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| 1 | Expression of the oxygen-sensitive transcription factor subunit HIF-1α in patients suffering from secondary Raynaud syndrome显示文摘Anti-ischemic therapy remains a challenge due to the complexity of hypoxia response pathways. Hypoxia-inducible factor (HIF)-1 is a heterodimer tran scription factor con sisti ng of 2 sub units, HIF-1α and HIF-1β. Hypoxia-depe ndent activatio n of HIF-1α regulates cellular 02 homeostasis. Raynaud syndrome (RS), as a comorbidity of the autoimmune disease systemic sclerosis (SS), is characterized by vasospasms that limit blood flow to the limbs, resulting in hypoxia. A single-center randomized study was con ducted to compare prostagla ndin E1 (PgEI) therapy with a treatme nt combi ning PgE1 and an en dotheli n-1 blocker, bosentan. A total of 30 patients suffering from SS with RS were enrolled. We examined the regulation of HIF-1α, its target heme oxygenase-1 (HMOX-1), and the serum levels of the HIF-1α protein in a subset of patients as well as in ten healthy individuals. The expression of HIF-1α and HMOX-1 in monocytes was measured using absolute plasmid-based quantitative real-time PCR, whereas serum HIF-1α levels were measured with ELISA. Samples were taken at the time of randomization and after 24 weeks. We found that HIF-1α and HMOX-1 mRNA expression in monocytes and serum HIF-1α protein levels were significantly higher in the SS/RS patients compared to the healthy control group. Single-drug therapy significantly increased HIF-1α and HMOX-1 mRNA expression in monocytes and serum HIF-1α protein levels in the SS/RS patients compared to those at the time of randomization, whereas combining PgE1 with an en dotheli n-1 blocker preve nted the further in creases in HIF-1α and HMOX-1 expressi on. We propose HIF-1α and HMOX-1 as novel markers for anti-ischemic therapy in RS. | Lukas Andreas Heger Mark Kerber Marcus Hortmann Samuel Robinson Maximilian Mauler Daniela Stallmann Daniel Duerschmied Christoph Bode Christoph Hehrlein Ingo Ahrens | 2019 | Acta Pharmacologica Sinica2019，40，4： | 5 |
| 2 | Chip-based digital PCR as a novel detection method for quantifying microRNAs in acute myocardial infarction patients显示文摘miRNAs 为尖锐心肌的梗塞(AMI ) 作为潜在的 biomarkers 显示出诺言。然而,当前的使用的量的即时 PCR (qRT-PCR ) 完全为 nucleic 酸的相对表示允许,它产生日常可变性,它限制了把 miRNAs 用作 biomarkers 的有效性。在这研究,我们探索了技术质量和一种新技术的诊断潜力,基于薄片的数字 PCR,在确定在有 AMI 和 ischaemia-reperfusion 损害(I/R ) 的病人的 miRNAs。在合成 C.elegans-miR-39 的一个冲淡系列,基于薄片的数字 PCR 与 qRT-PCR 相比显示了变化(8.9% 对 46.3%) 和察觉(0.2 copies/L 对 1.1 copies/L ) 的更低的限制的一个更低的系数。在从有圣举起心肌的梗塞( STEMI )的 24 个病人和有稳定的冠的动脉疾病( CAD )的 20 个病人镇定的浆液在经皮的冠的干预(一种总线标准)以后的病人,我们使用了 qRT-PCR 和多路的基于薄片的数字 PCR 确定他们在优先的研究在 AMI 被验证了的 miRNA-21 和 miRNA-499 的浆液层次。在 STEMI, I/R 损害经由圣片断分辨率(ST-R ) 的测量被估计。基于薄片的数字 PCR 处于在稳定的 CAD 和 STEMI 组之间的 miR-21 层次的差别揭示了统计意义(118.8 copies/L 对 59 copies/L;P=0.0300 ) ,而 qRT-PCR 是不能的到达意义(136.4 copies/L 对 122.8 copies/L;P=0.2273 ) 。为 miR-499 层次,基于薄片的数字 PCR 和 qRT-PCR 揭示了稳定的 CAD 和 STEMI 组之间的统计上重要的差别(2 copies/L 对 8.5 copies/L, P=0.0011;0 copies/L 对 19.4 copies/L;P < 0.0001 ) 。在 miR-21/499 层次和 ST-R 之间没有协会一种总线标准以后。我们的结果证明基于薄片的数字 PCR 展出优异技术质量和诺言是一个优异方法因为确定的 miRNA 在发行量铺平,它可以为直接确定成为一个更精确、可再现的方法 miRNAs,特别地为在大多中心的使用临床的试用。 | Samuel ROBINSON Marie FOLLO David HAENE Maximilian MAULER Daniela STALLMANN Lukas Andreas HEGER Thomas HELBING Daniel DUERSCHMIED Karlheinz PETER Christoph BODE Ingo AHRENS Marcus HORTMANN | 2018 | Acta Pharmacologica Sinica2018，39，7： | 5 |
| 3 | Mobile NMR for geophysical analysis and materials testing显示文摘Initiated by well logging NMR,portable NMR instruments are being developed for a variety of novel applications in materials testing,process analysis and control,which provides new opportunities for geophysical investigations.Small-diameter cylindrical sensors can probe short distances into the walls of slim-line logging holes,and single-sided sensors enable non-destructive testing of large objects.Both sensors are characterized by small sensitive volumes.Barrel-shaped magnets that accommodate the sample in their center have higher sensitivity due to a larger sensitive volume but can accommodate only samples like drill cores,which fit in size to the diameter of the magnet bore.Both types of magnets can be scaled down to the size of a coffee mug to arrive at sub-compact NMR equipment.Portable NMR magnets are reviewed in the context of applications related to geophysics. | BLüMICH Bernhard MAULER Jrg HABER Agnes PERLO Juan DANIELI Ernesto CASANOVA Federico | 2009 | Petroleum Science2009，6，1： | 2 |
| 4 | Linear low-density polyethylene synthesis promoted by homogeneous and supported catalysts 显示文摘 | Galland G B Sefein M Mauler R S | 1999 | Polym Int1999，48，8： | 1 |
| 5 | Proceedings of IUARSS'94,Pasadena显示文摘 | Bach M Mauler W | 1994 | IEEE1994，4，： | 1 |
| 6 | Bridged lamellae: crystal structure(s) of low molecular weight linear polyethylene 显示文摘 | Galland G B Mauler R S Nunes F F | 1999 | Macromolecules1999，32，： | 1 |
| 7 | P-wave anisotropy in eclogites and relationship to the omphacite crystallographic fabric显示文摘 | Mauler A Burlini L Kunze K | 2000 | Physics and Chemistry of the Earth2000，25，： | 1 |
| 8 | Biphasic ethylene polymerization with a diimine nickel catalyst显示文摘 | Mauler R S de Souza R F | 2001 | Macromol Rapid Commun2001，22，6： | 1 |
| 9 | Regional chemotherapy of gastro-intestinal cancer 显示文摘 | Mauler CA Bomer M Buchler MW | 1977 | Digestive Surgery1977，14，： | 1 |
| 10 | Inhibition of evoked glutamate release by the neuroprotective 5-HT (1A) receptor agonist BAY x 3702 in vitxo and in vivo 显示文摘 | Mauler F Fahrig T Horvath E | 2001 | Brain Res2001，888，1： | 1 |
| 11 | Brand Community Members as a Source of Innovation 显示文摘 | Fuller J Mauler K Hoppe M | 2008 | Journal of Product Innovation Manage- ment2008，25，： | 1 |
| 12 | A factorial design applied to polypropylene functionalization with maleic anhydride显示文摘 | Nachtigall S M B Baumhardtneto R Mauler A R S | 1999 | Polymer Engineering and Science1999，39，4： | 1 |
| 13 | Crosslinking Kinetics of SBR Composites Containing Vulcanized Ground Scraps as Filler显示文摘 | Carli L N Bianehi O Mauler R S | 2011 | Polymer Bulletin2011，67，： | 1 |
| 14 | Characterization of the diarylether sulfonylester(-)-(R)-3-(2-hydroxymethylindanyl-4-oxy)phenyl-4,4,4-trifluoro-l-sulf nate(BAY 38-7271) as a potent cannabinoid receptor agonia with neuroprotective properties显示文摘 | Mauler F Miaendorf J De Vry J | 2002 | Journal of Pharmacology and Experimental Therapeutics2002，，01： | 1 |
| 15 | Inhibition of evoked glutamate release by the neuroprotective 5-HT(1A) receptor agonist BAY x 3702 in vitro and in vivo显示文摘 | Mauler F Fahrig T Horvath E | 2001 | Brain Res2001，888，1： | 1 |
| 16 | A factorial design applied to polypropylene functionalization with maleic anhydride显示文摘 | Nachtigall S M B Baumhardtneto R Mauler A | 1999 | Polymer Engineering and Science1999，39，4： | 1 |
| 17 | Risk factors and determinants of neurodevelopmental outcome in cystic periventricular leucomala- cia显示文摘 | Resch B Vollaard E Mauler U | 2000 | Eur J Pediatr2000，159，9： | 1 |
| 18 | Minimally invasive approach and surgical procedures in the lumbar spine显示文摘 | Ogon M Mauler H Wimmer C | 1997 | Orthopade1997，26，6： | 1 |
| 19 | Comparison of visual performance with blue light-filtering and ultraviolet light-filtering intraocular lenses显示文摘 | Neumaier-Ammerer B Felke S Hagen S Haas P Zeiler F Mauler H | | 0，，12： | 1 |
| 20 | Water pollution and human health in China显示文摘 | Wu C Mauler C Wang Y | 1999 | Environmental Health Perspectives1999，107，4： | 1 |
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