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您的检索式：作者名="Matthias Fuest"
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| 1 | Tonom-etry in Corneal Edema after Cataract Surgery:Rebound ver- sus Goldmann Applanat Tonometry 显示文摘 | Matthias Fuest Nikolaos Mamas Peter Walter | 2014 | Current Eye Re- search2014，39，9： | 1 |
| 2 | In vitro eye embryogenesis from induced pluripotent stem cells for ocular surface disease treatment显示文摘In their letter to Nature,Hayashi et al.describe a new method for the generation of ocular surface epitheliumlike cells by incubating human induced pluripotent stem cells(iPS)into four defined concentric zones mimicking the developing eye,which the authors named as self-formed ectodermal autonomous multi-zone(SEAM)(1). | Matthias Fuest Jodhbir S.Mehta | 2016 | Eye Science2016，31，4： | 0 |
| 3 | A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal stromal keratocytes for stromal cell therapy显示文摘Background:Human corneal stromal keratocytes propagated in culture media supplemented with human amnion extract(AME)can correct early corneal haze in an animal model.Clinical application of cultivated keratocytes is limited by infectious disease screening before amnion products can be used in humans.It remains unclear if AME from cryopreserved versus fresh human amnion can support human keratocyte propagation,and which components of the extract promote keratocyte growth.Methods:Three placentas were collected for the preparation of fresh and cryopreserved amnion tissues followed by homogenization and protein extraction.AME protein profiles were studied using isobaric tagging for relative and absolute quantitation(iTRAQ)proteomics.Enriched gene ontology(GO)terms and functional classes were identified.Primary human keratocytes from 4 donor corneas were cultured in media supplemented with fresh AME(F-AME)or cryopreserved AME(C-AME).Cell viability,proliferation and keratocyte marker expression were examined by confocal immunofluorescence and flow cytometry.Results:AME proteomics revealed 1385 proteins with similar expression levels(between 0.5-and 2-fold)between Fand C-AME,while 286 proteins were reduced(less than 0.5-fold)in C-AME.Enriched GO term and biological pathway analysis showed that those proteins with comparable expression between F-AME and C-AME were involved in cell metabolism,epithelial-mesenchymal transition,focal adhesion,cell-extracellular matrix interaction,cell stress regulation and complement cascades.Human corneal stromal keratocytes cultured with F-AME or C-AME showed similar morphology and viability,while cell proliferation was mildly suppressed with C-AME(P>0.05).Expression of aldehyde dehydrogenase 3A1(ALDH3A1)and CD34 was similar in both cultures.Conclusion:AME from cryopreserved amnion had limited influence on keratocyte culture.It is feasible to use protein extract from cryopreserved amnion to propagate human keratocytes for potential translational applications. | Beau J.Fenner Nur Zahirah B.M.Yusoff Matthias Fuest Lei Zhou Francisco Bandeira Howard Y.Cajucom-Uy H.K.Tan Jodhbir S.Mehta Gary H.F.Yam | 2019 | Eye and Vision2019，6，1： | 0 |
| 4 | Acupuncture for the management of dry eye disease显示文摘The effectiveness of using acupuncture for dry eye disease(DED)is controversial.Thus,this systematic review investigated the effectiveness and feasibility of using acupuncture for DED in accordance with the 2020 PRISMA statement.The outcomes of interests were(1)to evaluate the efficacy of acupuncture in improving the ocular surface disease index(OSDI),Schirmer I test score,and tear breakup time from baseline to the last follow-up;(2)to determine possible complications of using acupuncture;and(3)to investigate the superiority of acupuncture over other commonly used treatments for DED.Data from 394 patients were collected.Results showed that acupuncture significantly prolonged the tear breakup time(P<0.0001),significantly increased the Schirmer I test score(P<0.0001),and significantly reduced the OSDI(P<0.0001)from baseline to the last follow-up.Compared with the control group,the acupuncture group had significantly greater Schirmer I test score(P<0.0001),significantly longer tear breakup time(P=0.0004),and significantly lower OSDI(P=0.002).These results suggest that acupuncture is effective and feasible in improving symptoms and signs of DED.No severe adverse effects of acupuncture were observed. | Julia Prinz Nicola Maffulli Matthias Fuest Peter Walter Frank Hildebrand Filippo Migliorini | 2022 | Frontiers of Medicine2022，16，6： | 0 |
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