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| 1 | Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film显示文摘一个新奇电气化学的脱氧核糖核酸生物传感器基于氧化锌(ZnO ) ,为脱氧核糖核酸固定和提高的杂交察觉的 nanoparticles 和多围的碳 nanotubes (MWNT ) 被介绍。MWNTs/nano ZnO/chitosan 合成电影修改了玻璃化炭黑电极(MWNTs/ZnO/CHIT/GCE ) 被制作,脱氧核糖核酸探针在电极表面上被使不能调动。杂交事件被微分脉搏 voltammetry (DPV ) 作为指示物用亚甲蓝(MB ) 监视。传感器能有效地区别不同脱氧核糖核酸序列与有关在转基因的玉米轻拍基因,与 2.8 ×的检测极限 10 ? 目标顺序的 12 mol/L。 | Wei Zhang Tao Yang Da Ming Huang Kui Jiao | 2008 | Chinese Chemical Letters2008，19，5： | 8 |
| 2 | Molecular approaches identify known species, reveal cryptic species and verify host specificity of Chinese Philotrypesis (Hymenoptera: Pteromalidae)显示文摘 | MEI‐JIAO ZHOU JIN‐HUA XIAO SHENG‐NAN BIAN YAN‐WEI LI LI‐MING NIU HAO‐YUAN HU WEN‐SHAN WU ROBERT W. MURPHY DA‐WEI HUANG | 2012 | Molecular Ecology Resources2012，，4： | 1 |
| 3 | Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum显示文摘The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrir and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrir and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in them.Immunodotting results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic obserwtions further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes. | ZENG XIAN LU MING DA JIAO MIAO XING XIAO GUANG WANG SHUI HAO(Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China)(Department of Biology, Changchun Normal College,Changchun 130032, China) | 1999 | Cell Research1999，9，1： | 1 |
| 4 | Development and Assessment of Two Highly Pathogenic Avian Influenza(HPAI) H5N6 Candidate Vaccine Viruses for Pandemic Preparedness显示文摘Objective In China, 24 cases of human infection with highly pathogenic avian influenza(HPAI) H5 N6 virus have been confirmed since the first confirmed case in 2014. Therefore, we developed and assessed two H5 N6 candidate vaccine viruses(CVVs).Methods In accordance with the World Health Organization(WHO) recommendations, we constructed two reassortant viruses using reverse genetics(RG) technology to match the two different epidemic H5 N6 viruses. We performed complete genome sequencing to determine the genetic stability. We assessed the growth ability of the studied viruses in MDCK cells and conducted a hemagglutination inhibition assay to analyze their antigenicity. Pathogenicity attenuation was also evaluated in vitro and in vivo.Results The results showed that no mutations occurred in hemagglutinin or neuraminidase, and both CVVs retained their original antigenicity. The replication capacity of the two CVVs reached a level similar to that of A/Puerto Rico/8/34 in MDCK cells. The two CVVs showed low pathogenicity in vitro and in vivo, which are in line with the WHO requirements for CVVs.Conclusion We obtained two genetically stable CVVs of HPAI H5N6 with high growth characteristics,which may aid in our preparedness for a potential H5N6 pandemic. | LIU Li Qi LI Zi JIAO Ming LU Jian ZHOU Jian Fang LI Xi Yan LIU Jia GUO Jun Feng XIAO Ning ZHAO Xiang WANG Da Yan | 2020 | Biomedical and Environmental Sciences2020，33，9： | 0 |
| 5 | A New Ceramic Substrate Glaze with High Resistance to Molten Aluminum显示文摘Corrosion resistance of ceramic substrate glazes to molten aluminum was studied in this paper. The glazes can spreadslightly in aluminum alloy according to SEM examination of solidified interface between the glazes and aluminumalloy. The components of B2O3-P2O5 glazes were not detected with electron probe at the side of aluminum alloynear the interface, but the components of boron-free glaze were detected. It is shown that borophosphate glazes canresist the corrosion of molten aluminum. | Ming ZHOU, Ke LI, Da SHU, Jiao ZHANG, Baode SUN and Jun WANGState Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200030, China | 2003 | Journal of Materials Science & Technology2003，19，5： | 0 |
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