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| 1 | Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells显示文摘AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 F-proline.RESULTS Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated PSCs.CONCLUSION The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in vitro, but cannot reverse the phenotype once the cells are fully activated. | Peter Wallbaum Sarah Rohde Luise Ehlers Falko Lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster | 2018 | World Journal of Gastroenterology2018，24，2： | 3 |
| 2 | MapMan4: A Refined Protein Classification and Annotation Framework Applicable to Multi-Omics Data Analysis显示文摘Genome sequences from over 200 plant species have already been published, with this number expected to increase rapidly due to advances in sequencing technologies. Once a new genome has been assembled and the genes identified, the functional annotation of their putative translational products, proteins, using ontologies is of key importance as it places the sequencing data in a biological context. Furthermore, to keep pace with rapid production of genome sequences, this functional annotation process must be fully automated. Here we present a redesigned and significantly enhanced MapMan4 framework, together with a revised version of the associated online Mercator annotation tool. Compared with the original MapMan, the new ontology has been expanded almost threefold and enforces stricter assignment rules. This framework was then incorporated into Mercator4, which has been upgraded to reflect current knowledge across the land plant group, providing protein annotations for all embryophytes with a comparably high quality. The annotation process has been optimized to allow a plant genome to be annotated in a matter of minutes. The output results continue to be compatible with the established MapMan desktop application. | Rainer Schwacke Gabriel Y.Ponce-Soto Kirsten Krause Anthony M.Bolger Borjana Arsova Asis Hallab Kristina Gruden Mark Stitt Marie E.Bolger Bjorn Usadel | 2019 | Molecular Plant2019，12，6： | 2 |
| 3 | Bisoprolol dose-response relationship in study(CIBIS Ⅱ)显示文摘 | SIMON T MARY KRAUSE M FUNCK-BRENTANO C | 2003 | Eur Heart J2003，24，： | 1 |
| 4 | Genetic Determinants of response to Clopidogrel and cardiovascular events 显示文摘 | Simon T Verstuyft C Mary - Krause M | 2009 | N Engl J Med2009，360，4： | 1 |
| 5 | Bisoprololdosc-response relationship in study (CBISⅡ)显示文摘 | Smont Mary Krause M | 2003 | Eur Heart J2003，24，： | 1 |
| 6 | Genetic determi nants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyft C Mary Krause M | 2009 | N Engl J Med2009，360，4： | 1 |
| 7 | Understanding and pre?venting subacute rumina] acidosis in dairy herds: A review显示文摘 | Krause K Marie Oetzel Garrett R | 2006 | Animal Feed Science and Technology2006，126，3： | 1 |
| 8 | Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population显示文摘 | Susanne Krause Gerald Reese Stefanie Randow Danila Zennaro Donato Quaratino Paola Palazzo Maria Antonietta Ciardiello Arnd Petersen Wolf-Meinhard Becker Adriano Mari | 2009 | The Journal of Allergy and Clinical Immunology2009，，4： | 1 |
| 9 | Genetic determi- nants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyh C Mary Krause M eta | 2009 | N Engl J Med2009，360，36： | 1 |
| 10 | Geneti determinants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyft C Mary - Krause M | 2009 | N Engl J Med2009，360，4： | 1 |
| 11 | Bisoprolol dose-response relationship in patients with congestive heart failure:a subgroup analysis in the cardiac insufficiency bisoprolol study(CIBIS Ⅱ)显示文摘 | SIMON T MARY KRAUSE M FUNCK BRENTANO C | 2003 | Eur Heart2003，24，6： | 1 |
| 12 | Genetic determinants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyft C Mary - Krause M | 2009 | N Engl J Med2009，360，4： | 1 |
| 13 | Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population显示文摘 | Susanne Krause Gerald Reese Stefanie Randow Danila Zennaro Donato Quaratino Paola Palazzo Maria Antonietta Ciardiello Arnd Petersen Wolf-Meinhard Becker Adriano Mari | 2009 | The Journal of Allergy and Clinical Immunology2009，，4： | 1 |
| 14 | Genetic de terminants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyft C Mary Krause M | 2009 | N Engl J Med2009，360，4： | 1 |
| 15 | Genetic determi- nants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyft C Mary - Krause M | 2009 | The New England journal of medicine2009，360，4： | 1 |
| 16 | Genetic determinants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Verstuyft C Mary Krause M | 2009 | N Engl J Med2009，360，4： | 1 |
| 17 | Genetic determinants of response to clopidogrel and cardiovascular events 显示文摘 | Simon T Verstuyft C Mary - Krause M | 2009 | N Engl J Med2009，360，： | 1 |
| 18 | Genetic determi- nants of response to clopidogrel and cardiovascular events显示文摘 | Simon T Vemmyft C Mary ~ Krause M | 2009 | N Engl J Med2009，3604，： | 1 |
| 19 | Female gender is an independent predictor of survival in patient with chronic heart failure:results from the cardiac insufficiency bisoprolol study(CIBIS Ⅱ ) 显示文摘 | Mary - Krause M Funck - Brentano C | 1999 | Circulation1999，100，1： | 1 |
| 20 | Genetic determinants of response to clopidogrel and cardiovascular events 显示文摘 | SimonI Verstuyfi C Mary - Krause M | 2009 | N Engl J Med2009，360，5： | 1 |
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