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| 1 | Vehicle-bridge coupled vibrations in different types of cable stayed bridges显示文摘系统和呆在的电报的不同类型的效果在联合颤动回答上衔接的车辆桥的联合颤动的数字分析用 ANSYS 在这份报纸被介绍了。桥模型和车辆模型独立地被造它没在 ANSYS 有内部关系。车辆桥联合了颤动关系被使用随后在数字分析期间在车辆和桥模型上强加了的 APDL 程序获得。建议模型通过一块地被验证大小和文学数据。电报的车辆桥回声(联合颤动) 的判定的方法,可能性,和标准在交通流动下面呆在桥(漂浮的系统和半漂浮的系统) 被介绍。结果显示在车辆之间的间隔时间是在条件下面的回声刺激频率上的主要影响因素同等地, spaced 交通流动。把呆在的桥比作电缆线的另外的类型,漂浮的桥系统有相对高的可能性到原因车辆桥回声。 | Lingbo WANG Peiwen JIANG Zhentao HUI Yinping MA Kai LIU Xin KANG | 2016 | Frontiers of Structural and Civil Engineering2016，10，1： | 2 |
| 2 | Extracellular-superoxide dismutase DNA methylation promotes oxidative stress in homocysteine-induced atherosclerosis显示文摘In the present study,we investigate the effect of homocysteine(Hcy)on extracellular-superoxide dismutase(ECSOD)DNA methylation in the aorta of mice,and explore the underlying mechanism in macrophages,trying to identify the key targets of Hcy-induced EC-SOD methylation changes.ApoE--mice are fed different diets for 15 weeks,EC-SODand DNAmethyltransferase1(DNMT1)expression levels aredetected byRT-PCRandwestern blot analysis.EC-SOD methylation levels are assessed by ntMS-PCR.After EC-SOD overexpression or knockdown in macrophages,following the transfection of macrophages with pEGFP-N1-DNMT1,the methylation levels of ECSOD are detected.Our data show that the concentrations of Hcy and the area of atherogenic lesions are significantly increased in ApoE--mice fed with a high-methionine diet,and have a positive correlation with the levels of superoxide anions,which indicates that Hcy-activated superoxide anions enhance the development of atherogenic lesions.EC-SOD expression is suppressed by Hcy,and the content of superoxide anion is increased when EC-SOD is silenced by RNAi in macrophages,suggesting that EC-SOD plays a major part in oxidative stress induced by Hcy.Furthermore,the promoter activity of EC-SOD is increased following transfection with the-1/-110o fragment,and EC-SOD methylation level is significantly suppressed by Hcy,and more significantly decreased upon DNMT1 overexpression.In conclusion,Hcy may alter the DNA methylation status and DNMT1 acts as the essential enzyme in the methyl transfer process to disturb the status of EC-SOD DNA methylation,leading to decreased expression of Ec-sODandincreasedoxidativestressandatherosclerosis. | Shengchao Ma Guanjun Lu Qing Zhang Ning Ding Yuzhen Jie Hui Zhang Lingbo Xu Lin Xie Xiaoling Yang Huiping Zhang Yideng Jiang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，9： | 1 |
| 3 | Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification显示文摘A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A.minutum,which can produce paralytic shellfish poisoning (PSP).Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA.The method worked well in less than an hour under isothermal conditions of 65 C.LAMP specificity was validated in closely related algae as a comparison,suggesting the strict specificity of the LAMP primers.Two visual inspection approaches were feasible to interpret the positive or negative results.The detection limits of A.catenella and A.minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA,respectively.The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae.These characteristics of species specificity,sensitivity,and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A.catenella and A.minutum. | ZHANG Fengying SHI Yanhong JIANG Keji XU Zhaoli MA Lingbo | 2012 | Acta Oceanologica Sinica2012，31，2： | 1 |
| 4 | Electrochemical analysis of microRNAs with hybridization chain reaction-based triple signal amplification显示文摘Selective and sensitive detection of trace microRNA is important for early diagnosis of diseases due to its expression level related to diseases.Herein,a triple signal amplification strategy is developed for trace microRNA-21 (miRNA-21) detection by combining with target-triggered cyclic strand displacement reaction (TCSDR),hybridization chain reaction (HCR) and enzyme catalytic amplification.Four DNA hairpins(H1,H2,H3,H4) are employed to form an ultralong double-strand DNA (dsDNA) structure,which is initiated by target miRNA-21.As H3 and H4 are labeled with horseradish peroxidase (HRP),numerous HRPs are loaded on the long dsDNA,producing significantly enhanced electrocatalytic signals in the hydrogen peroxide (H_(2)O_(2)) and 3,3,5,5-tetramethylbenzidine (TMB) reaction strategy.Compared with single signal amplification,the triple signal amplification strategy shows higher electrochemical response,wider dynamic range and lower detection limit for miRNA-21 detection with excellent selectivity,reproducibility and stability.Taking advantage of the triple signal amplification strategy,the proposed electrochemical biosensor can detect miRNA-21 in 10 He La cell lysates,suggesting that it is a promising method for fruitful assay in clinical diagnosis. | Jianfeng Ma Lingbo Gong Yingying Cen Lin Feng Yan Su Xingfen Liu Jie Chao Ying Wan Shao Su Lianhui Wang | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
| 5 | The Novel Hatchery Facilities Based on Main Effect Factors of Seedling Rearing of Mud Crab (Scylla spp.) in China显示文摘 | Zhenguo Qiao Jiangang Wang Zhongli Yux Keji Jiang Lingbo Ma | 2010 | Journal of Life Sciences2010，4，3： | 0 |
| 6 | The Taxonomic Status of Gymnura bimaculata and G. japonica: Evidence from Mitochondrial DNA Sequences显示文摘 | Anglv Shen Chunyan Ma Yong Ni Zhaoli Xu Lingbo Ma | 2012 | Journal of Life Sciences2012，6，1： | 0 |
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