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| 1 | Coupling Rice with Fish for Sustainable Yields and Soil Fertility in China显示文摘About 30 million hectares of rice are cultivated annually in China(FAOSTAT,2019).Because most rice fields are wellirrigated and suitable for fish living(‘fish’here refers to various aquatic animals like carps,prawns,crabs,soft shell turtles,etc.)(Fernando,1993),many efforts have been made to determine how well rice farming and aquaculture can be coupled as a rice-fish system(RFS),of which the same land and water produces both rice and aquatic products.With the technological guidance of RFSs and political supports from the Ministry of Agriculture and Rural Affairs of China since 2010(Supplemental Table 1),multiple types of large-scale,high-efficiency and technologically intensive RFSs are now being demonstrated in the main rice-growing areas of China(National Fisheries Technology Extension Centre,2019),and in total about 1.2 million hectares of rice fields are under RFSs. | GUO Liang HU Liangliang ZHAO Lufeng SHI Xiaoyu JI Zijun DING Lilian REN Weizheng ZHANG Jian TANG Jianjun CHEN Xin | 2020 | Rice science2020，27，3： | 11 |
| 2 | Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution显示文摘The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits,which is of fundamental importance for neuroscience research.Here,we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout(VISo R)for high-throughput,high-quality brain mapping.Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion,the VISo R system effectively eliminates motion blur to obtain undistorted images.By continuously imaging moving samples without stopping,the system achieves high-speed 3D image acquisition of an entire mouse brain within1.5 hours,at a resolution capable of visualizing synaptic spines.A pipeline is developed for sample preparation,imaging,3D image reconstruction and quantification.Our approach is compatible with immunofluorescence methods,enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains.Using this system,we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-sh EGFP mice under resting or forced swimming conditions.Our results indicate the involvement of multiple subcortical areas in stress response.Intriguingly,neuronal activation in these areas exhibits striking individual variability among different animals,suggesting the necessity of sufficient cohort size for such studies. | Hao Wang Qingyuan Zhu Lufeng Ding Yan Shen Chao-Yu Yang Fang Xu Chang Shu Yujie Guo Zhiwei Xiong Qinghong Shan Fan Jia Peng Su Qian-Ru Yang Bing Li Yuxiao Cheng Xiaobin He Xi Chen Feng Wu Jiang-Ning Zhou Fuqiang Xu Hua Han Pak-Ming Lau Guo-Qiang Bi | 2019 | National Science Review2019，6，5： | 7 |
| 3 | Rapid determination of thiamine, riboflavin, niacinamide, pantothenic acid, pyridoxine, folic acid and ascorbic acid in Vitamins with Minerals Tablets by high-performance liquid chromatography with diode array detector显示文摘 | Pengfei Jin Lufeng Xia Zheng Li Ning Che Ding Zou Xin Hu | 2012 | Journal of Pharmaceutical and Biomedical Analysis2012，，： | 1 |
| 4 | Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes显示文摘To investigate the circuit-level neural mechanisms of behavior,simultaneous imaging of neuronal activity in multiple cortical and subcortical regions is highly desired.Miniature head-mounted microscopes offer the capability of calcium imaging in freely behaving animals.However,implanting multiple microscopes on a mouse brain remains challenging due to space constraints and the cumbersome weight of the equipment.Here,we present TINIscope,a Tightly Integrated Neuronal Imaging microscope optimized for electronic and opto-mechanical design.With its compact and lightweight design of 0.43 g,TINIscope enables unprecedented simultaneous imaging of behavior-relevant activity in up to four brain regions in mice.Proof-of-concept experiments with TINIscope recorded over 1000 neurons in four hippocampal subregions and revealed concurrent activity patterns spanning across these regions.Moreover,we explored potential multi-modal experimental designs by integrating additional modules for optogenetics,electrical stimulation or local field potential recordings.Overall,TINIscope represents a timely and indispensable tool for studying the brain-wide interregional coordination that underlies unrestrained behaviors. | Feng Xue Fei Li Ke-ming Zhang Lufeng Ding Yang Wang Xingtao Zhao Fang Xu Danke Zhang Mingzhai Sun Pak-Ming Lau Qingyuan Zhu Pengcheng Zhou Guo-Qiang Bi | 2024 | National Science Review2024，11，1： | 0 |
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