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| 1 | Design of multiplex calibrant plasmids,their use in GMO detection and the limit of their applicability for quantitative purposes owing to competition effects显示文摘 | Debode F Mariena Janssen E | 2010 | Analitical and Bio-analytical Chemistry2010，396，： | 1 |
| 2 | 马铃薯X病毒外壳蛋白基因片段的瞬时表达诱导对PVX的高抗性(英文)显示文摘为获得抗马铃薯X病毒(PVX)的植株,从疑似感染马铃薯X病毒的马铃薯叶片中提取出PVXcp cDNA,将其保守片段插入到RNAi质粒pHellsgate12中以构建编码发夹结构RNA的载体,通过Agroinfiltration的方法在本氏烟植株中瞬时表达。结果表明:病毒侵染10d后,15株引入该载体的本氏烟植株对PVX均具有抗性,此载体可用于产生对马铃薯X病毒具有高抗性的转基因植物。 | 黄永会 陶刚 朱英 Mariena Ketudat-Cairns 邱礽 刘永翔 刘作易 | 2011 | 贵州农业科学2011，39，2： | 1 |
| 3 | Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes显示文摘 | Theppanya Charoenrat Mariena Ketudat-Cairns Helle Stendahl-Andersen Mehmedalija Jahic Sven-Olof Enfors | 2005 | Bioprocess and Biosystems Engineering2005，，6： | 1 |
| 4 | Oxygenlimited fed-batch process:an alternative control for Pichia pastoris recombinant protein process显示文摘 | THEPPANYA C MARIENA K C HELLE S A | 2005 | Bioprocess and Biosystems Engineering2005，27，： | 1 |
| 5 | A model to develop biological probes from microflora to assure traceability of tilapia 显示文摘 | Darawan R Saowant T Mariena K C | 2011 | Food Control2011，22，11： | 1 |
| 6 | Recovery of recombinant β-glucosidase by expanded bed adsorption from Pichia pastoris high-cell-density culture broth显示文摘 | Theppanya Charoenrat Mariena Ketudat-Cairns Mehmedalija Jahic Sven-Olof Enfors Andres Veide | 2005 | Journal of Biotechnology2005，，1： | 1 |
| 7 | Optimization of Quantitative Real-time PCR System on Amplification of Beta-glucosidase Gene Os1bglu4显示文摘[Objective] This study aimed to establish a quantitative real-time PCR(qRT-PCR) system for detecting the expression of rice beta-glucosidase gene Os1bglu4. [Method] The PCR was conducted with SYBR Green I method, using the primers of reference gene actin or ubiquitin. [Result] Actin was more suitable to be the reference gene than ubiquitin. More accurate results were obtained when the 100 ng cDNA template was added at a large volume and a lower concentration.The primer concentration in the range from 0.2 to 0.8 μmol/L we set had no significant influence on the results, so, 0.4 μmol/L was selected as the optimal primer concentration in this study. The amplification efficiency was greatly reduced when the annealing temperature was set at 64 ℃, therefore, annealing temperature was set at 60 ℃. Compared with the reaction system of 25 μl, the fluorescence intensity was significantly lower but the CT value did not change greatly in 10 μl system.So, the 10 μl reaction system was selected, which significantly reduces the research costs for the detection of a large amount of samples in future study. | Rouyi CHEN Jiang CHENG Changxiang ZHENG Minna PAN Mariena KETUDAT-CAIRNS | 2014 | Agricultural Science & Technology2014，15，7： | 0 |
| 8 | Evaluation on Inducible Effect of pOp6 Promoter in Transgenic Rice显示文摘In order to analyze the Os1bglu4 phenotype,the inducible promoter of the transgenic rice which knock-down the Os1bglu4 expression was assessed.The result showed that 30μM dexamethasone(DEX)had the stronger induction effect than10μM DEX byβ-Glucuronidase(GUS)staining.qRT-PCR further verified the Os1bglu4 gene deletion.The effect of DEX and its solvent absolute ethanol on seed development was measured,and no significant effect was observed.The conclusion is that final concentration of DEX at 30μM is suitable for pOp6 promoter induction. | Rouyi CHEN Changxiang ZHENG Jiang CHENG Minna PAN Mariena KETUDAT-CAIRNS | 2014 | Agricultural Science & Technology2014，15，5： | 0 |
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