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| 1 | An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita显示文摘Acorn squash(Cucurbita pepo)is an iconic fall vegetable in the United States,known for its unique fruit shape and also prized for its culinary properties.Little is known about the metabolism that underlies the development of fruit quality attributes such as color,sweetness,texture and nutritional qualities in acorn squash,or any other winter squash grown worldwide.To provide insight into winter squash fruit and seed development and add to the genomic resources in the Cucurbita genus,RNA sequencing was used to generate an acorn squash fruit and seed transcriptome from the cultivar Sweet REBA at critical points throughout fruit development.141838600 high-quality paired-end Illumina reads were assembled into 55949 unigenes.85%of unigenes with predicted open reading frames had homology with previously identified genes and over 62%could be functionally annotated.Comparison with the watermelon and cucumber genomes provided confirmation that the unigenes are full-length and comprehensive,covering an average of 90%of the coding sequence of their homologs and 72%of the cucumber and watermelon exomes.Key candidate genes associated with carotenoid and carbohydrate metabolism were identified toward a resource for winter squash fruit quality trait dissection.This transcriptome represents a major advance in C.pepo genomics,providing significant new sequence information and revealing the repertoire of genes expressed throughout winter squash fruit and seed development.Future studies on the genetic basis of fruit quality and future breeding efforts will be enhanced by tools and insights developed from this resource. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2015 | Horticulture Research2015，2，1： | 11 |
| 2 | Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin显示文摘Both the fruit mesocarp and the seeds of winter squash can be used for consumption,although the focus of breeding efforts varies by cultivar.Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content,percent dry matter,and percent soluble solids,while these traits are essentially ignored in oilseed pumpkins.To compare fruit development in these two types of squash,we sequenced the fruit transcriptome of two cultivars bred for different purposes:an acorn squash,‘Sweet REBA’,and an oilseed pumpkin,‘Lady Godiva’.Putative metabolic pathways were developed for carotenoid,starch,and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways.Gene expression,especially of known rate-limiting and branch point genes,corresponded with metabolite accumulation both across development and between the two cultivars.Thus,developmental regulation of metabolite genes is an important factor in winter squash fruit quality. | Lindsay E Wyatt Susan R Strickler Lukas A Mueller Michael Mazourek | 2016 | Horticulture Research2016，3，1： | 7 |
| 3 | Antioxidant treatment with N-acet ylcyst eine and vitamin C in patients with chronic bronchitis显示文摘 | Lukas R Scha rling B Sehultze Weminghaus G | 2005 | Dt sch Med Wochensehr2005，130，11： | 1 |
| 4 | Hemiarthroplasty for man - agement of proximal humeral fractures 显示文摘 | Taller S Krivohlavek M Lukas R | 2007 | Acta Chir Orthop TraumatolCeeh2007，74，： | 1 |
| 5 | Who cooperates on R&D? 显示文摘 | Fritsch M Lukas R | 2001 | Research Policy2001，30，2： | 1 |
| 6 | AMH/MIS:what we know already about the gene,the protein and its regulation显示文摘 | Rey R Lukas C Lasala C | 2003 | Mol Cell Endocrinol2003，211，： | 1 |
| 7 | The mixed layer of the western equatorial Pacific Ocean 显示文摘 | Lukas R Lindstrom E | 1991 | J Geophys Res1991，96，： | 1 |
| 8 | CT-guided internal fixation of a hannah's fracture显示文摘 | Taller S Suehomel P Lukas R | | 0，，： | 1 |
| 9 | Fertility-sparing surgery in patients with cervical Cancer显示文摘 | Lukas R Petr S Helena R | | 0，，02： | 1 |
| 10 | Catalysis and function of the p38 alpha MK2a signaling complex显示文摘 | Lukas S M Kroe R R Wildeson J | 2004 | Biochemistry2004，43，31： | 1 |
| 11 | Hemiarthroplasty for management of proximal humeral fractures显示文摘 | Taller S Krivohlavek M Lukas R | 2007 | Aeta Clair Orthop Traumatol Cech2007，74，4： | 1 |
| 12 | EULAR recommendations for the -management of early arthritis:report of a task force of the European Standing Committee for International Clinical Studies Including Therapeutics(ESCISIT)显示文摘 | Combe B Landewe R Lukas C | | 0，，： | 1 |
| 13 | The interface or air-sea flux component of the TOGA Coupled Ocean-Atmosphere Response Experiment and its Impact on Future Air-Sea Interaction Studies显示文摘 | WELLER R A BRADLEY E F LUKAS R | 2004 | J Atmos Ocean Tech2004，21，： | 1 |
| 14 | CT-guided internal fixation of a Hangman's fracture显示文摘 | Taller S Suchomel P Lukas R | 2000 | Euro Spine J2000，9，： | 1 |
| 15 | CT guided inter- nal fixation of a Hangman's fracture显示文摘 | Taller S Suchomel P Lukas R | 2000 | gur Spine J2000，，： | 1 |
| 16 | Asymmetric Cyclization of 2' -Hydroxychalcones to Flavanones: Catalysis by Ch-Iral Bronsted Acids and Bases显示文摘 | CLAUDIA D GERHARD R LUKAS H | 2007 | Eur J Org Chem2007，，1： | 1 |
| 17 | Current status of the nomenclature for nicotinic acetylcholine receptors and their subunits显示文摘 | LUKAS R J CHANGEUX J P NOVERE N L | 1999 | Pharmacol Rev1999，51，： | 1 |
| 18 | Surgical treatment of fractures of the odontoid process显示文摘 | Suchomel P Taller S Lukas R | 2000 | Rozhl Chir2000，79，7： | 1 |
| 19 | The mixed layer of western equatorial Pacific Ocean显示文摘 | LUKAS R LINDSTROM E | 1991 | Journal of Geophysical Research1991，96，： | 1 |
| 20 | Widely tunable torsional optical filter显示文摘 | CARLOS F R MATEUS C H C LUKAS C | 2002 | IEEE Photon Technol Lett2002，14，6： | 1 |
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