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| 1 | p16 promoter hypermethylation:A useful serum marker for early detection of gastric cancer显示文摘AIM: To determine p16 promoter hypermethylation in gastric tumoral tissue and serum samples, its impact on p16-protein expression, and correlation with clinical and histological features. METHODS: Samples were obtained from 52 histologically confirmed cases of gastric adenocarcinoma. Gastric tissue and serum of 50 age- and sex-matched individuals with normal gastroscopy and biopsy were obtained as control samples. Methylation-specific polymerase chain reaction (MSP) was used to evaluate methylation status of p16 promoter. p16-protein expression was analyzed by immunohistochemical staining on paraffin-embedded sections. RESULTS: Methylation was detected in 44.2% (23/52) of tumoral tissues. 60.9% of them were also methylated in serum, i.e., 26.9% of all patients (14/52). Methylation was not detected in tissue and sera of control samples. p16-protein expression was decreased in 61.5% of cases (32/52), and was significantly associated with promoter hypermethylation (P < 0.001). Methylation was significantly more frequent in higher pathological grades (P < 0.05). Methylation was not associated with other clinicopathological features and environmental factors including H pylori infection and smoking. CONCLUSION: p16 promoter hypermethylation is an important event in gastric carcinogenesis. It is the principle mechanism of p16 gene silencing. It is related to malignant tumor behavior. Detection of DNA methylation in serum may be a biomarker for early detection of gastric cancer. | Mohammad Reza Abbaszadegan Omeed Moaven Hamid Reza Sima Kamran Ghafarzadegan Azadeh A'rabi Mohammad Naser Forghani Hamid Reza Raziee Ali Mashhadinejad Mostafa Jafarzadeh Ehsan Esmaili-Shandiz Ezzat Dadkhah | 2008 | World Journal of Gastroenterology2008，14，13： | 50 |
| 2 | Stool-based DNA testing,a new noninvasive method for colorectal cancer screening,the first report from Iran显示文摘AIM:To detect tumor-associated DNA changes in stool samples among Iranian patients with colorectal cancer(CRC)compared to healthy individuals using BAT-26,p16 hypermethylation and long DNA markers.METHODS:Stool DNA was isolated from 45 subjects including 25 CRC patients and 20 healthy individuals using a new,fast and easy extraction method.Long DNA associated with tumor was detected using polymerase chain reaction method.Microsatellite studies were performed utilizing denaturating polyacrylamide gel to determine the instability of BAT-26.Methylation status of p16 promoter was analyzed using methylation-specific PCR(MSP).RESULTS:The results showed a significant difference in existence of long DNA(16 in patients vs 1 in controls,P < 0.001)and p16(5 in patients vs none in controls,P = 0.043)in the stool samples of two groups.Long DNA was detected in 64% of CRC patients;whereas just one of the healthy individuals was positive for Long DNA.p16 methylation was found in 20% of patients and in none of healthy individuals.Instability of BAT-26 was not detected in any of stool samples.CONCLUSION:We could detect colorectal cancer related genetic alterations by analyzing stool DNA witha sensitivity of 64% and 20% and a specificity of 95% and 100% for Long DNA and p16 respectively.A non-invasive molecular stool-based DNA testing can provide a screening strategy in high-risk individuals.However,additional testing on more samples is necessary from Iranian subjects to determine the exact specificity and sensitivity of these markers. | Mohammad Reza Abbaszadegan Alireza Tavasoli Arash Velayati Hamid Reza Sima Hassan Vosooghinia Mehdi Farzadnia Hamid Asadzedeh Mehran Gholamin Ezzat Dadkhah Azadeh Aarabi | 2007 | World Journal of Gastroenterology2007，13，10： | 14 |
| 3 | Finite element simulation of high speed machining Ti-6A1-4V alloy using modified mate- rial models 显示文摘 | Tugrul Ozel Mohammad Sima | 2010 | Transactions of NAMRI/SME2010，38，： | 1 |
| 4 | Modified Material Constitutive Models for Serrated Chip Formation Simulations and Experimental Validation in Machining of Titanium Alloy Ti- 6Al-4V 显示文摘 | Mohammad Sima Tugrul Ozel | 2010 | International Journal of Machine Tools and Manufactrue2010，50，11： | 1 |
| 5 | Evaluating and modeling the spatiotemporal pattern of regional-scale salinized land expansion in highly sensitive shoreline landscape of southeastern Iran显示文摘Taking an area of about 2.3×10~4 km^2 of southeastern Iran, this study aims to detect and predict regional-scale salt-affected lands. Three sets of Landsat images, each set containing 4 images for 1986, 2000, and 2015 were acquired as the main source of data. Radiometric, atmospheric and cutline blending methods were used to improve the quality of images and help better classify salinized land areas under the support vector machine method. A set of landscape metrics was also employed to detect the spatial pattern of salinized land expansion from 1986 to 2015. Four factors including distance to sea, distance to sea water channels, slope, and elevation were identified as the main contributing factors to land salinization. These factors were then integrated using the multi-criteria evaluation (MCE) procedure to generate land sensitivity map to salinization and also to calibrate the cellular-automata (CA) Markov chain (CA-Markov) model for simulation of salt-affected lands up to 2030, 2040 and 2050. The results of this study showed a dramatic dispersive expansion of salinized land from 7.7 % to 12.7% of the total study area from 1986 to 2015. The majority of areas prone to salinization and the highest sensitivity of land to salinization was found to be in the southeastern parts of the region. The result of the MCE-informed CA-Markov model revealed that 20.3% of the study area is likely to be converted to salinized lands by 2050. The findings of this research provided a view of the magnitude and direction of salinized land expansion in a past-to-future time period which should be considered in future land development strategies. | Mohammad SHAFIEZADEH Hossein MORADI Sima FAKHERAN | 2018 | Journal of Arid Land2018，10，6： | 1 |
| 6 | Aberrant p16 methylation, a possible epigenetic risk factor in familial esophageal squamous cell carcinoma显示文摘 | Mohammad Reza Abbaszadegan MT (ASCP) PhD Hamid Reza Raziee Kamran Ghafarzadegan Mohammad Taghi Shakeri Sima Afsharnezhad Mohammad Reza Ghavamnasiry | 2005 | International Journal of Gastrointestinal Cancer2005，，1： | 1 |
| 7 | Modified material constitutive models for serrated chip formation simulations and experimental validation in machining of titanium alloy Ti-6Al-4V显示文摘 | Mohammad Sima Tugrulzel | 2010 | International Journal of Machine Tools and Manufacture2010，50，11： | 1 |
| 8 | 显示文摘 | Mohammad S H Mir R M Sima G | 2015 | J Nanosci Nanotechnol2015，15，5： | 1 |
| 9 | What do Iranian general practitioners expect from family physician contracts?显示文摘background Family medicine has become a main prerequisite of providing primary healthcare and a main reforming strategy to ensure the delivery of efficient and high-quality health services.Aim This study aimed to investigate general practitioners’(GP)preferences regarding family physician contract.Design and setting Cross-sectional study was conducted among GPs who registered in Ministry of Health and Medical Education(MoHME)family physician plan and were working in the health network of moderately developed regions in Iran.The sample size was calculated to be 150 GPs who were randomly selected from MoHME database.Method Developed questionnaire was distributed to GPs.Results were analysed by ordinal regression model.results Study results confirmed that‘type of employer’had the most significant effect on GPs’preferences(β=0.86).Then attributes including‘allocating quota for being accepted in medical specialty’(β=0.78),‘increased length of contract’(β=0.00.42)and‘capitation payment+15%bonus’had respectively the great effects on participants’decision.Findings also revealed that a scenario of contracting with medical council was 2.4 times more likely to be chosen by GPs compared with a scenario of contracting with a medical university.Furthermore,a scenario that allocated a quota for admission to medical specialty courses was 2.18 times more probable to be preferred by them(p<0.001).Conclusion Successful implementation of family medicine requires development of suitable solutions for attracting and attaining GPs in the programme.It seems that using a variety of incentives and applying them in physicians’work contract would be helpful in this regard. | Rafat Mohebbifar Fatemeh Akbarirad Mohammad Ranjbar Sima Rafiei | 2019 | Family Medicine and Community Health2019，7，3： | 0 |
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