144，1： | 1 |
| 15 | Study on ibuprofen/montmorillonite intercalation composites as drug release system显示文摘 | Zheng J P Luan L Wang H Y | 2007 | Applied Clay Science2007，36，4： | 1 |
| 16 | Inhibition of experimental petiodintitis by a topical boron-based antimicrobial显示文摘 | Luan Q Desta T Chehab L | | 0，，： | 1 |
| 17 | Distribution and ecosystem risk assessment of polycyelic aromatic hydrocarbons in the Lu- an River,China显示文摘 | Cao Z G Liu J L Luan Y | 2010 | Ecotoxicology2010，19，5： | 1 |
| 18 | 显示文摘 | Luan W L Gao L Kawaoka H | 2004 | Ceramic International2004，30，： | 1 |
| 19 | Simultaneous determin'ation of five bioactive constituents in the traditional chinese medicinal preparation refined Xue - Fu - Zhu - Yu - Tang by high - performance liquid chromatography with diode - array UV detection 显示文摘 | Luan L J Mi S Y ttu L | 2006 | Journal of Analytical Chemistry2006，61，2： | 1 |
| 20 | Rapamycin is an effective inhibitor of human renal cancer metastasis 显示文摘 | Fu L Luan R D Vuay K S | 2003 | Kidney Int2003，63，3： | 1 |
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