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| 1 | Isolation and characterization of facultative mixotrophic ammonia-oxidizing bacteria from constructed wetlands显示文摘Autotrophic ammonia-oxidizing bacteria (AOB) have been widely studied in constructed wetlands systems,while mixotrophic AOB have been less thoroughly examined.Heterotrophic bacteria were isolated from wastewater and rhizospheres of macrophytes of constructed wetlands,and then cultivated in a mixotrophic medium containing ammonium and acetic acid.A molecular characterization was accomplished using ITS-PCR amplification,and phylogenetic analysis based on 16S rRNA gene sequences.Results showed the presence of 35 bacteria,among 400 initially heterotrophic isolates,that were able to remove ammonia.These 35 isolates were classified into 10 genetically different groups based on ITS pattern.Then,a collection of 10 isolates were selected because of their relatively high ammonia removal efficiencies (ARE 80%) and their phylogenetic diversity.In conditions of mixotrophy,these strains were shown to be able to grow (increase of optical density OD 660 during incubation with assimilation of nitrogen into cellular biomass) and to oxidize ammonia (important ammonia oxidation efficiencies,AOE between 79% and 87%).Among these facultative mixotrophic AOB,four isolates were genetically related to Firmicutes (Bacillus and Exiguobacterium),three isolates were affiliated to Actinobacteria (Arthrobacter) and three other isolates were associated with Proteobacteria (Pseudomonas,Ochrobactrum and Bordetella). | Soulwène Kouki Neila Saidi Fadhel M'hiri Houda Nasr Hanène Cherif Hadda Ouzari Abdennaceur Hassen | 2011 | Journal of Environmental Sciences2011，23，10： | 9 |
| 2 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 3 | Non-Smad signaling pathways显示文摘 | Yabing Mu Shyam Kumar Gudey Maréne Landstr?m | 2012 | Cell and Tissue Research2012，，1： | 3 |
| 4 | Juvenile ferric iron prevents microbiota dysbiosis and colitis in adult rodents显示文摘AIM: To assess whether juvenile chronic ferric iron ingestion limit colitis and dysbiosis at adulthood in rats and mice. METHODS: Two sets of experiments were designed. In the first set, recently weaned mice were either orally administered ferrous (Fe2+) iron salt or ferric (Fe3+) microencapsulated iron for 6 wk. The last week of experiments trinitrobenzene sulfonic acid (TNBS) colitis was induced. In the second set, juvenile rats received the microencapsulated ferric iron for 6 wk and were also submitted to TNBS colitis during the last week of experiments. In both sets of experiments, animals were sacrificed 7 d after TNBS instillation. Severity of the inflammation was assessed by scoring macroscopic lesions and quantifying colonic myeloperoxidase (MPO) activity. Alteration of the microflora profile was estimated usingquantitative polymerase chain reaction (qPCR) by measuring the evolution of total caecal microflora, Bacteroidetes, Firmicutes and enterobacteria. RESULTS: Neither ferrous nor ferric iron daily exposures at the juvenile period result in any effect in control animals at adulthood although ferrous iron repeated administration in infancy limited weight gain. Ferrous iron was unable to limit the experimental colitis (1.71 ± 0.27 MPO U/mg proteinvs 2.47 ± 0.22 MPO U/mg protein in colitic mice). In contrast, ferric iron significantly prevented the increase of MPO activity (1.64 ± 0.14 MPO U/mg protein) in TNBS-induced colitis. Moreover, this positive effect was observed at both the doses of ferric iron used (75 and 150 mg/kg per day po - 6 wk). In the study we also compared, in both rats and mice, the consequences of chronic repeated low level exposure to ferric iron (75 mg/kg per day po - 6 wk) on TNBS-induced colitis and its related dysbiosis. We confirmed that ferric iron limited the TNBS-induced increase of MPO activity in both the rodent species. Furthermore, we assessed the ferric iron incidence on TNBS-induced intestinal microbiota dysbiosis. At first, we needed to optimize the isolation and quantify DNA copy numbers using standard curves to perform by qPCR this interspecies comparison. Using this approach, we determined that total microflora was similar in control rats and mice and was mainly composed of Firmicutes and Bacteroidetes at a ratio of 10/1. Ferric juvenile administration did not modify the microflora profile in control animals. Total microflora numbers remained unchanged whichever experimental conditions studied. Following TNBS-induced colitis, the Firmicutes/Bacteroidetes ratio was altered resulting in a decrease of the Firmicutes numbers and an increase of the Bacteroidetes numbers typical of a gut inflammatory reaction. In parallel, the subdominant population, the enterobacteria was also increased. However, ferric iron supplementation for the juvenile period prevented the increase of Bacteroidetes and of enterobacteria numbers consecutive to the colitis in both the studied species at adulthood.CONCLUSION: Rats and mice juvenile chronic ferric iron ingestion prevents colitis and dysbiosis at adulthood as assessed by the first interspecies comparison. | Chourouk Ettreiki Pascale Gadonna-Widehem Irène Mangin Mose Coёffier Carine Delayre-Orthez Pauline M Anton | 2012 | World Journal of Gastroenterology2012，18，21： | 3 |
| 5 | Low dose airborne endotoxin exposure enhances bronchial responsiveness to inhaled allergen in atopic asthmatics 显示文摘 | Boehlecke B Hazucha M Alexis NE | 2003 | J Allergy Clin Immunol2003，112，： | 1 |
| 6 | E-cadherin mediates adhesion and suppresses cell motility via distinct mechanisms显示文摘 | Chen H Paradies NE Fedor-Chaiken M | 1997 | J Cell Sci1997，110，3： | 1 |
| 7 | Adipocytokines and aging:adiponectin and leptin显示文摘 | Gulcelik NE Halil M Ariogul S | 2013 | Minerva Endocrinol2013，38，2： | 1 |
| 8 | Azithromycin for the secondary prevention of coronary heart disease events:The WIZARD study:a randomized controlled trial显示文摘 | O'Con nor CM Dun ne M W Pfeffer M A | | 0，，： | 1 |
| 9 | Smoke exposure interacts with Adam33 polymorphisms in the development of lung function and hyperresponsiveness显示文摘 | Reijmerink NE Kerkhof M Koppelman GH | | 0，，06： | 1 |
| 10 | Peroxisome proliferator-activated receptor gamma contributes to T lymphocyte apoptosis during sepsis显示文摘 | Soller M Tautenhahn A Brüne B | 2006 | Leukocyte Biol2006，79，1： | 1 |
| 11 | The management of psychiatric emerencies显示文摘 | Mavrogiorgou ou P Bru ne M Juckel G | 2011 | Dtsch Arztebl Int2011，108，13： | 1 |
| 12 | Anti-CCR7 monoclonal antibodies as a novel tool for the treatment of chronic lymphocyte leukemia显示文摘 | Alfonso-Perez M Lopez-Giral S Quintana NE | 2006 | J Leu- koc Biol2006，79，6： | 1 |
| 13 | Complete destruction of a tissue-engineered porcine xenograft in pulmonary valve position after the Ross procedure显示文摘 | Hiemarm NE Mani M Huebler M | 2010 | J Thorac Cardiovasc Surg2010，139，4： | 1 |
| 14 | Mevastatin, an HMG-CoA reductase inhibitor, reduces stroke damage and upregulates endothelial nitric oxide synthase in mice显示文摘 | Amin-Hanjani S Stagliano NE Yamada M | 2001 | Stroke2001，32，4： | 1 |
| 15 | Worm proteins of schistosoma mansoni reduce the severity of experimental chro- nic colitis in mice by suppressing colonic proinflammatory im- mune responses显示文摘 | Heylen M Ruyssers NE De Man JG | 2014 | PLoS One2014，9，10： | 1 |
| 16 | A new intraocular lens design to reduce spheri- cal aberration of pseudophakic eyes 显示文摘 | HOLLADAYJT PIERSPA KORANYI G VAN DER MOOREN M NORRBY NE | 2002 | J Refract Surg2002，18，6： | 1 |
| 17 | The membrane-proximal external region of the human immunodeficiency virus type 1 envelope:dominant site of antibody neutralization and target for vaccine design显示文摘 | Montero M Houten NE Wang X | 2008 | Microbiol Mol Biol Rev2008，72，1： | 1 |
| 18 | Carbon fiber scaffolds in the treat- ment of early knee osteoarthritis显示文摘 | Brittberg M Fan NE Peterson L | 1994 | Clinical Orthopaedics and Re- lated Research1994，37，： | 1 |
| 19 | Atherosclerosis and Cancer: Common molecular pathways of disease development and progression 显示文摘 | Ross JS Stagliano NE Donoran M J | 2001 | Ann NY Acad Sci2001，947，12： | 1 |
| 20 | Simultaneous spectrophotometric determination of amylose and amylopectin in starch from maize kernel by multi-wavelength analysis显示文摘 | Séne M Thévenot C Prioul J L | | 0，，02： | 1 |
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