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| 1 | Modeling mixed-mode fracture propagation in isotropic elastic three di- mensional solid显示文摘 | Meng Chunfang Frantz Maerten David D Pollard | 2013 | International Journal of Fracture2013，179，12： | 1 |
| 2 | Impact of apolipoprotein A1 on tumor immune microenvironment, clinical prognosis and genomic landscape in hepatocellular carcinoma显示文摘Background:Current knowledge on apolipoprotein A1(APOA1)in hepatocellular carcinoma(HCC)is fragmented and even contradic-tory.Multi-dimensional analyses are required to comprehensively elucidate its value and underlying mechanism.Methods:We collected 49 RNA-seq datasets,40 cell line types data and 70 scRNA pan-cancer datasets public available,including 17 HCC datasets(1754 tumor samples),and enrolled 73 pairs of HCC tissue and 516 blood samples independently from our clinics.APOA1 impacting on the HCC tumor microenvironment(TME)was analyzed using intensive data mining.Methylation sequencing,flow cy-tometry,quantitative PCR,western blot,immunohistochemistry and clinical chemistry assays were conducted for wet experimental investigation.Results:The APOA1 ontology fingerprint indicated that it played various crucial biological roles in HCC,primarily involved in choles-terol efflux.Consistent findings at histology,serology,and clinical follow-up revealed that high APOA1 was a good prognosis indicator of HCc.Hypermethylation in the APOA1 promoter region was found in clinical samples which is in accordance with the reduction of APOA1 in HCC.The cell cycle,DNA replication,mismatch repair pathways,and tumor cell proliferation were less observed in the HCC APOAihigh subgroup.The favorable immunoregulatory abilities of APOA1 showed interesting findings:a positive correlation between APOA1 and anti-tumor immune cells(NK,CD8+T cells)and a negative association with immune cells exerting immunosuppressive effects,including M2 macrophages.Conclusion:This is an integrative multidimensional exploration of APOA1 using bioinformatics and experiments.Both the prognostic value and anti-tumor effects based on APOA1 panoramic exploration in the HCC TME demonstrate a new potential clinicai target for HCC assessment and intervention in the future. | Ying Wang Shipeng Chen Xiao Xiao Fan Yang Jinhan Wange Hui Zong Yuzhen Gao Chenjun Huang Xuewen Xu Meng Fang Xiaoyan Zhang Chunfang Gao | 2023 | Precision Clinical Medicine2023，6，3： | 0 |
| 3 | Establishment of a real-time PCR for quantifying transforming growth factor beta1 in blood of hepatocellular carcinoma patients显示文摘Background: The carcinogenesis of hepatocellular carcinoma (HCC) is a multi-factorial, multistep and complex process. Its prognosis is poor and early detection is of the utmost importance. Transforming growth factor β1 (TGF-β1) message RNA (mRNA) has been reported to be elevated in HCC patients using Northern blotting. However, little work has been done about the detection of TGF-β1 mRNA levels in peripheral blood of patients with HCC using the real-time polymerase chain reactions (PCR) method. Objective: To assess the prognostic value of quantitative levels of TGF-β1 mRNA in peripheral blood of patients with HCC, and to investigate the relationship between the expression of TGF-β1 mRNA in peripheral blood and many diagnostic and pathological factors. Methods: We developed an optimized Taqman real-time PCR to quantify TGF-β1 mRNA in peripheral blood of 53 patients with HCC and 44 healthy volunteers. In addition, blood was collected from patients with HCC for measuring levels of total bilirubin (TBil), prealbumin, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyltranspeptidase (GGT), alpha-L-fucosidase (AFU), alpha fetoprotein (AFP), carcino-embryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), viral load and platelet counts. Statistical analysis was performed using the SPSS software system (SPSS 10.0). Results: In real-time PCR, fluorescence was detectable in all blood specimens from patients with HCC and healthy volunteers. The levels of TGF-β1 mRNA expression in patients with HCC were significantly higher compared to that in healthy volunteers (P<0.000 1), suggesting an association of the activated TGF-β1 gene transcription with hepato- carcinogenesis. Patients with HCC were divided into 2 groups according to their TGF-β1 mRNA above (group A, n=28)or below (group B, n=25) the mean level. Statistical results demonstrated that TGF-β1 mRNA expression level was correlated with patients age, serum levels of CEA, CA19-9 and viral copy number (P<0.05). Conclusion: Although this is a small sample size pilot study these findings imply that quantitative measurement of TGF-β1 mRNA level in peripheral blood may be a complementary serologic marker of HCC. | Qi Peng Gao Chunfang Fang Meng Ji Qiang Zhao Yunpeng Liu Yan Sun Xiaojuan | 2008 | Journal of Medical Colleges of PLA(China)2008，23，4： | 0 |
| 4 | 5-31 Progress of the SMT in the Control Technology Group显示文摘The SMT group was founded in 2002, established for service HIFRL - CSR project. The main production IMPaccelerator control system independently is developed by a dedicated PCB components assembly, welding, repair,and complete sets of equipment assembly and testing. Since establishing we successful welding thousands piecesof circuit boards, including DSP, ARM, Fiber - the HUB, and the network type terminal controller, etc. Most ofproducts have been used in HIFRL - CSR project. The production line team undertakes the task of producingand welding spare parts in accelerator control system, which has been playing an important role in the acceleratormaintenance work until today. | Wang Dan Zhang Shuocheng Meng Jian Wang Chunfang Xiao Xiong | 2014 | IMP & HIRFL Annual Report2014，，1： | 0 |
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