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| 1 | Establishment of in Vitro Regeneration System of Bitter Melon (Momordica Charantia L. )显示文摘Explants of bitter melon can produce three types of callus: green, yellow green and fragile yellow callus. During the period of inducing callus, the explants are not sensitive to the kinds and proportions of phytohormone. The rate of callus inductivity is about 90.0%. However, in the process of callus differentiating adventitious bud, the kind, proportion and quantity of phytohormone and the type of callus made different result. The adventitious buds were induced successfully on MS medium+6-BA (N6-benzyladenine) 4.0 mg l-1+KT(kinetin) 2.0 mg l-1) by yellow green callus. The frequency was about 66.7 %. Yellow callus did not differentiated adventitious bud. The frequency of green callus differentiation was very low (<15.0 %) even under the most suitable conditions. The MS + ZT (zeatin) 5.0 mgl-1 +KT 0.5 mgl-1 medium was suitable for the proliferation of bud and three weeks later, the coefficient of proliferation was about 5-6. The 1/2 MS + ZT 0.02 mgl-1 or 1/2 MS media were suitable for in vitro rooting of shoot, the shoot on them could produce 6-7 new roots in three weeks. After plantation test, the survival rate of tube plantlets was about 70% and their characteristics were the same as those from seed by field test. | 杨满业 Zhao Maojun Zeng Yu Lan Liqong Chen Fang | 2004 | High Technology Letters2004，10，1： | 7 |
| 2 | Cloning and characterization of miRNAs from maize seedling roots under low phosphorus stress显示文摘 | Zhiming Zhang Haijian Lin Yaou Shen Jian Gao Kui Xiang Li Liu Haiping Ding Guangsheng Yuan Hai Lan Shufeng Zhou Maojun Zhao Shibin Gao Tingzhao Rong Guangtang Pan | 2012 | Molecular Biology Reports2012，，8： | 1 |
| 3 | Structural basis for a homodimeric ATPase subunit of an ECF transporter显示文摘The transition metal cobalt,an essential cofactor for many enzymes in prokaryotes,is taken up by several specifi c transport systems.The CbiMNQO protein complex be-longs to type-1 energy-coupling factor(ECF)transporters and is a widespread group of microbial cobalt transport-ers.CbiO is the ATPase subunit(A-component)of the cobalt transporting system in the gram-negative thermo-philic bacterium Thermoanaerobacter tengcongensis.Here we report the crystal structure of a nucleotide-free CbiO at a resolution of 2.3Å.CbiO contains an N-terminal canonical nucleotide-binding domain(NBD)and C-termi-nal helical domain.Structural and biochemical data show that CbiO forms a homodimer mediated by the NBD and the C-terminal domain.Interactions mainly via conserved hydrophobic amino acids between the two C-terminal do-mains result in formation of a four-helix bundle.Structural comparison with other ECF transporters suggests that non-conserved residues outside the T-component bind-ing groove in the A component likely act as a specifi city determinant for T components.Together,our data provide information on understanding of the structural organiza-tion and interaction of the CbiMNQO system. | Chengliang Chai You Yu Wei Zhuo Haifeng Zhao Xiaolu Li Na Wang Jijie Chai Maojun Yang | 2013 | Protein & Cell2013，4，10： | 1 |
| 4 | Application of bifunctional Saccharomyces cerevisiae to remove lead(II) and cadmium(II) in aqueous solution显示文摘 | Yunsong Zhang Weiguo Liu Li Zhang Meng wang Maojun Zhao | 2011 | Applied Surface Science2011，，23： | 1 |
| 5 | Cloning and Expression of the Serine Carboxypeptidase Gene in Zea mays and Its Antifungal Activity against Rhizoctonia solani显示文摘 | Li Liu Xiujing He Zhiming Zhang Maojun Zhao Jing Wang Haijian Lin Ya'ou Shenl Guangtang Pan | 2013 | Journal of Life Sciences2013，7，2： | 0 |
| 6 | A Large Acute Gastroenteritis Outbreak Associated with Both Campylobacter coli and Human Sapovirus—Beijing Municipality,China,2021显示文摘Summary What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of AGE worldwide.In a study conducted in Beijing,HuSaV detection ranked second after norovirus.What is added by this report?We present a discussion of the first large-scale outbreak of AGE caused by both Campylobacter coli(C.coli)and HuSaV.The outbreak involved a total of 996 patients and exhibited two distinct peaks over a period of 17 days.Through case-control studies,we identified exposure to raw water from a secondary water supply system as a significant risk factor.Among 83 patients,49 samples tested positive for C.coli,39 samples tested positive for HuSaV,and 27 samples tested positive for both pathogens using real-time polymerase chain reaction detection.Furthermore,whole-genome sequencing of 17 C.coli isolates obtained from 17 patients revealed that all isolates belonged to a highly clonal strain of C.coli.What are the implications for public health practice?Outbreaks of AGE resulting from multiple pathogen infections warrant increased attention.This report emphasizes the significance of ensuring the safety of drinking water,particularly in secondary supply systems. | Lin Zou Ying Li Guilan Zhou Zhenzhou Huang Changyan Ju Chunyan Zhao Xiang Gao Bojun Zhen Ping Zhang Xiaochen Guo Jing Zhang Yang Zhang Bo Liu Shaolei Zhou Aixia Yan Ying Kang Yanchun Wang Hongmei Ma Xiaohui Li Maojun Zhang | 2023 | China CDC weekly2023，5，52： | 0 |
| 7 | Identification and functional analysis of miRNAs in developing kernels of a viviparous mutant in maize显示文摘Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In this study, we constructed a small RNA library to comprehensively represent the full complement of individual small RNAs and to characterize miRNA expression profiles in pooled ears of maize(Zea mays L.) at 10, 15,20, 22, 25 and 30 days after pollination(DAP). At least 21 miRNAs were differentially expressed. The differential expression of three of these miRNAs, i.e., miR528a, miR167a and miR160b, at each stage was verified by qRT-PCR. The results indicated that these miRNAs might be involved in kernel development. In addition, the predicted functions of target genes indicated that most of the target genes are involved in signal transduction and cell communication pathways, particularly the auxin signaling pathway. The expression of candidate germination-associated miRNAs was analyzed by hybridization to a maize genome microarray, and revealed differential expression of genes involved in plant hormone signaling pathways. This finding suggests that phytohormones play a critical role in the development of maize kernels. We found that in combination with other miRNAs, miR528a regulated a putative laccase, a Ring-H2 zinc finger protein and a MADS box-like protein, whereas miR167a and miR160b regulated multiple target genes,including ARF(auxin response factor), a member of the B3 transcription factor family. All three miRNAs are important for ear germination, development and physiology. The small RNA transcriptomes and mRNA obtained in this study will help us gain a betterunderstanding of the expression and function of small RNAs in the development of maize kernel. | Haiping Ding Jian Gao Mao Luo Hua Peng Haijian Lin Guangsheng Yuan Yaou Shen Maojun Zhao Guangtang Pan Zhiming Zhang | 2013 | The Crop Journal2013，1，2： | 0 |
| 8 | Vaccination inhibits TLR2 transcription via suppression of GR nuclear translocation and binding to TLR2 promoter in porcine lung infected with Mycoplasma hyopneumoniae显示文摘Toll-like receptors(TLRs)and glucocorticoid receptor(GR)act as effectors of innate immune system and neuroendocrine system,respectively.The crosstalk between these two receptors is important to maintain homeostasis during immune response.It is well known that vaccination can boost adaptive immune responses to specific pathogens.However,it remains elusive whether and | Zhiyuan Sun Maojun Liu Xian Li Huafeng Zou Guoqing Shao Ruqian Zhao | 2012 | 畜牧与兽医2012，44，S1： | 0 |
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