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| 1 | 造血微环境决定脐血造血干/祖细胞的早期分裂行为显示文摘目的 在特定生长因子的培养体系中 ,观察模拟造血微环境及粘附因素对人类脐血造血干 祖细胞早期分裂行为的影响。方法 ①应用流式细胞仪 (FACS)分选CD3 4+CD3 8- 细胞 ;②细胞培养体系中应用干细胞支持性基质AFT0 2 4及单一粘附因素纤维结合素 (Fn) ,并观察其对细胞早期分裂行为的影响。结果 ①在联合生长因子的条件下 ,人类脐血CD3 4+CD3 8- 细胞早期分裂呈现固定比例的静止、慢分裂、快分裂以及不对称分裂状态 ;②未观察到单一粘附因素Fn对其早期分裂行为的影响 ;③干细胞支持性基质AFT0 2 4所模拟的体外造血微环境可促使脐血造血干 祖细胞广泛增殖并更多地进行不对称分裂。结论 ①CD3 4+CD3 8- 细胞群体具有异质性 ,由具有不同增殖行为的亚群组成 ,在体外培养早期存在不对称分裂 ;②体外模拟的骨髓微环境AFT0 2 4基质滋养层较细胞因子及单一粘附因素能够更好地支持造血干 | 刘代红 M Punzel AD Ho | 2002 | 中华血液学杂志2002，23，10： | 4 |
| 2 | Effects of L carnitine on mechanical isolated rat hearts in relation recovery ofand pakni tats显示文摘 | Loster H Punzel M | 1998 | CturlekMof Cell to the perfusion with glucose1998，185，： | 1 |
| 3 | Prolonged oral L-carnitine substitution increases bicycle ergometer performance in patients with severe, ischemically induced cardiac insufficiency 显示文摘 | Losterh Miehe K Punzel M | 1999 | Cardiovasc Drugs Ther1999，13，6： | 1 |
| 4 | Effects of L-carnitine on mechanical recovery of isolated rat hearts in relation to the perfusion with glucose and palmitate显示文摘 | LOSTER H PUNZEL M | 1998 | Mol Cell Biochem1998，185，12： | 1 |
| 5 | Effects of L camitine on mechanical recovery of isolated rat hearts in relation to the perfusion with glucose and palmitate显示文摘 | Loster H Punzel M | 1998 | Mol Cell Biochem1998，185，： | 1 |
| 6 | Effects of L- camitine on mechanical recovery of isolated rat hearts in relations to the perfusion with gluces and palmirate 显示文摘 | Loster H Punzel M | 1998 | Mol Cell Biochem1998，185，12： | 1 |
| 7 | The symmetry of initial divisions of human hematopoietic progenitors is altered only by the cellular microenvironment显示文摘 | Punzel M Liu DH Zhang T | 2003 | Exp Hematol2003，31，4： | 1 |
| 8 | Prolonged oral L - camitine substituteion increases bicycle ergometer performance in patients With severe, ischemically induced cardicac insufficiency 显示文摘 | Loster H Miehe K Punzel M | 1999 | Cardiovasc Drugs Ther1999，13，6： | 1 |
| 9 | Prolonged oral L - carnitine substitution increases bicycle ergometer performance in patients with severe,ischemically induced cardiac insufficiency显示文摘 | Losterh Miehe K Punzel M | 1999 | Cardiovasc Drugs Ther1999，13，6： | 1 |
| 10 | Prolonged oral L-carnitine substitution increases bicycle ergometer performance in patients with severe, ischemically induced cardiac insufficiency 显示文摘 | LOSTER H MIEHE K PUNZEL M | 1999 | Cardiovasc Drugs Ther1999，13，6： | 1 |
| 11 | The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro显示文摘 | Punzel M Wissink SD Miller JS | 1999 | Blood1999，93，11： | 1 |
| 12 | Single adult hu- man CD34 /Lin-/CD38- progenitors give rise to natural killer cells, B-lineage cells, dendritic cells, and myeloid cells显示文摘 | Miller JS Mccullar V Punzel M | 1999 | Blood1999，93，1： | 1 |
| 13 | The myeloid- lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro 显示文摘 | Punzel M Wissink SD Miller JS | 1999 | Blood1999，93，11： | 1 |
| 14 | Effects of L-camitine on mechanical recovery of isolated rat hearts in relation to the perfusion with glucose and palmitate显示文摘 | Loster H Punzel M | 1998 | Mol Cell Biochem1998，185，12： | 1 |
| 15 | Prolonged oval L-camitine substitution increases bicycle ergometer perfonnance in patients with severe, ischemically induced cardiac insufficiency显示文摘 | Loster H Miehe K Punzel M | 1999 | Cardiovasc Drugs Ther1999，13，6： | 1 |
| 16 | Divisional history and pluripoteey of human hematopoietic stem cells显示文摘 | Punzel M Ho AD | 2001 | Ann N Y Acad Sci2001，938，： | 1 |
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