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| 1 | In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene显示文摘We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells. | JIAN MIN YANG MICHAEL S FRIEDMAN MARIANNE T HUBEN JENNIFER FULLER QIAO LI ALFRED E CHANG JAMES J MULE KEVIN T MCDONAGH | 2006 | Journal of Microbiology and Immunology2006，4，2： | 3 |
| 2 | 转染嵌合性T细胞受体基因小鼠T淋巴细胞的体外抗肿瘤作用显示文摘目的:研究小鼠T淋巴细胞在转染嵌合性T细胞受体基因后的体外抗肿瘤作用。方法:应用重组DNA技术,将HER2特异性嵌合性T-细胞受体构建入逆转录病毒载体;转入包装细胞后,收集病毒上清,转染小鼠T淋巴细胞,转基因后的小鼠T淋巴细胞分别与HER2阳性(SK-OV-3)或阴性(MCF-7)的肿瘤细胞系共培养,检测其细胞因子γ干扰素释放,51Cr释放法检测CTL评价其抗肿瘤效应。结果:所构建载体经酶切鉴定符合要求,乒乓法转染包装细胞系GP+E86,检测病毒滴度为1.2×106,Retronectin结合离心法转染经抗CD3/CD28单抗活化的小鼠T淋巴细胞,转染效率可达50%以上;转染嵌合性T细胞受体基因的T淋巴细胞与HER2阳性或阴性的肿瘤细胞系共培养后可检测到HER2特异性的细胞因子γ干扰素释放,51Cr释放法测CTL可见转染嵌合性T细胞受体基因T淋巴细胞对HER2阳性的肿瘤细胞具显著杀伤效应。结论:转染嵌合性T细胞受体基因的小鼠T淋巴细胞在体外可通过细胞因子释放和CTL效应发挥显著的抗肿瘤作用。 | 杨建民 Michael S Friedman 李峤 James J Mule Alfred E Chang Kevin T McDonagh | 2006 | 中国肿瘤生物治疗杂志2006，13，4： | 2 |
| 3 | Left ventricular dysfunction, natriuretic peptide, and mortality in an urban population 显示文摘 | McDONAGH T CUNNINGHAM A D MORRISON C E | 2001 | Heart2001，86，： | 1 |
| 4 | Chem Phys Lett显示文摘 | Yuk J S O'Reilly E Forster R J MaeCraith B D McDonagh C | 2011 | 513:ll22011，513，： | 1 |
| 5 | Viral and bacterial pathogens at the maternal-fetal interface显示文摘 | McDonagh S Maidji E Ma W | 2004 | J Infect Dis2004，190，4： | 1 |
| 6 | Patterns of human cytomegalovirus infection in term placentas:a preliminary analysis显示文摘 | McDonagh S Maidji E Chang HT | 2006 | Clin Virol2006，35，2： | 1 |
| 7 | Human cytomegalovirus transmission from the uterus to the placenta correlates with the presence of pathogenic bacteria and maternal immunity显示文摘 | Pereira L Maidji E McDonagh S | 2003 | J Virol2003，77，13： | 1 |
| 8 | Maternal antibodies enhance or prevent cytomegalovirus infection in the placenta by neonatal Fc receptor-mediated transcytosis显示文摘 | Maidji E McDonagh S Genbacev O | 2006 | Am J Pathol2006，168，： | 1 |
| 9 | The impact of a coordinated transitional care programme on adolescents with juvenile idiopathic arthritis显示文摘 | McDonagh J E Southwood T R Shaw K L | | 0，，： | 1 |
| 10 | Engineered antibody-drug conjugates with defined sites and stoichiometries of drug attachment 显示文摘 | MCDONAGH CF TURCOTT E WESTENDORF L | 2006 | Protein Eng Des Sel2006，19，7： | 1 |
| 11 | Insights into viral transmission at the uterine placental interface显示文摘 | Pereira L Maidji E McDonagh S | 2005 | Trends Microbiol2005，13，4： | 1 |
| 12 | Pharmacogenomics knowledge for personalized medicine 显示文摘 | Whirl-Carrillo M McDonagh E M Hebert J M | 2012 | clinical pharmacology and therapeutics tics2012，92，4： | 1 |
| 13 | On the export of Antaretic Bottom Water from the Weddell Sea显示文摘 | Naveira Garabato A C McDonagh E L Stevens D P | 2002 | Deep Sea Research Part II: Topical Studies in Oceanography2002，49，21： | 1 |
| 14 | Modeling and simulation of wireless sensor system for health monitoring using HDL and Simulink mixed environment显示文摘 | Arshak K Jafer E McDonagh D | 2007 | IET Computers and Digital Techniques2007，1，5： | 1 |
| 15 | Development of additional autoimmunediseases in a population of patients with systemic lupus erythematosus 显示文摘 | Mcdonagh J E Isenberg D A | 2000 | Ann Rheum Dis2000，59，3： | 1 |
| 16 | Changes in ocean water mass properties: oscillations of trends显示文摘 | Bryden H L McDonagh E King B A | 2003 | Science2003，300，： | 1 |
| 17 | Symptomatic and asymptomatic left-ventricular Systolic dysfunction in an urban population 显示文摘 | McDonagh T A Morrison C E Lawrence A | 1997 | Lancet1997，，350： | 1 |
| 18 | The impact of a coordinated transitional care programme on adoles- cents with juvenile idiopathic arthritis显示文摘 | McDonagh J E Southwood T R Shaw K L | 2007 | Rheumatology (Oxford)2007，46，1： | 1 |
| 19 | Decadal changes inthe south Indian ocean thermocline显示文摘 | McDonagh E L Bryden H L King B A | 2005 | Journal of Climate2005，18，10： | 1 |
| 20 | The impact of a coordinated transitional care programme on adolescents with juvenile idiopathic arthrI tis显示文摘 | McDonagh J E Southwood T R Shaw K L | 2007 | Rheumatology (Oxford)2007，46，1： | 1 |
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