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1篇 您的检索式：作者名="Michael PMurphy"

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		题名	作者	年代	出处	被引量
	1	Active RNA interference in mitochondria显示文摘RNA interference(RNAi)has been thought to be a gene silencing pathway present in most eukaryotic cells to safeguard the genome against retrotransposition.Small interfering RNAs(siRNAs)have also become a powerful tool for studying gene functions.Given the endosymbiotic hypothesis that mitochondria originated from prokaryotes,mitochondria have been generally assumed to lack active RNAi;however,certain bacteria have Argonaute homologs and various reports suggest the presence of specific microRNAs and nuclear genome(nDNA)-encoded Ago2 in the mitochondria.Here we report'that transfected siRNAs are not only able to enter the matrix of mitochondria;but also function there to specifically silence targeted mitochondrial transcripts.The mitoRNAi effect is readily detectable at the mRNA level,but only recordable on relatively unstable proteins,such as the mtDNA-encoded complex IV subunits.We also apply mitoRNAi to directly determine the postulated crosstalk between individual respiratory chain complexes,and our result suggests that the controversial observations previously made in patient-derived cells might result from differential adaptation in different cell lines.Our findings bring a new tool to study mitochondrial biology.	Kuanxing Gao Man Cheng Xinxin Zuo Jinzhong Lin Kurt Hoogewijs Michael PMurphy Xiang-Dong Fu Xiaorong Zhang	2021	Cell Research2021，31，2：	1

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