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| 1 | Occult hepatitis B virus infection among Egyptian blood donors显示文摘AIM:To identify blood donors with occult hepatitis B virus(HBV) infection(OBI) to promote safe blood donation.METHODS:Descriptive cross sectional study was conducted on 3167 blood donors negative for hepatitis B surface antigen(HBsAg),hepatitis C antibody(HCV Ab) and human immunodeficiency virus Ab.They were subjected to the detection of alanine aminotransferase(ALT) and aspartate transaminase(AST) and screening for anti-HBV core antibodies(total) by two different techniques;[Monoliza antibodies to hepatitis B core(Anti-HBc) Plus-Bio-Rad] and(ARC-HBc total-ABBOT).Positive samples were subjected to quantitative detection of antibodies to hepatitis B surface(anti-HBs)(ETI-AB-AUK-3,Dia Sorin-Italy).Serum anti-HBs titers > 10 IU/L was considered positive.Quantitative HBV DNA by real time polymerase chain reaction(PCR)(QIAGEN-Germany) with 3.8 IU/mL detection limit was estimated for blood units with negative serum anti-HBs and also for 32 whose anti-HBs serum titers were > 1000 IU/L.Also,265 recipients were included,34 of whom were followed up for 3-6 mo.Recipients were investigated for ALT and AST,HBV serological markers:HBsAg(ETI-MAK-4,Dia Sorin-Italy),anti-HBc,quantitative detection of anti-HBs and HBV-DNA.RESULTS:525/3167(16.6%) of blood units were positive for total anti-HBc,64% of those were antiHBs positive.Confirmation by ARCHITECT anti-HBc assay were carried out for 498/525 anti-HBc positive samples,where 451(90.6%) confirmed positive.Reactivity for anti-HBc was considered confirmed only if two positive results were obtained for each sample,giving an overall prevalence of 451/3167(14.2%) for total anti-HBc.HBV DNA was quantified by real time PCR in 52/303(17.2%) of anti-HBc positive blood donors(viral load range:5 to 3.5 x 105 IU/mL) with a median of 200 IU/mL(mean:1.8 x 104 ± 5.1 x 104 IU/mL).AntiHBc was the only marker in 68.6% of donors.Univariate and multivariate logistic analysis for identifying risk factors associated with anti-HBc and HBV-DNA positivity among blood donors showed that age above thirty and marriage were the most significant risk factors for prediction of anti-HBc positivity with AOR 1.8(1.4-2.4) and 1.4(1.0-1.9) respectively.Other risk factors as gender,history of blood transfusion,diabetes mellitus,frequent injections,tattooing,previous surgery,hospitalization,Bilharziasis or positive family history of HBV or HCV infections were not found to be associated with positive anti-HBc antibodies.Among anti-HBc positive blood donors,age below thirty was the most significant risk factor for prediction of HBV-DNA positivity with AOR 3.8(1.8-7.9).According to HBV-DNA concentration,positive samples were divided in two groups;group one with HBV-DNA ≥ 200 IU/mL(n = 27) and group two with HBV-DNA < 200 IU/mL(n = 26).No significant difference was detected between both groups as regards mean age,gender,liver enzymes or HBV markers.Serological profiles of all followed up blood recipients showed that,all were negative for the studied HBV markers.Also,HBV DNA was not detected among studied recipients,none developed post-transfusion hepatitis(PTH) and the clinical outcome was good.CONCLUSION:OBI is prevalent among blood donors.Nucleic acid amplification/HBV anti core screening should be considered for high risk recipients to eliminate risk of unsafe blood donation. | Zeinab N Said Manal H El Sayed Iman I Salama Enas K Aboel-Magd Magda H Mahmoud Maged El Setouhy Faten Mouftah Manal B Azzab Heidi Goubran Amal Bassili Gamal E Esmat | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | CD5 expression promotes IL-10 production through activation of the MAPK/Erk pathway and upregulation of TRPC1 channels in B lymphocytes显示文摘CD5 is constitutively expressed on T cells and a subset of mature normal and leukemic B cells in patients with chronic lymphocytic leukemia(CLL).Important functional properties are associated with CD5 expression in B cells,including signal transducer and activator of transcription 3 activation,IL-10 production and the promotion of B-lymphocyte survival and transformation.However,the pathway(s)by which CD5 influences the biology of B cells and its dependence on B-cell receptor(BCR)co-signaling remain unknown.In this study,we show that CD5 expression activates a number of important signaling pathways,including Erk1/2,leading to IL-10 production through a novel pathway independent of BCR engagement.This pathway is dependent on extracellular calcium(Ca2+)entry facilitated by upregulation of the transient receptor potential channel 1(TRPC1)protein.We also show that Erk1/2 activation in a subgroup of CLL patients is associated with TRPC1 overexpression.In this subgroup of CLL patients,small inhibitory RNA(siRNA)for CD5 reduces TRPC1 expression.Furthermore,siRNAs for CD5 or for TRPC1 inhibit IL-10 production.These findings provide new insights into the role of CD5 in B-cell biology in health and disease and could pave the way for new treatment strategies for patients with B-CLL. | Soizic Garaud Taher E Taher Marjolaine Debant Miguel Burgos Sarra Melayah Christian Berthou Kaushal Parikh Jacques-Olivier Pers Damien Luque-Paz Gilles Chiocchia Maikel Peppelenbosch David A Isenberg Pierre Youinou Olivier Mignen Yves Renaudineau Rizgar A Mageed | 2018 | Cellular & Molecular Immunology2018，15，2： | 2 |
| 3 | A cold shock induced cyanobacterial RNA helicase显示文摘 | Chamot D Magee W C Yu E | 1999 | The Journal of Bacteriologyl1999，181，： | 1 |
| 4 | Quantifying nutrient requirements of fish 显示文摘 | Zeitoun I H Ullrey D E Magee W T | 1976 | Journal of Fisheries Research Board of Canada1976，33，： | 1 |
| 5 | Cytomagalovirus expresses the chemokine homologue vXCL1 capable of attracting XCR1 + CD4 - dendritic cells显示文摘 | Geyer H Hartung E Mages HW | 2014 | J Virol2014，88，1： | 1 |
| 6 | Use of the Ramberg- bicklund rearrangement for the synthesis of medium and large heterocyclic alkenes: stereoselective olefin formation显示文摘 | MAGEE D I BECK E J | 2000 | J Org Chem2000，65，24： | 1 |
| 7 | Hydralazine for treatment of severe hypertension in pregnancy : meta - analysis 显示文摘 | Magee LA Chain C Waterman E J | 2003 | BMJ2003，327，7421： | 1 |
| 8 | Antibiotic prescribing and antibiotic resistance in community practice:retrospective study,1996-1998 显示文摘 | Magee J T Pritchard E L Fitzgerald K A | 1999 | Br Med J1999，319，： | 1 |
| 9 | Hydralazine for treatment of severe hypertension in pregnancy: me- m-analysis显示文摘 | Magee LA Cham C Waterman E J | 2003 | Br Medl J2003，327，7421： | 1 |
| 10 | Toll‐like receptors 2 and 4 and the cryopyrin inflammasome in normal pregnancy and pre‐eclampsia显示文摘 | F Xie Y Hu SE Turvey LA Magee RM Brunham K‐C Choi M Krajden PCK Leung DM Money DM Patrick E Thomas P von Dadelszen | 2009 | BJOG: An International Journal of Obstetrics & Gy2009，，： | 1 |
| 11 | Quantifying nutrient requirements of fish显示文摘 | Zeitoun I H Ullrey D E Magee W T | 1976 | J Fish Res Board Can1976，33，： | 1 |
| 12 | Identification of two novel mutations in karation 13 as the cause of white sponge naevtts显示文摘 | Rugg E Magee G Wilson N | 1999 | Oral Dis1999，5，4： | 1 |
| 13 | The aetiology of vein graft stenoses显示文摘 | Davies AH Magee TR Sheffield E | 1994 | Eur J Vasc Surg1994，8，4： | 1 |
| 14 | Antibiotic prescribing and antibiotic resistance in community practice: retrospective study, 1996-8 显示文摘 | Magee J Pritchard E Fitzgerald A | 1999 | Br Med J1999，319，7219： | 1 |
| 15 | Response of wetland plant species to hydrologic conditions显示文摘 | MAGEE T K KENTULA M E | 2005 | Wetlands Ecology and Management2005，13，2： | 1 |
| 16 | Identification of two novel muta- tions in keratin 13 as the cause of white sponge naevus 显示文摘 | Rugg E Magee G Wilson N | 1999 | Oral Dis1999，5，4： | 1 |
| 17 | Short sleep duration is associated with risk of future diabetes but not cardiovascular disease : a prospective study and meta - analysis 显示文摘 | HOLLIDAY E G MAGEE C A KRITHARIDES L | 2013 | PLoS One2013，8，82： | 1 |
| 18 | Quantifying nutrient requirement of fish显示文摘 | ZEITOUN I H ULLREY D E MAGEE W T | 1976 | Journal of the Fisheries Research Board of Canada1976，33，： | 1 |
| 19 | Secure transmission with optimal power allocation in untrusted relay networks显示文摘 | Wang L F Maged E Jing H | 2014 | IEEE Wireless Communications Letters2014，3，3： | 1 |
| 20 | Cancer Stem Cells: Impact, Heterogeneity, and Uncertainty显示文摘 | Magee JA Piskounova E Morrison SJ | 2012 | Cancer Cell2012，21，3： | 1 |
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