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| 1 | Morphological, thermal, and mechanical characteristics of polymer/layered silicate nanoeomposites : The role of fill- er modification level 显示文摘 | MARRAS S I TSIMPLIARAKI A ZUBURTIKUDIS I | 2009 | Poly Eng Sci2009，49，6： | 1 |
| 2 | Porous poly (L-lactic acid ) nanocomposite scaffolds prepared by phase inversion using supercritical CO2 as antisolvent显示文摘 | Tsivintzelis J Marras S I Zuburtikudis J | 2007 | Polymer2007，48，21： | 1 |
| 3 | Fatigue-crack-propagation thresholds in a nickel-base superalloy at high frequencies and temperatures显示文摘 | SHYAM A PADULA S A MARRAS S I MILLIGAN W W | 2002 | Metallurgical and Materials Transactions A2002，33，： | 1 |
| 4 | 查看详情显示文摘 | Tsimpliaraki A Tsivintzelis I Marras S I Zuburtikudis I Panayiotou C | | 0，，03： | 1 |
| 5 | The Effect of Surface Chemistry and Nanoclay Loading on the Microcellular Structure of Porous Poly (d, l-lactic acid) Nanocomposites显示文摘 | Tsimpliaraki A Tsivintzelis I Marras S I | 2011 | J of Supercrit Fluid2011，57，： | 1 |
| 6 | The Effect of Surface Chemistry and Nanoclay Loading on the Microcellular Structure of Porous Poly(d, 1 lactic acid) Nanocomposites显示文摘 | Tsimpliaraki A Tsivintzelis I Marras I S | 2011 | J of Supercritical Fluids2011，57，： | 1 |
| 7 | Thermal and colloidal behavior of amine-treated clays:The role of amphiphilic organic cation concentration显示文摘 | Marras S I Tsimpliaraki A Zuburtikudis I | 2007 | Journal of Colloid and Interface Science2007，315，2： | 1 |
| 8 | Ocean color variability in the Indonesian Seas during the SeaWiFS era显示文摘 | Susanto R D Moore T S I Marra J | 2006 | Geochem Geophys Geosyst2006，7，05： | 1 |
| 9 | The genome sequence of the SARS-associated coranavirus 显示文摘 | I MARRA M JONES S ASTELL C R | 2003 | Science2003，300，： | 1 |
| 10 | Porous poly(1- lactid acid)nano composite scaffolds preared by phase inver- sion using supercrtial CO2 as antisolent显示文摘 | Tsvntzelis I Marras S I Zburtikudis I | 2007 | Polymer2007，48，21： | 1 |
| 11 | Study of the three-way interaction between Trichoderma atroviride,plant and fungal pathogens by using a proteomic approach显示文摘 | MARRA R AMBROSINA P CARBONE V VINALE F WOO S L RUOCCO M CILIENTO R LANZUISE S FERRAIOLI S SORIENTE I GIGANTE S TURRA D FOGLIANO V SCALA F LORITO M | 2006 | Current Genetics2006，50，： | 1 |
| 12 | Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences显示文摘 | STRAUSBERG R L FEINGOLD E A GROUSE L H DERGE J G KLAUSNER R D COLLINS F S WAGNER L SHENMEN C M SCHULER G D ALTSCHUL S F ZEEBERG B BUETOW K H SCHAEFER C F BHAT N K HOPKINS R F JORDAN H MOORE T MAX S I WANG J HSIEH F DIATCHENKO L MARUSINAK FARMER A A RUBIN G M HONG L STAPLETON M SOARES M B BONALDO M F CASAVANT T L SCHEETZ T E BROWNSTEIN M J USDIN T B TOSHIYUKI S CARNINCI P PRANGE C RAHA S S LOQUELLANO N A PETERS G J ABRAMSON R D MULLAHY S J BOSAK S A MCEWAN P J MCKERNAN K J MALEK J A GUNARATNE P H RICHARDS S WORLEY K C HALE S GARCIA A M GAY L J HULYK S W VILLALON D K MUZNY D M SODERGREN E J LU X GIBBS R A FAHEY J KETTEMAN M MADAN A RODRIGUES S SANCHEZ A WHITING M YOUNG A C SHEVCHENKO Y BOUFFARD G G BLAKESLEY R W TOUCHMAN J W GREEN E D DICKSON M C RODRIGUEZ A C GRIMWOOD J SCHMUTZ J MYERS R M BUTTERFIELD Y S KRZYWINSKI M I SKALSKA U SMAILUS D E SCHNERCH A SCHEIN J E JONES S J MARRA M A | 2002 | Proc Natl Acad Sci USA2002，99，16： | 1 |
| 13 | Extracellular proteome of Trichoderma harzianum to identify proteins with biotechnological value显示文摘Trichoderma harzianum strain T22 parasitizes and controls many phytopatogenic fungi and is applied commercially as biological control agent. The production of hydrolitic enzymes appears to be a key factor in the parasitic process. We tested the endo-esochitinolitic and glucanolitic activities of culture filtrates of T22 grown under carbon and nitrogen starvation or in presence of biomass or cell walls of the phytopathogenic fungi Botrytis cinerea, Rhizoctonia solani and Pythium ultimum. The highest level of enzimatic activities was found in culture where the mycoparasite interacted with a phytopathogenic fungus. Therefore we used a proteomic approach to investigate changes in the complex mixture of extracellular proteins secreted by T. harzianum strain T22 in order to identify proteins of potential biotechnology value for commercial and industrial use.Proteome technology has greatly enhanced our ability to conduct functional genomics studies. Nevertheless only a few studies have been published so far on the fungal extracellular proteome. Sample preparation remains the most critical step in analyses based on two-dimensional gel electrophoresis (2-DE), and it requires to be optimized for each specific application. In this study, our first aim was to set up the extraction protocol of the extracellular proteins secreted by T . harzianum strain T22 when it was grown in vitro .The secreted proteins were analysed by two-dimensional electrophoresis (2-DE) and substantial changes in the extracellular proteome of the mycoparasite have been observed. Comparing the 2D maps of the fungus grown in minimal medium with glycerol as carbon source (used as control condition) with those obtained in inducing conditions, a lot of novel proteins appeared. The higher number of novel and up-regulated spots was obtained in the presence of Rhizoctonia solani biomass. Other spots were specifically up-regulated by the interaction with different plant pathogens. Differentially expressed proteins were subjected to matrix assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) and N-terminal Edman sequencing. The in silico analyses of some of the novel and up-regulated spots showed interesting homology to hypothetical and putative proteins from other fungal species. These include novel enzymes, such as glycosylhydrolases and metalloprotease, proteins with conserved domains involved in pathogen-host interactions, such as Ras that regulates signal transduction pathways or LRRs that is involved in host recognition, etc. Work is in progress to demonstrate the role of some of these proteins in biocontrol and ability to induce systemic resistance. | Ambrosino P Scala V Marra R Vinale F Soriente I Ferraioli S Carbone V Ruocco M Woo S L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 1 |
| 14 | Use of Trichoderma spp. for biological control of the livestock feed contaminant fungus Fusarium proliferatum显示文摘Fusarium spp. are pathogens of many important agricultural crops, and are often strong mycotoxin producers. Fusarium proliferatum, in particular, causes disease in cereals and secretes the toxin Beauvaricin that contaminates livestock feed and cereals, producing a variety of toxicity symptoms ranging from poor weight gain to mortality. Beauvaricin is a cyclodepsipeptide and acts as a potent mycotoxin known to have insecticidal properties. This compound is highly toxic to human cell lines, where it induces apoptosis and specifically inhibits cholesterol acetyltransferase. Nothing is known about the role of this mycotoxin during the interaction of F. proliferatum with other microorganisms, including the fungal antagonists Trichoderma spp. In vitro tests have demonstrated that the antagonistic and mycoparasitic activity of Trichoderma is not inhibited by the presence of Beauvaricin at concentrations up to 10 mg/kg in the substrate. In vivo biocontrol assays on barley and wheat with Trichoderma against F. proliferatum isolates, producing and non-producing Beauvaricin, confirmed the ability of the antagonist to control this pathogen in all cases. Also Trichoderma culture filtrates obtained in conditions that promote Cell Wall Degrading Enzyme (CWDE) secretion, were able to inhibit. spore. germination. of. different. F.. proliferatum. isolates.. These. results. suggest. the. possibility. of. using. Trichoderma. and/or. its. metabolites. to. control. contaminants. of. livestock. feed. by. mycotoxin-producing. Fusarium.. | Ruocco M Pane F Ritieni A Lanzuise S Ambrosino P Marra R Woo S L Ciliento R Soriente I Ferraioli S Scala F Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 0 |
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