|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Excitotoxic damage,disrupted energy metabolism,and oxidative stress in the rat brain:antioxidant and neuroprotective effects of L-carnitine显示文摘 | DANIELA S A VERONICA P D C MARA N H M | 2008 | Jonrnal of Neurochemistry2008，105，： | 1 |
| 3 | Specific polymerase chain reaction for differential diagnosis of Dirofilaria immitis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2)显示文摘 | Mar P H Yang I C Chang G N | 2002 | Veterinary Parasitology2002，106，3： | 1 |
| 4 | Identification of a retinoic acid responsive element in the retinoic acid receptor beta gene 显示文摘 | DE THE H DEL MAR VR M TIOLLAIS P | 1990 | Nature1990，343，： | 1 |
| 5 | Evaluation of an indirect ELISA for detection of antibodies in bulk milk against bluetongue virus infections in The Netherlands显示文摘 | Mars M H van Maanen C Ve]lema P | | 0，，： | 1 |
| 6 | Identification of a retinoic acid responsive element in the retinoic acid receptor & beta gene显示文摘 | De The H Del Mar Vivanco-Ruiz M Tiollais P | 1990 | Nature1990，343，6254： | 1 |
| 7 | Monitoring of BVDV in a vaccinated herd by testing milk for antibodies to NS3 protein显示文摘 | Kuijk H Franken P Mars M H | 2008 | Vet Rec2008，163，16： | 1 |
| 8 | Photocurrent spectroscopy of optical absorption enhancement in siliconphotodiodes via scattering from surface plasmon polaritons in gold nanoparticles 显示文摘 | LIM S H MAR W MATHEU P | 2007 | J Appl Phys2007，101，10： | 1 |
| 9 | Association of the apolipoprotein A1/C3/A4/A5 gene cluster with triglyceride levels and LDL particle size in familial combined hyperlipidemia显示文摘 | Mar R Pajukanta P Allayee H | 2004 | Circ Res2004，94，7： | 1 |
| 10 | Stable lead isotope tracers of air mass trajectories in the Mediterranean region显示文摘 | Marring H Settle D M Menard P B | 1987 | Nature1987，300，： | 1 |
| 11 | Trend analysis of reference evapotranspiration in the western half of Iran 显示文摘 | I Tabari H Mar'off S Hosseinzadeh Talaee P | 2011 | Agric For Meteoro12011，151，： | 1 |
| 12 | Photocurrent spectros- copy of optical absorption enhancement in silicon photodiodes via scattering form surface plasmon polaritons in gold nano- partices 显示文摘 | LIM S H MAR W MATHEU P | 2007 | J Appl Phys2007，101，10： | 1 |
| 13 | Metabolic engineering of mannitol production in Lactococcus lactis: influence of overexpression of mannitol 1-phosphate dehydrogenase in different genetic backgrounds显示文摘 | WISSELINK H W MARS A E VAN DER MEER P | 2004 | Appl Environ Microbiol2004，70，： | 1 |
| 14 | Human brucellosis显示文摘 | María Pía Franco Maximilian Mulder Robert H Gilman Henk L Smits | 2007 | The Lancet Infectious Diseases2007，，12： | 1 |
| 15 | Photocurrent spectroscopy ofoptical absorption enhancement via scattering from surfaceplasmon polaritons in gold nanoparticles 显示文摘 | Lim S H Mar W Matheu P | 2007 | Journal of AppliedPhysics2007，101，10： | 1 |
| 16 | Photocatalytic oxidation of the antibiotic tetracycline on TiO 2 and ZnO suspensions显示文摘 | Rodrigo A. Palominos María A. Mondaca Ana Giraldo Gustavo Pe?uela Montserrat Pérez-Moya Héctor D. Mansilla | 2009 | Catalysis Today2009，，1： | 1 |
| 17 | Specific polymerase chain reaction for differential diagnosis of Diro filaria iumol /Litis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2) 显示文摘 | Mar P H Yang I C Chang G N | 2002 | Veterinary Parasitology2002，106，3： | 1 |
| 18 | When financial incen- tives do more good than harm: a checklist显示文摘 | Glasziou P P Buchan H Del Mar C | 2012 | British Medical Jour- nal2012，345，： | 1 |
| 19 | Photocurrent spectroscopy of optical absorption enhancement in silicon photodiodes via scattering from surface plasmon polaritons in gold nanoparticles显示文摘 | Lim S H Mar W Matheu P | | 0，，： | 1 |
| 20 | Immunochemical detection of adducts of sulfur mustard to DNA of calf thymus and human white blood cells显示文摘 | van der SCHANS G P SCHEFFER A G MARS GROENENDIJK R H | 1994 | Chem Res Toxicol1994，7，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员