|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Spatial assessment of fishing effort around European marine reserves: Implications for successful fisheries management显示文摘 | STELZENMULLER V MAYNOU F BERNARD G | 2008 | Marine Pollution Bulletin2008，56，12： | 1 |
| 2 | Spatial assessment of fishing effort around European marine reserves: Implications for successful fisheries management 显示文摘 | STELZENMULLER V MAYNOU F BERNARD G | 2008 | Marine Pollution Bulletin2008，56，12： | 1 |
| 3 | Variations of the radial artery in man显示文摘 | DRIZENKO A MAYNOU C MESTDAGH H etal | 2000 | SurgRadiol Anat2000，22，： | 1 |
| 4 | Spatial assessment of fishing effort around European marine reserves:Implications for successful fisheries management显示文摘 | Stelzenmüller V Maynou F Bernard G | 2008 | Marine Pollution Bulletin2008，56，12： | 1 |
| 5 | Is surgical treatment of deltoidligament rupture necessary in ankle fractures显示文摘 | Maynou C Lesage P Mesidgh H | 1997 | Rev Chir OrthopReparatrice Appar Mot1997，83，： | 1 |
| 6 | Variations of the radial artery in man显示文摘 | Drizenko A Maynou C Mestdagh H | 2001 | Chin J Clin Anat2001，19，4： | 1 |
| 7 | Spatial assessment of fishing effort around European marine reserves: Implications for successful fisheries management 显示文摘 | STELZENMULLER V MAYNOU F BERNARD G | 2008 | Marine Pollution Bulletin2008，56，： | 1 |
| 8 | Clinical results of arthroscopic tenotomy of the long head of the biceps brachii in full thickness tears of the rotator cuff without repair:40 cases显示文摘 | Maynou C Mehdi N Cassagnaud X | 2005 | Rev Chir Orthop Reparatrice Appar Mot2005，91，4： | 1 |
| 9 | Spatial assessment of fishing effort around European marine reserves: Implications for successful fisheries management显示文摘 | Stelzenmüller V Maynou F Bernard G | 2008 | Marine Pollution Bulletin2008，56，12： | 1 |
| 10 | A subspace method for the detection of transcription factor binding sites 显示文摘 | PairoE Maynou J Marco S | 2012 | Bioinformatics2012，，： | 1 |
| 11 | Computational Detection of Tran- scription Factor Binding Sites Through Differential R6nyi Entropy 显示文摘 | MAYNOU J GALLARDO - CHACON J J VALLVERDU M CAMINAL P PERERA A | 2010 | Information Theory IEEE Transactions on2010，56，2： | 1 |
| 12 | The function of the sub- scapularis after surgical treatment for recurrent instability of the shoulder using a bone block procedure 显示文摘 | Maynou C Cassagnaud X Mestdagh H | 2005 | J Bone Joint Surg Br2005，87，8： | 1 |
| 13 | Bathymetric changes in the distributions of particulate organic matter and associated fauna along a deep-sea transect down the Catalan Sea slope (Northwestern Mediterranean) 显示文摘 | Carts J E Grémare A Maynou F | 2002 | Progress in Oceanography2002，53，： | 1 |
| 14 | Effect of the rotator cuff condition on the results of shoulder arthroplasty显示文摘 | Mestdagh H Petroff E Maynou C | 1997 | Rev Chir Orthop Reparatrice Appar Mot1997，83，6： | 1 |
| 15 | Effect of the rotator cuff condition on the results of shoulder arthroplasty显示文摘 | Mestdagh H Petroff E Maynou C | | 0，，06： | 1 |
| 16 | Analysis of catch per unit effort by multivariate analysis and generalized linear models for deep-water crustacean fisheries off Barcelona ( NW Mediterranean ) 显示文摘 | Maynou F Demestre M Sfinchez P | 2003 | Fisheries Research2003，65，1： | 1 |
| 17 | Long term results of the surgical treatment of bicondylar fractures of the distal humerus extremity in adults显示文摘 | Elhage R Maynou C Jugnet PM | 2001 | Chir Main2001，20，2： | 1 |
| 18 | Determination of double-and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity显示文摘Background:The analysis of chromatin integrity has become an important determinant of sperm quality.In frozenthawed bovine sperm,neither the sequence of post-thaw injury events nor the dynamics of different types of sperm DNA breaks are well understood.The aim of the present work was to describe such sperm degradation aftermath focusing on DNA damage dynamics,and to assess if this parameter can predict pregnancy rates in cattle.Results:A total of 75 cryopreserved ejaculates from 25 Holstein bulls were evaluated at two post-thawing periods(0-2 h and 2-4 h),analyzing global and double-stranded DNA damage through alkaline and neutral Comet assays,chromatin deprotamination and decondensation,sperm motility,viability,acrosomal status,and intracellular levels of total ROS,superoxides and calcium.Insemination of 59,605 females was conducted using sperm from the same bulls,thus obtaining the non-return to estrus rates after 90 d(NRR).Results showed an increased rate of double-stranded breaks in the first period(0-2 h:1.29±1.01%/h vs.2-4 h:0.13±1.37%/h;P<0.01),whereas the rate of sperm with moderate+high single-stranded breaks was higher in the second period(0-2 h:3.52±7.77%/h vs.2-4h:21.06±11.69%/h;P<0.0001).Regarding sperm physiology,viability decrease rate was different between the two periods(0-2 h:-4.49±1.79%/h vs.2-4 h:-2.50±3.39%/h;P=0.032),but the progressive motility decrease rate was constant throughout post-thawing incubation(0-2 h:-4.70±3.42%/h vs.2-4 h:-1.89±2.97%/h;P>0.05).Finally,whereas no correlations between bull fertility and any dynamic parameter were found,there were correlations between the NRR and the basal percentage of highly-damaged sperm assessed with the alkaline Comet(Rs=-0.563,P=0.003),between NRR and basal progressive motility(Rs=0.511,P=0.009),and between NRR and sperm with high ROS at 4 h post-thaw(Rs=0.564,P=0.003).Conclusion:The statistically significant correlations found between intracellular ROS,sperm viability,sperm motility,DNA damage and chromatin deprotamination suggested a sequence of events all driven by oxidative stress,where viability and motility would be affected first and sperm chromatin would be altered at a later stage,thus suggesting that bovine sperm should be used for fertilization within 2 h post-thaw.Fertility correlations supported that the assessment of global DNA damage through the Comet assay may help predict bull fertility. | Jordi Ribas‑Maynou Ariadna Delgado‑Bermúdez Yentel Mateo‑Otero Estel Vinolas Carlos O.Hidalgo WSteven Ward Marc Yeste | 2023 | Journal of Animal Science and Biotechnology2023，14，1： | 0 |
| 19 | Expression of miR‑138 in cryopreserved bovine sperm is related to their fertility potential显示文摘Background MicroRNAs(miRNAs)are small,single-stranded,non-coding RNA molecules of 22–24 nucleotides that regulate gene expression.In the last decade,miRNAs have been described in sperm of several mammals,including cattle.It is known that miRNAs can act as key gene regulators of early embryogenesis in mice and humans;however,little is known about the content,expression,and function of sperm-borne miRNAs in early bovine embryo.In this study,total sperm RNA was isolated from 29 cryopreserved sperm samples(each coming from a separate bull)using a RNeasy kit and treatment with DNase I.RNA concentration and purity were determined through an Epoch spectrophotometer and an Agilent Bioanalyzer.The expression of 10 candidate miRNAs in bovine sperm(bta-miR-10a,bta-miR-10b,bta-miR-138,bta-miR-146b,bta-miR-19b,bta-miR-26a,bta-miR-34a,bta-miR-449a,bta-miR-495 and btamiR-7),previously identified in testis and/or epididymis,was evaluated with RT-qPCR.The cel-miR-39-3p was used as a spike-in exogenous control.Nonparametric Mann–Whitney tests were run to evaluate which miRNAs were differentially expressed between bulls with high fertility[HF;non-return rates(NRR)ranging from 39.5 to 43.5]and those with subfertility(SF;NRR ranging from 33.3 to 39.3).Several sperm functionality parameters(e.g.,viability,membrane stability or oxygen consumption,among others)were measured by multiplexing flow cytometry and oxygen sensing technologies.Results RNA concentration and purity(260/280 nm ratio)(mean±SD)from the 29 samples were 99.3±84.6 ng/μL and 1.97±0.72,respectively.Bioanalyzer results confirmed the lack of RNA from somatic cells.In terms of the presence or absence of miRNAs,and after applying the Livak method,8 out of 10 miRNAs(bta-miR-10b,-138,-146b,-19b,-26a,-449a,-495,-7)were consistently detected in bovine sperm,whereas the other two(bta-miR-10a,and-34a)were absent.Interestingly,the relative expression of one miRNA(bta-miR-138)in sperm was significantly lower in the SF than in the HF group(P=0.038).In addition to being associated to fertility potential,the presence of this miRNA was found to be negatively correlated with sperm oxygen consumption.The expression of three other miRNAs(bta-miR-19b,bta-miR-26a and bta-miR-7)was also correlated with sperm function variables.Conclusions In conclusion,although functional validation studies are required to confirm these results,this study suggests that sperm bta-miR-138 is involved in fertilization events and beyond,and supports its use as a fertility biomarker in cattle. | Albert Salas‑Huetos Jordi Ribas‑Maynou Yentel Mateo‑Otero Carolina Tamargo Marc Llavanera Marc Yeste | 2023 | Journal of Animal Science and Biotechnology2023，14，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员