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| 1 | Increased expression of tumor necrosis factor-a is associated with advanced colorectal cancer stages显示文摘AIM:To detect the expression of tumor necrosis factor-a(TNF-a)in colorectal cancer(CRC)cells among Saudi patients,and correlate its expression with clinical stages of cancer.METHODS:Archival tissue specimens were collected from 30 patients with CRC who had undergone surgical intervention at King Khalid University Hospital.Patient demographic information,including age and gender,tumor sites,and histological type of CRC,was recorded.To measure TNF-a m RNA expression in CRC,total RNA was extracted from tumor formalin-fixed,paraffinembedded,and adjacent normal tissues.Reverse transcription and reverse transcription polymerase chain reaction were performed.Colorectal tissue microarrays were constructed to investigate the protein expression of TNF-a by immunohistochemistry.RESULTS:The relative expression of TNF-a m RNA in colorectal cancer was significantly higher than that seen in adjacent normal colorectal tissue.High TNF-a gene expression was associated with StageⅢandⅣneoplasms when compared with earlier tumor stages(P=0.004).Eighty-three percent of patients(25/30)showed strong TNF-a positive staining,while only 10%(n=3/30)of patients showed weak staining,and 7%(n=2/30)were negative.We showed the presence of elevated TNF-a gene expression in cancer cells,which strongly correlated with advanced stages of tumor.CONCLUSION:High levels of TNF-a expression could be an independent diagnostic indicator of colorectal cancer,and targeting TNF-a might be a promising prognostic tool by assessment of the clinical stages of CRC. | Omar A Al Obeed Khayal A Alkhayal Abdulmalik Al Sheikh Ahmad M Zubaidi Mansoor-Ali Vaali-Mohammed Robin Boushey James H Mckerrow Maha-Hamadien Abdulla | 2014 | World Journal of Gastroenterology2014，20，48： | 8 |
| 2 | Proteases in parasitic diseases显示文摘 | McKerrow J H Caffrey C Kelly B | 2006 | Annu Rev Pathol2006，1，： | 1 |
| 3 | Cysteine proteases of parasitic organisms 显示文摘 | Sajid M McKerrow JH | 2002 | Mol Biochem Parasitol2002，120，： | 1 |
| 4 | A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval mohing and cuticle and eggshell remodeling 显示文摘 | Guiliano DB Hong X McKerrow JH eta/ | 2004 | Mol Biochem Parasitol2004，136，2： | 1 |
| 5 | Development of cysteine protease inhibitors as chemotherapy for parasitic diseases: insights on safety, target validation,and mechanism of action显示文摘 | McKerrow JH | 1999 | Int J Parasitol1999，29，： | 1 |
| 6 | Schistosoma mansoni: sex-specific modulation of parasite growth by host immune signals显示文摘 | Hemandez DC Lim KC McKerrow JH | 2004 | Exp Parasitol2004，106，12： | 1 |
| 7 | Parasite proteases 显示文摘 | McKERROW J H | 1986 | Exp Parasitol1986，68，1： | 1 |
| 8 | Regulation of the Caenorhabditis elegans gut cysteine protease gene cpr-l: requirement for GATA motifs显示文摘 | Britton C Mckerrow J H Johnstone I L | 1998 | Journal of Molecular Bi- ology1998，283，1： | 1 |
| 9 | Production of crystallizable cruzain,the major cysteine protease from Trypanosoma cruzi显示文摘 | McGrath ME McKerrow JH | 1993 | J Biol Chem1993，268，9： | 1 |
| 10 | Gut-specific and developmental ex- pression of a Caenorhabditis elegans cysteine protease gene显示文摘 | Ray C McKerrow J H | 1992 | Molecular and Biochemical Parasitology1992，51，2： | 1 |
| 11 | Gondwanan palaeogeography and palaeoclimatology显示文摘 | Scotese C R Boucot A J Mckerrow W S | 1999 | Journal of African Earth Sciences1999，28，1： | 1 |
| 12 | Design,synthesis and evaluation of d-homophe- nylalanyl epoxysuccinate inhibitors of the trypanosomal cysteine protease cruzain显示文摘 | Hernandez A A McKerrow | 2000 | Tetrahedron2000，56，50： | 1 |
| 13 | Hormone receptors in hepatoblastoma: a demonstration of both estrogen and progesterone receptors显示文摘 | Demans DJ Friedman MA McKerrow JH | 1982 | Cancer1982，50，： | 1 |
| 14 | Cysteine proteases of parasitic organisms 显示文摘 | Sajid M Mckerrow JH | 2002 | Mol Biochem Parasitol2002，120，1： | 1 |
| 15 | Critical rhetoric: Theory and praxis显示文摘 | Raymie E. Mckerrow | 1989 | Communication Monographs1989，，2： | 1 |
| 16 | A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval molting and cuticle and egg显示文摘 | Guilianoa DB Hong X Mckerrow JH | 2004 | Mol Biochem Parasitol2004，136，2： | 1 |
| 17 | Cysteine protease inhibitors as chemotherapy for parasitic infections显示文摘 | McKerrow JH Engel JC Caffrey CR | 1999 | Bioorg Med Chem1999，7，： | 1 |
| 18 | Proteases in parasitic dis- eascs显示文摘 | Mckerrow JH Caffrey C Kelly B | 2006 | Annu Rev Pathol2006，1，： | 1 |
| 19 | Regulation of Trichophyton rubrum proteolytic activity显示文摘 | Apodaca G McKERROW JH | 1989 | Infection and Immunity1989，57，10： | 1 |
| 20 | Paleozoic paleogeography显示文摘 | Ziegler A M Scotese C R McKerrow M S etal | 1979 | Annual Review of Earth and Planetary Sciences1979，7，： | 1 |
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