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| 1 | Prevalence of shiga toxins(stx_1,stx_2),eaeA and hly genes of Escherichia coli O157:H7 strains among children with acute gastroenteritis in southern of Iran显示文摘Objective:To survey the prevalence severe diarrhea arising from these bacteria in children under 5 years old in Marvdasht.Methods:In this study faecal sample from 615 children aged <5years old who were hospitalized lor gastroenteritis in Fars hospitals in Iran were collected and then enriched in Escherichia coli(E.coli) broth and modified tryplone soy broth with novobiocin media,fermentation of sorbitol,lactose and β— glucoronidase activity of isolated strains was examined by CT—SMAC,VRBA and chromogenic media respectively.Then isolation of E.coli O157:H7 have been confirmed with the use of specific antisera and with multiplex PCR method presence of virulence genes including:xtx_1.stx_2,eae.A.hly has been analyzed.Results:E.coli O157:H7 was detected in 7(1.14%) stool specimens.A significanl difference was seen between detection rale of isolated bacteria from age groups 18-23 months and other age groups(P=0.004).Out of considered virulence genes.only 1 of the isolated strains(0.16%)he stx,and eaeA genes were seen and also all isolated hacleria had resistance to penicillin,ampicillin and erythromycin antibiotics.Conclusions:We found thai children < 2 years of age were at highest risk of infection with E.coli O157:H7.Regarding severity of E.coli O157:H7 pathogenesis,low infectious dose and lack of routine assay for detection ol these bacleria in clinical laboratory,further and completed studies on diagnosis and genolyping of this E.coli O157:H7 strain has been recommended. | Mohammad Kargar Maryam Homayoon | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，1： | 2 |
| 2 | FRDT: Footprint resource discovery tree for grids 显示文摘 | Leyli Mohammad Khanli Saeed Kargar | 2011 | Future Generation Computer Systems2011，27，2： | 1 |
| 3 | A simple multiplex PCR for assessing prevalence of extended-spectrumβ-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz,Iran显示文摘Objective:To identify three common genes(bla_(TEM),bla_(SHV)and bla_(CTX-M)responsible for ESBL production in Klebsiella pneumnniae(K.pneumnniae)isolated from Intensive Care Units of Namazi Hospital,Shiraz,Iran.Methods:A total of 60 non-repetitive nosocomial isolates from 60patients were selected during 2009-2010.The phenotypic identification of ESBL production was confirmed by Double Disk Synergy Test(DDST)according to CLSI guidelines.The ESBL's genotype was then analyzed by multiplex PCR of bla_(TEM),bla_(SHV)and bla_(CTX-M)genes and DNA sequencing.Results:The primary susceptibility tests of K.pneumoniae showed that among 10 examined antibiotics,the most resistant and susceptible antibiotics identified in this study were ampicillin and imipenem,respectively.The phenotypic determination of ESBL by DDST showed that 60%(n=36)of isolates produced ESBL.Multiplex PCR of genes among K.pneuunoniae isolates showed that 39%(n=18)of them have TEM,39%in=18)of then have both CTX-M and TEM and 13%(n=8)of them have TEM,SHV,CTX-M.Conclusions:Our findings reveal the high prevalence(60%)of ESBL prcxducing K.pneumnniae from ICL patients along with a new pattern of bla_(TEM)distribution differ from other countries. | Younes Ghasemi Talieh Archin Mohammad Kargar Milad Mohkam | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，9： | 0 |
| 4 | Adjuvant activity of Pasteurella multocida A strain,Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice显示文摘Objective: To evaluate the effects of Pasteurella multocida(P. multocida) vaccines on the expression and release of antibodies, interleukin(IL)-6 and IL-12 by serum. Methods: Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks. The vaccines were adjuvant with P. multocida A strain, P. multocida B strain and Salmonella typhimurium bacterial DNA(AbDNA, BbDNA and SbDNA for short, respectively). The animals were challenged 4 weeks after immunization. Blood of mice was collected to detect the change of specific antibody, IL-6, and IL-12 using ELISA. Results: The specific antibody and interleukins in the immunized group increased significantly compared to the control mice after vaccination and challenge(P<0.05). The highest release of these cytokines was obtained by P.multocida inactivated with iron and adjuvant with AbDNA at a concentration of 25 μg/mL. The antibody titer peak was 0.447 in mice vaccinated with iron-killed whole-cell antigen adjunct with AbDNA. The time-courses of release showed that bacterial DNA was able to stimulate IL-6 and IL-12 production more than alum(P<0.05). Conclusions: Our findings introduce that bacterial DNA is capable of releasing an immunological response with several cytokines.These indicate that bacterial DNA entrapped with killed P. multocida antigen is a new and effective adjuvant to enhance specific immunity and resistance of animal against the infectious pathogen, which could simplify the development of highly promising strong adjuvant. | Maryam Homayoon Yahya Tahamtan Mohammad Kargar Seyed Mohammad Hossein Hosseini Abbas Akhavan Sepahy | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，5： | 0 |
| 5 | Comprehensive AIDS prevention programs in prisons:A review study显示文摘Objective:Since prisoners may acquire AIDS and transmit HIV because of highly risky be-haviors,implementation of educational and therapeutic programs is important in decreasing the prevalence of AIDS and transmission of HIV.This study was conducted to recognize different types of interventions and compare their effectiveness regarding prevention of AIDS and its control in prisons.Methods:The ISI,PubMed,Google Scholar,databases were searched with use of“AIDS,”“prison,”“HIV,”and“incarceration”as keywords from January 2000 to March 2017,with ac-cess to free full text and English language as limitations.Related articles,including observational,epidemiological,case,and interventional studies,were extracted and those inconsistent with the study’s goals were excluded.Results:Forty-three studies were finally selected.The effectiveness of interventions related to AIDS prevention and control in prisons was studied.Conclusion:It seems that theory-based studies conducted with stakeholders participation and evaluated summatively are more successful,because they give a sense of ownership.In addition,considering prison as a setting can lead to more success of these programs. | Somayeh Zare Mehdi Kargar Jeyran Ostovarfar Mohammad Hossein Kaveh | 2018 | Family Medicine and Community Health2018，6，4： | 0 |
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