|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | Detecting chlorophyll Secchi disk depth and surfacee temperature in a sub-alpine lake using landsat irnagery显示文摘 | Claudia G Monica P Pietm A Paolo G Eugenio Z | 2001 | The Science of the Total Environment2001，268，： | 1 |
| 3 | Optimum voltage control for loss minimization in HVDC multi- terminal transmission systems for large offshore wind farms显示文摘 | Monica A P Agusti E A Oriol G B | 2012 | Electric Power Systems Research2012，，89： | 1 |
| 4 | Shape and size of micelles in the sodium dodecyl sul fate-formamide system显示文摘 | CEGLIE A COLAFEMMINA G MONICA M D | 1993 | Langmuir1993，9，： | 1 |
| 5 | 3, 4-Dicholoroaniline acute toxicity in male Fischer 344 rats显示文摘 | Monica A V Tagouri Y John G B | 1997 | Toxicology1997，124，： | 1 |
| 6 | Estimation of Mediterranean forest transpiration and photosynthesis through the use of an ecosystem simulation model driven by remotely sensed data显示文摘 | SILVIA A MARTA C MONICA G | 2004 | Global Ecology and Biogeography2004，13，4： | 1 |
| 7 | A horizontal pendulum tiltmeter with digital recording system显示文摘 | BELLA F MONICA G D ERIMINI A | 1989 | IL Nuovo Cimento1989，12，： | 1 |
| 8 | Reaction of dioxygen with n (Hdmpz = 3,5-dimethylpyrazole):crystal structure,reactivity,and catalytic properties of 显示文摘 | Ardizzoia G A Angaroni M A Monica G L | 1991 | Inorg Chem1991，30，： | 1 |
| 9 | Light guiding in oxidised porous silicon optical waveguides显示文摘 | Monica S La Frrand A | 1997 | Thin Solid Films1997，297，： | 1 |
| 10 | Shape and size of micelles in the sodium dodecyl sulfate-formamide system显示文摘 | CEGLIE A COLAFEMMINA G MONICA M D | 1993 | Langmuir1993，9，： | 1 |
| 11 | Structural revision of halipeptins, Synthesis of the thiazoline unit and isolation of halipeptinc显示文摘 | MONICA C D RANDAZZO A BIFULCO G | 2002 | Tetrahedron Letters2002，43，： | 1 |
| 12 | Transcriptional activation of the cyclin A gene by the architectural transcription factor HMGA2 显示文摘 | Michela A Tessari Monica G | 2003 | Mol Cell Biol2003，23，24： | 1 |
| 13 | Carbon monoxide in clouds at low metallic in the dwarf irregular galaxyWLM显示文摘 | Bruce G Elmegreen Monica Rubio Deidre A Hunter | 2013 | Nature2013，495，7442： | 1 |
| 14 | CaO supported on mesoporous silicas as basic catalysts for transesterification reactions 显示文摘 | Monica C G A Inmaculada J Jose S | 2008 | Applied Catalysis A: General2008，334，12： | 1 |
| 15 | Effectiveness of Helicobacter pylori eradication treatments in a primary care setting in Italy显示文摘 | Della Monica P Lavagna A Masoero G | 2002 | Aliment Pharmacol Ther2002，16，7： | 1 |
| 16 | Variation among populations of Clarkia unguiculata (Onagraceae) along altitudinal and latitudinal gradients显示文摘 | CHRISTINE S J MONICA A G | 1999 | American Journal of Botany1999，86，3： | 1 |
| 17 | Progress in the field integrated optoelectronics based on Porous Silicon显示文摘 | S La Monica G Maiello A Ferrari | 1996 | Thin Solid Films1996，267，： | 1 |
| 18 | Pregnancy outcomes after assisted reproductive technology 显示文摘 | Carlomagno G Di Blasi A Monica MD | 2006 | J Obstet Gynaecol Can2006，28，3： | 1 |
| 19 | Congenital seoliosis associ- ated with agenesis of the uterine cervix: Case report 显示文摘 | Carlomagno G Di Blasi A Monica MD | 2004 | Brnc Womens Health2004，4，1： | 1 |
| 20 | A horizontal pendulum tilmmter with digital recording system 显示文摘 | Bella F Delia G Monica Ermini A | 1989 | Ilnuovo Cimento1989，12，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员