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您的检索式：作者名="Markus R.Wenk"
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| 1 | Comprehensive Analysis of Lipid Composition in Crude Palm Oil Using Multiple Lipidomic Approaches显示文摘Palm oil is currently the leading edible oil consumed worldwide. Triacylglycerol(TAG) and diacylglycerol(DAG) are the dominant lipid classes in palm oil. Other lipid classes present in crude palm oil, such as phospholipids and galactolipids, are very low in abundance.These low-abundance lipids constitute key intermediates in lipid biosynthesis. In this study, we applied multiple lipidomic approaches,including high-sensitivity and high-specificity multiple reaction monitoring, to comprehensively quantify individual lipid species in crude palm oil. We also established a new liquid chromatography-coupled mass spectrometry method that allows direct quantification of low-abundance galactolipids in palm oil without the need for sample pretreatment. As crude palm oil contains large amounts of neutral lipids, our direct-detection method circumvents many of the challenges encountered with conventional lipid quantification methods. This approach allows direct measurement of lipids with no hassle during sample preparation and is more accurate and precise compared with other methods. | Wei Fun Cheong Markus R.Wenk Guanghou Shui | 2014 | Journal of Genetics and Genomics2014，41，5： | 1 |
| 2 | AB013.Tear lipidome and its implications显示文摘The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health.The composition of the tear film amphiphilic lipid sublayer,in particular,has largely remained a matter of contention.The limiting concentrations of lipid amphiphiles in tears have also posed considerable challenges to their detection and accurate quantitation.Using systematic and sensitive lipidomic approaches,we reported the most comprehensive human tear lipidome to date;and conferred novel insights to the compositional details of the existent tear film model,in particular the disputable amphiphilic lipid sublayer constituents,by demonstrating the presence of cholesteryl sulfate,O-acyl-ω-hydroxy fatty acids,and various sphingolipids and phospholipids in tears.Lipidomic analysis of human tear fluid from patients with various subtypes of dry eye syndrome(DES)revealed structure-specific lipid alterations in DES,which could potentially serve as unifying indicators of disease symptoms and signs.The meibomian glands constitute the predominant source of lipid supply to the human tear fluid.Meibomian gland dysfunction(MGD)is a leading cause of evaporative dry eye and ocular discomfort,characterized by an unstable tear film principally attributed to afflicted delivery of lipids to the ocular surface.We investigated the longitudinal tear lipid alterations associated with disease alleviation and symptom improvement in a cohort of MGD patients undergoing eyelid-warming treatment for 12 weeks.Our preliminary data indicated that excess ocular surface phospholipase activity detrimental to tear film stability could be alleviated by eyelid warming alone without application of steroids and identify tear OAHFAs as suitable markers to monitor treatment response in MGD. | Sin Man Lam Louis Tong Markus R.Wenk Guanghou Shui | 2017 | Annals of Eye Science2017，，1： | 0 |
| 3 | MFSD7c functions as a transporter of choline at the blood–brain barrier显示文摘Mutations in the orphan transporter MFSD7c(also known as Flvcr2),are linked to Fowler syndrome.Here,we used Mfsd7c knockout(Mfsd7c–/–)mice and cell-based assays to reveal that MFSD7c is a choline transporter at the blood–brain barrier(BBB).We performed comprehensive metabolomics analysis and detected differential changes of metabolites in the brains and livers of Mfsd7c–/–embryos.Particularly,we found that choline-related metabolites were altered in the brains but not in the livers of Mfsd7c–/–embryos.Thus,we hypothesized that MFSD7c regulates the level of choline in the brain.Indeed,expression of human MFSD7c in cells significantly increased choline uptake.Interestingly,we showed that choline uptake by MFSD7c is greatly increased by choline-metabolizing enzymes,leading us to demonstrate that MFSD7c is a facilitative transporter of choline.Furthermore,single-cell patch clamp analysis showed that the import of choline by MFSD7c is electrogenic.Choline transport function of MFSD7c was shown to be conserved in vertebrates,but not in yeasts.We demonstrated that human MFSD7c is a functional ortholog of HNM1,the yeast choline importer.We also showed that several missense mutations identified in patients exhibiting Fowler syndrome had abolished or reduced choline transport activity.Mice lacking Mfsd7c in endothelial cells of the central nervous system suppressed the import of exogenous choline from blood but unexpectedly had increased choline levels in the brain.Stable-isotope tracing study revealed that MFSD7c was required for exporting choline derived from lysophosphatidylcholine in the brain.Collectively,our work identifies MFSD7c as a choline exporter at the BBB and provides a foundation for future work to reveal the disease mechanisms of Fowler syndrome. | Xuan Thi Anh Nguyen Thanh Nha Uyen Le Toan Q.Nguyen Hoa Thi Thuy Ha Anna Artati Nancy C.P.Leong Dat T.Nguyen Pei Yen Lim Adelia Vicanatalita Susanto Qianhui Huang Ling Fam Lo Ngah Leong Isabelle Bonne Angela Lee Jorge L.Granadillo Catherine Gooch Dejie Yu Hua Huang Tuck Wah Soong Matthew Wook Chang Markus R.Wenk Jerzy Adamski Amaury Cazenave-Gassiot Long N.Nguyen | 2024 | Cell Research2024，34，3： | 0 |
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