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| 1 | 小反刍兽疫病毒(PPRV)N蛋白的表达与ELISA检测方法的建立显示文摘通过对下载的16株小反刍兽疫病毒(PPRV)N基因片段进行序列分析,合成了PPRV N基因。用NP1和NP2引物扩增。纯化的PPRV N基因在T4DNA连接酶的作用下,和PET32-a载体进行连接反应构建重组质粒pET-a-PPRV-N。经诱导表达和纯化后,得到了大量的PPRV N蛋白。用PPRV N蛋白制备免疫血清,经国际标准血清标化后作为参考阳性血清,同时以PPRV N蛋白为抗原建立间接ELISA(I-ELISA)方法。对山东省的467份羊血清检测,使用S/P(样品值/阳性值)平均值加3倍标准差的方法,计算出其临界点为0.28。应用间接ELISA和针对H蛋白的单抗为基础的C-ELISA检测来自于疫区的69份血清,结果两者的符合率为79.7%。 | 张喜悦 王志亮 刘春菊 李林 包静月 何昭阳 Mandy C Carrie B Anderson J | 2008 | 中国兽医学报2008，28，2： | 8 |
| 2 | Survival and recurrence after concomitant chemotherapy and radiotherapy for cancer of the uterine cervix: a systematic review and meta-analysis显示文摘 | John A Green John M Kirwan Jayne F Tierney Paul Symonds Lydia Fresco Mandy Collingwood Christopher J Williams | 2001 | The Lancet . 2001 (9284)2001，，： | 2 |
| 3 | Cytokines in the induction and resolution of experimental autoimmune encephalomyelitis 显示文摘 | MANDY J MCGEACHY M J ANDERTON SM | 2005 | Cytokine2005，32，： | 1 |
| 4 | Expression of CD44s and CD44v6 in transitional cell carcinomas of the urinary bladder:comparison with tumour grade,proliferative activity and p53 immunoreactivity of tumour cells显示文摘 | Kuncova J Urban M Mandys V | 2007 | APMIS2007，115，11： | 1 |
| 5 | Removal of Sulfur Compounds from Mineral Insulating Oils by Extractive Refining with N-methyl-2-pyrrolidone显示文摘 | Luki?J Nikoli?D Mandi?V | 2012 | Industrial&Engineering Chemistry Research2012，51，12： | 1 |
| 6 | Identification ofmicrosatellite markers for fragrance in rice by analysis of the rice genome sequence 显示文摘 | Giovanni M Cordeiro Mandy J Christopher Robert J Henry | 2002 | Molecular Breeding2002，9，4： | 1 |
| 7 | Pilon fractures 显示文摘 | Mandi DM Belin RP Banks J | 2012 | Clin Po- diatr Med Surg2012，29，2： | 1 |
| 8 | Pilon fractures 显示文摘 | Mandi DM Belin RP Banks J | 2012 | Clin Podiatr Med Surg2012，29，2： | 1 |
| 9 | The role of mathrix metalloproteinases in squamass cell carcinoma of the head and neck显示文摘 | Werner J A Rathcke I O Mandie R | 2002 | Clin Exp Metatasis2002，19，4： | 1 |
| 10 | T-cell subse Eounting and the fight against AIDS: reflection sorer a 20-year struggle' 显示文摘 | Mandy E Nieholson J Autran B | 2002 | Cytometry2002，50，： | 1 |
| 11 | Survival and recurrence after concomitant chemotherapy and radiotherapy for cancer of the uterine cervix: a systematic review and meta-analysis显示文摘 | John A Green John M Kirwan Jayne F Tierney Paul Symonds Lydia Fresco Mandy Collingwood Christopher J Williams | 2001 | The Lancet2001，，9284： | 1 |
| 12 | Pilon fractures显示文摘 | Mandi DM Belin RP Banks J | 2012 | Clin Podiatr Med Surg2012，29，2： | 1 |
| 13 | Use of streamflow data to estimate base flow/ground-water recharge for Wisconsin显示文摘 | Warren A G Mandy J R Ellen J C | 2007 | Journal of the American Water Resources Association2007，43，1： | 1 |
| 14 | Fractures of the forefoot 显示文摘 | Mandracchia V J Mandi D M Toney P A | 2006 | Clin Pediatr Med Surg2006，23，2： | 1 |
| 15 | A comparison of joint stability between anterior cruciate intact and deficient knees : a new canine model of anterior cruciate ligament disruption显示文摘 | Mandi J Lopez David Kunz Ray Vanderby Jr | 2003 | National Institutes of Health2003，21，2： | 1 |
| 16 | Diet in the Iron Age cemetery population at Wetwang Slack, East Yorkshire, UK: carbon and nitrogen stable isotope evidence 显示文摘 | Mandy J Richards M | 2006 | Journal of Archaeological Science2006，33，： | 1 |
| 17 | Thl7 cell differentiation: the long and winding road显示文摘 | Mandy J Daniel J | 2008 | Immunity2008，28，4： | 1 |
| 18 | Recent advances in milk clotting enzymes显示文摘 | MANDY J DORIS J HARALD R | 2010 | Int J Dairy Technol2010，64，1： | 1 |
| 19 | Molecular contribution to selection of intergeneric hybrids between sugarcane and the wild species Erianthus arundinaceus显示文摘 | George Piperidis Mandy J Christopher | 2000 | Genome2000，43，： | 1 |
| 20 | Gene expression in response to abscisic acid osmotic stress显示文摘 | Skrier K Mandy J | 1990 | Plant Cell1990，2，： | 1 |
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