| 1 | The study of special killing effect of CD3AK on anti-CEA-positive tumor enhanced by DC loaded with CEA-rV显示文摘Objective: To survey the special killing activity of CD3AK on anti-CEA-positive tumor enhanced by umbilical cord blood dendritic cell (DC) loaded with CEA recombinant vaccinia virus (CEA-rV). Methods: Freshly isolated umbilical blood mononuclear cells (UBMC) were cultivated for 3 h. Suspension cells and attached cells were used to induce CD3AK cells and DC separately. DC was loaded with CEA-rV on the 3rd day to prepare CEA-rV+DC. CD3AK cells were co-cultured with CEA- rV+DC on the 8th day, to prepare CEA-rV+DC+CD3AK. The killing activity of each effector’s cell, which included UBMC, CD3AK, DC+CD3AK and CEA-rV+DC+CD3AK, was measured respectively by MTT reduction assay. Results: (1) 4 target cells were con- firmed by CEA monoclonal antibody of rabbit anti-human. Lovo and A549 were really CEA positive cell lines, while Bel-7402 and K562 were CEA negative cell lines. (2) It was showed by flow-cytometry that the mature DC cultured at 10th day expressed MHC I, II molecules such as CD86, CD80, CD83 and CD40 highly, but CD123 lowly. The expression rates of CD86, CD80, CD83 and CD40 was 82.7%, 51.1%, 57.5% and 69.4%, respectively. The appearances and intra-cellular structures of DC were observed through light and electron microscope. The diameter of mature DC was 15–20 μm presented the irregular morphologic appear- ance, much prominences and pseudopodium. There were abundant mitochondria and endoplasmic reticulum in DC endochy- lema. (3) The rates of CD3, CD4, CD8 and CD28 in CD3AK cells group were 2 folds higher than that in UBMC group by FACS. It was said that the numbers of the mature T lymphocyte in CD3AK cells group were much greater than that in UBMC group. (4) The killing activities to 4 target cells of 3 effector’s cells, which included CEA-rV+DC+CD3AK, DC+CD3AK and CD3AK, were much greater than that of UBMC (P<0.01). Moreover, comparing with the killing activities of 4 effector’s: CEA-rV+DC+CD3AK group > DC+CD3AK group > CD3AK group > UBMC group. It showed that, cytokine, DC and CEA-rV could efficiently elevate the killing activity of UBMC on broad-spectrum tumor cells. (5) Comparing with the killing activities of CEA-rV+DC+CD3AK and CD3AK cells to CEA positive and negative cells, the killing activities of CEA-rV+DC+CD3AK to CEA positive tumor cells, Lovo and A549 cells (P<0.01) were remarkably better than that to CEA negative tumor cells BEL-7402 and K562 cells (P<0.05). It was said that the CEA-rV+DC could obviously enhance the killing activity of CD3AK on CEA positive tumor cells. Comparing with the killing activities of CEA-rV+DC+CD3AK and DC+CD3AK cells, the killing activity of CD3AK on CEA negative tumor cells was no statistical difference (P>0.05). However, the killing activity to CEA positive cells of CEA-rV+DC+CD3AK group was notably higher than that of DC+CD3AK group. Namely, CEA-rV could distinctly promote the special killing activity to CEA positive tumor cells of CD3AK, but could not do it to CEA negative tumor cells. Conclusion: CEA-rV+DC could obviously enhance the special killing activity of CD3AK on CEA positive tumor cell lines, while the DC only couldn’t. The results indicated that the CEA-rV played an important role during the special killing activity of CD3AK cells to CEA positive tumor cells. | Yanchao Qi Xinshuai Wang Bo Yang Minying Lu Dongxiao Pan Feng Liu | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，6： | 1 |
| 2 | Molecular Design and Immunogenicity of a Multiple-epitope FMDV Antigen and DNA Vaccination显示文摘This article reports the design and construction of a multiple-epitope foot and mouth disease virus (FMDV) antigen, designated as OAAT. This recombinant antigen consists of the structural protein VP1 genes from serotypes A and O FMDV, five major VP1 immunodominant epitopes from two genotypes of Asia1 serotype, and three Th2 epitopes originating from the nonstructural protein, three ABC gene and structural protein VP4 gene. Expressions of target gene from these plasmids in HeLa cells were verified by Western-blot. BALB/c mice were immunized intramuscularly with the DNA vaccines thrice every two weeks. We found that pA could induce simultaneously specific antibodies against serotypes A, Asia1, and O FMDV. Compared to those of the controls, the spots of FMDV-specific IFN-γ and cytotoxic activity from mice immunized with pA were significantly increased. pA provided full protection in 2/4 guinea pigs from challenge with FMDV O/NY00 and Asia1/YNBS/58, respectively. The results show that although pA did not give full protection in 100% immunized guinea pigs from challenge with type O and Asia1 FMDV, respectively, OAAT may be potential immunogen against FMDV and pA may be potential DNA vaccines against FMDV. | MA Ming-xiao JIN Ning-yi LIU Hui-juan ZHENG Mini YIN Ge-fen TIAN Ming-yao JIN Ming-lan LU Hui-jun SHEN Guo-shun ZHU Guang-ze JIN Hong-tao JIN Kuo-shi ZHANG Ying-jiu | 2008 | Chemical Research in Chinese Universities2008，24，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员