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| 1 | 热休克预处理提高猪成肌细胞自体正常肌肉移植后的生存率显示文摘研究表明成肌细胞移植有可能用于治疗Duchenne型肌营养不良(Duchenne muscular dystrophy,DMD)、心衰和严重肌肉损伤.移植后成肌细胞快速大量的死亡是影响该方法疗效的一个主要难题.细胞在受到各种刺激后会过度表达热休克蛋白(heat shock protein,HSP).体外和体内实验均已证实HSP能帮助细胞对抗多种有害刺激.本实验针对HSP的过度表达能否提高猪成肌细胞自体正常肌肉移植后的存活率进行研究.猪成肌细胞经过42℃,1h的热休克后会过度表达HSP.通过Western blot和流式细胞仪来定量检测猪成肌细胞中HSP的表达;同时分析细胞中的CD56和结合蛋白含量,从而了解休克后细胞的肌源性有无变化.利用放射性H3定量分析移植后细胞的生存率.借助慢病毒载体(Lentivector LacZ)监测移植后成肌细胞与宿主细胞的融合情况.结果表明热休克预处理后成肌细胞的HSP70显著增高(P<0.01),并且细胞的肌源性无变化.成肌细胞体内移植后(n=7),24h时热休克组和非热休克组的细胞存活率无显著性差异((67.69±8.35)%vs(58.79±8.35)%,P>0.05).48h时热休克组细胞存活率比非热休克组的高2倍((53.32±8.22)%vs(28.27±6.32)%,P<0.01).120h时热休克组细胞存活率比非热休克组的高3倍((26.33±5.54)%vs(8.79±2.51)%,P<0.01).组织学检查显示;热休克细胞和非热休克细胞均能与宿主细胞融合.这些结果提示热休克预处理能增强成肌细胞HSP70的表达,并可提高成肌细胞自体移植后的生存率.体外热休克预处理是一个简单有效的能改善猪成肌细胞自体移植生存率的方法.这也许是一个解决成肌细胞移植后存活率低的方法. | 杨圣 Laumonier Thomas Menetrey Jacques | 2007 | 中国科学(C辑)2007，37，3： | 3 |
| 2 | Triaxial failure criterion for concrete and its generalization 显示文摘 | MENETREY P WILLIAM K J | 1995 | Structural Journal1995，92，3： | 1 |
| 3 | Suturing versus immobilization of a muscle laceration:a morphological and functional study显示文摘 | Menetrey J Kasemkijwattana C Fu FH | 1999 | Am J Sports Med1999，27，2： | 1 |
| 4 | Synthesis of punching failure in reinforced concrete 显示文摘 | MENETREY P | 2002 | Cement Concrete Comp2002，2，2： | 1 |
| 5 | Growth factors improve muscle healing in vivo显示文摘 | Menetrey J Kasemkijwattana C Day CS | 2000 | Journal of Bone and Joint Surgery2000，82，1： | 1 |
| 6 | Use of growth factors to improve muscle healing after strain injury显示文摘 | Kasemkijwattana C Menetrey J Bosch P | 2000 | Clinical Orthopaedics and Related Research2000，370，： | 1 |
| 7 | Growth factors improve muscle healing in vivo显示文摘 | Menetrey J Kasemkijwattana C Day CS | 2000 | J Bone Joint Surg Br2000，82，1： | 1 |
| 8 | Use of growth factors to improve muscle healing after strain injury显示文摘 | Kasemkijwattana C Menetrey J Bosch P | 2000 | Clin Orthop Relat Res2000，370，： | 1 |
| 9 | a-Smooth muscle actin and TGF-β receptor I expression in the healing rabbit medial collateral and anterior cruciate ligaments 显示文摘 | Menetrey J Laumonier T Garavaglia G | 2011 | Injury2011，42，： | 1 |
| 10 | Non-hematopoietic human bone marrow contains long-lasting,pluripotential esenchymal stem cells显示文摘 | Suva D Garavaglia G Menetrey J | 2004 | J Cell Physiol2004，198，1： | 1 |
| 11 | Non-hematopoietic human bone marrow contains long-lasting, pluripotential mesenchymal stem cells显示文摘 | Suva D Garavaglia G Menetrey J | 2004 | J Cell Physiol2004，198，1： | 1 |
| 12 | Spinal and trigeminal projections to the nucleus of the solitary tract: a possible substrate for somatovisceral and viscerovisceral reflex activation 显示文摘 | MENETREY D BASBAUM A I | 1987 | J Comp Neurol1987，255，： | 1 |
| 13 | Systemic morphine suppresses noxious stimulus-evoked Fos protein-like immunoreactivity in the rat spinal cord 显示文摘 | Presley RW Menetrey D Levine JD | 1990 | J Neurosci1990，10，： | 1 |
| 14 | Application of bovine microsatellite markers for genetic diversity anal- ysis of Swiss yak (Poephagus grunniens) 显示文摘 | Nguyen T T Genini S Menetrey F | 2005 | Anita Genet2005，36，： | 1 |
| 15 | Development of approaches to improve the healing following muscle contusion显示文摘 | Kasemkijwattana C Menetrey J Somogyl G | 1998 | Cell Transplant1998，7，6： | 1 |
| 16 | Growth factors improve muscle healing in vivo显示文摘 | Menetrey J Kasemkijwattana C Day CS | 2000 | J Bone Joint Surg Br2000，82，1： | 1 |
| 17 | Non - hematopoietic human bone marrow contains long - lasting, pluripotential mesenchymal stem cells显示文摘 | Suva D Garavaglia G Menetrey J | 2004 | J Cell Physiol2004，198，： | 1 |
| 18 | Cortical oedema:a link between delusional misidentification syndromes and hemiplegic migraine显示文摘 | Moreira T Menetrey A Carota A | 2010 | J Neurol Neurosurg Psychiatry2010，81，： | 1 |
| 19 | Systemic morphinesuppresses noxious stimulus evoked Fos protein-like immu-noreactivity in the rat spinal cord 显示文摘 | Presley RW Menetrey D Levine JD | 1990 | J Neurosci1990，10，1： | 1 |
| 20 | Non-hematopoietic human bone marrow contains long-lasting,pluripotential mesenchymal stem cel s显示文摘 | Suva D Garavaglia G Menetrey J | | 0，，01： | 1 |
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