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| 1 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 2 | On the generation of trajectories for multiple UAVs in environments with obstacles显示文摘 | Armando A N Douglas G M Mado F M | 2010 | J of Intelligent and Robotic Systems Archive2010，57，4： | 1 |
| 3 | A randomized trial of antibiotic prophylaxis for the prevention of surgical site infection after open mesh-plug hernia repair显示文摘 | Mazaki T Mado K Masuda H | 2014 | Am J Surg2014，207，4： | 1 |
| 4 | University of Puerto Rico has big plans for its Endowment Fund 显示文摘 | Mado Santana | 2009 | Caribbean Business Thursday August 132009，，： | 1 |
| 5 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair:a Bayesian and frequentist meta -analysis显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coll Surg2013，217，5： | 1 |
| 6 | Prophylactic pancreatic stent placement and post-ERCP pancreatitis: an updated meta-analysis显示文摘 | MAZAKI T MADO K MASUDA H | 2014 | J Interol2014，49，2： | 1 |
| 7 | A randomized trial of antibiotic prophylaxis for the prevention of surgical site infection after open mesh-plug hernia repair显示文摘 | Mazaki T Mado K Masuda H | 2014 | Am J Surg2014，207，4： | 1 |
| 8 | Antibiotic prophy- laxis for the prevention of surgical site infection after tension-free hernia repair: a bayesian and frequentist Meta-analysis 显示文摘 | MAZAKI T MADO K MASUDA H | 2013 | J Am Coil Surg2013，，13： | 1 |
| 9 | To vitro transformation of cell lines from human salivary gland tumors 显示文摘 | Quei mado L Lopes C Du F | 1999 | Int J Cancer1999，81，5： | 1 |
| 10 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair;a Bayesian and frequents meta-analysis显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coll Surg2013，217，5： | 1 |
| 11 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair: a Bayesian and frequentist meta-analysis显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coll Surg2013，217，5： | 1 |
| 12 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair:a Bayesian and frequentist meta-analysis显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coll Surg2013，217，5： | 1 |
| 13 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free he-nia repair:a Bayesian and frequentist raeta- analysis 显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coil Surg2013，217，5： | 1 |
| 14 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair:a Bayesian and frequentist meta-analysis 显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coil Surg2013，217，5： | 1 |
| 15 | A randomized trial of antibiotic prophylaxis for the prevention of surgical site infection after open mesh-plug hernia repair显示文摘 | Mazaki T Mado K Masuda H | 2014 | Am J Surg2014，207，4： | 1 |
| 16 | Language switching mechanisms in simultaneous interpreters: an ERP study显示文摘 | Alice Mado Proverbio Giuliana Leoni Alberto Zani | 2004 | Neuropsychologia2004，，12： | 1 |
| 17 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free hernia repair:a Bayesian and frequentist meta-analysis显示文摘 | Mazaki T Mado K Masuda H | 2013 | J Am Coll Surg2013，217，5： | 1 |
| 18 | Antibiotic prophylaxis for the prevention of surgical site infection after tension-free her- nia repair: a Bayesian and frequentist meta-analysis 显示文摘 | Mazaki T1 Mado K Masuda H | 2013 | J Am Coll Surg2013，217，5： | 1 |
| 19 | A randomized trial of antibi- otic prophylaxis for the prevention of surgical site infection after open mesh-plug hernia repair 显示文摘 | Mazaki T Mado K Masuda H | 2014 | Am J Surg2014，207，4： | 1 |
| 20 | Determination of Mycoplasma bovis susceptibilities against six antimicrobial agents using the E test method 显示文摘 | David Francoz Mado Fortin Gilles Fecteau | 2005 | Veterinary Microbiology2005，105，： | 1 |
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