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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Effect of magnitude of vertical whole-body vibration on absorbed power for the seated human body显示文摘 | MANSFIELD N J GRIFFIN M J | 1998 | Journal of Sound and Vibration1998，215，4： | 1 |
| 3 | Monte carlo studies of dendrimer macromolecule显示文摘 | MANSFIELD M L KLUSHINL Z | 1993 | Macromolecule1993，26，16： | 1 |
| 4 | Self-reported musculoskeletal problems amongst professional truck drivers 显示文摘 | ROBB M J MANSFIELD N J | 2007 | Ergonomics2007，50，6： | 1 |
| 5 | Trypanosomes Expressing a Mosaic Variant Surface Glycopmtein Coat Escape Early Detection by the Immune System显示文摘 | Dubois M E Demick K P Mansfield J M | 2005 | Infection Immunity2005，73，5： | 1 |
| 6 | Construction of internal cDNA competitors for mearuring IL-10 and IL-12 cytokine gene expression in swine显示文摘 | Urban J F Murtaugh M P | 1998 | Vet Immunol Immunopathol1998，65，： | 1 |
| 7 | 89Sr for palliation of bone metastases显示文摘 | Raddy E K Robinson R G Mansfield C M | 1986 | J Natl Med Assoc1986，78，11： | 1 |
| 8 | Early events in host-pathogen interactions显示文摘 | Grant M Mansfield J | 1999 | Curr Opinion Plant Biol1999，2，： | 1 |
| 9 | Construction of internal cDNA competitors for mearuring ID 10 and IL-12 cytokine gene expression in swine显示文摘 | Mansfield L S Urban J F Murtaugh M P | 1998 | Vet Immunol Immuno-Pathol1998，65，： | 1 |
| 10 | Metformin has direct effects on human ovarian steroidogenesis显示文摘 | Mansfield R Galea R Brincat M | 2003 | Fertil Steril2003，79，4： | 1 |
| 11 | Detection of Schmallenberg virus serum neutralising antibodies显示文摘 | Mansfield K L Rocca S A Khatri M | 2013 | J Virol Methods2013，188，： | 1 |
| 12 | Fault growth by segment linkage: An explanation for scat- ter in maximum displacement and trace length data for the Canyonlands Grabens of SE Utah 显示文摘 | Cartwright J A Trudgill B D Mansfield C M | 1995 | Journal of Structural Geology1995，17，： | 1 |
| 13 | High refractory, low misfit Ru-containing single-crystal superalloys显示文摘 | CARROLL L J FENG Q MANSFIELD J F POLLOCK T M | 2006 | Metallurgical and Materials Transactions A2006，37，10： | 1 |
| 14 | Changes in stomatal behavior and guard cell cytosolic free calcium in response to oxidative stress 显示文摘 | McAinsh M R Clayton H Mansfield T A | 1996 | Plant Physiology1996，111，： | 1 |
| 15 | Imitation Costs and Patents: An Empirical Study 显示文摘 | Mansfield E M Schwartz S Wagner | 1981 | The Economic Journal1981，91，364： | 1 |
| 16 | Soluble P-selectin levels,P-selectin polymorphisms and cardiovascular disease显示文摘 | Carter A M Anagnostopoulou K Mansfield MW | 2003 | Thromb Haemost2003，1，： | 1 |
| 17 | Soluble P-selectin levels,P-selectin polymorphisms and cardiovascular disease显示文摘 | Carter A M Anagnostopoulou K Mansfield MW | 2003 | Thromb Haemost2003，1，： | 1 |
| 18 | Effects of combination anti-vascular endothelial growth factor and anti-epidemal growth factor therapies on the growth of gastric cancer in a nude mouse model显示文摘 | Jung YD Mansfield PF Akagi M | 2002 | Eur J Cancer2002，38，： | 1 |
| 19 | Foreign Trade and US Research and Devel-opment 显示文摘 | MANSFIELD M | 1979 | Review of Economic and Statistics1979，，3： | 1 |
| 20 | The RNase Ill enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb显示文摘 | Harfe B D McManus M T Mansfield J H | 2005 | Proc Natl Acad Sci USA2005，102，10： | 1 |
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