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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | On the mechanism of re- sistance to diclofop-methyl 显示文摘 | HOLTUM JAM MATTHEWS JM H USLER RE et aL Cross-resistance to herbicides in annual ryegrass (LoLium rigidum) Ⅲ | 1991 | Plant Physiol1991，97，： | 1 |
| 3 | Sleep Duration and Insulin Resistance in Healthy Black and White Adolescents 显示文摘 | Matthews KA Dahl RE Owens JF | 2012 | Sleep2012，35，10： | 1 |
| 4 | Long-distance pollen flow assessment through evaluation of pollinator forag- ing range suggests transgene escape distances 显示文摘 | RE'MY S P Alexis P Matthew B H | 2008 | Proceedings of the National Academy of Sciences of the United States of America2008，105，13： | 1 |
| 5 | Gamma knife surgery in the management of brain metastases from lung carcinoma:a retrospective analysis of survival,local tumor control,and freedom from new brain metastasis显示文摘 | Jawahar A Matthew RE Minagar A | 2004 | J Neurosurg2004，100，5： | 1 |
| 6 | Gamma knife surgery in the management of brain metastases from lung car- cinoma: a retrospective analysis of survival, local tumor control, and freedom from new brain metastasis 显示文摘 | Jawahar A Matthew RE Minagar A | 2004 | J Neurosurg2004，100，5： | 1 |
| 7 | Frontotemporal lobar degeneration epidemiology,pathology,diagnosis and management显示文摘 | Seltman RE Matthews BR | 2012 | CNS Drugs2012，26，： | 1 |
| 8 | Gamma knife surgery in the management of brain metastases from lung carcinoma: a retrospective analysis of survival, local tumor control, and freesom from new brain metastasis 显示文摘 | Jawahar A Matthew RE Minagar A | 2004 | J Neurosurg2004，，： | 1 |
| 9 | Frontotemporal lobar degeneration:epidemiology,pathology,diagnosis and management显示文摘 | Seltman RE Matthews BR | 2012 | CNS Drugs2012，26，： | 1 |
| 10 | Plasma cell dyscrasia causing light chain tubulopathy without fanconi syndrome显示文摘 | Matthew RE Cherise C Alvaro MA | 2010 | American Journal of Kidney Diseases2010，55，6： | 1 |
| 11 | Frontotemporal lobar degeneration: epidemiology, pathology, diagnosis and management 显示文摘 | Sehman RE Matthews BR | 2012 | CNS Drugs2012，26，： | 1 |
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