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| 1 | Assessment of tobacco heating system 2.4 on osteogenic differentiation of mesenchymal stem cells and primary human osteoblasts compared to conventional cigarettes显示文摘BACKGROUND Cigarette smoking(CS)is the most common method of consuming tobacco.Deleterious effects on bone integrity,increased incidence of fractures,and delayed fracture healing are all associated with CS.Over 150 of the 6500 molecular species contained in cigarette smoke and identified as toxic compounds are inhaled by CS and,via the bloodstream,reach the skeletal system.New technologies designed to develop a reduced-risk alternative for smokers are based on electronic nicotine delivery systems,such as e-cigarettes and tobacco heating systems(THS).THS are designed to heat tobacco instead of burning it,thereby reducing the levels of harmful toxic compounds released.AIM To examine the effects of THS on osteoprogenitor cell viability and function compared to conventional CS.METHODS Human immortalized mesenchymal stem cells(n=3)and primary human preosteoblasts isolated from cancellous bone samples from BG Unfall Klinik Tübingen(n=5)were osteogenically differentiated in vitro with aqueous extracts generated from either the THS 2.4“IQOS”or conventional“Marlboro”cigarettes for up to 21 d.Cell viability was analyzed using resazurin conversion assay(mitochondrial activity)and calcein-AM staining(esterase activity).Osteogenic differentiation and bone cell function were evaluated using alkaline phosphatase(AP)activity,while matrix formation was analyzed through alizarin red staining.Primary cilia structure was examined by acetylatedα-tubulin immunofluorescent staining.Free radical production was evaluated with 2’,7’-dichlorofluoresceindiacetate assay.RESULTS Our data clearly show that THS is significantly less toxic to bone cells than CS when analyzed by mitochondrial and esterase activity(P<0.001).No significant differences in cytotoxicity between the diverse flavors of THS were observed.Harmful effects from THS on bone cell function were observed only at very high,non-physiological concentrations.In contrast,extracts from conventional cigarettes significantly reduced the AP activity(by two-fold)and matrix mineralization(four-fold)at low concentrations.Additionally,morphologic analysis of primary cilia revealed no significant changes in the length of the organelle involved in osteogenesis of osteoprogenitor cells,nor in the number of ciliated cells following THS treatment.Assessment of free radical production demonstrated that THS induced significantly less oxidative stress than conventional CS in osteoprogenitor cells.CONCLUSIONTHS was significantly less harmful to osteoprogenitor cells during osteogenesisthan conventional CS. Additional studies are required to confirm whether THS isa better alternative for smokers to improve delays in bone healing followingfracture. | Romina H Aspera-Werz Sabrina Ehnert Monja Müller Sheng Zhu Tao Chen Weidong Weng Johann Jacoby Andreas K Nussler | 2020 | World Journal of Stem Cells2020，12，8： | 1 |
| 2 | New tacrine- 4-oxo-4H-chromene hybrids as muhifunctional agents for the treatment of Alzheimer's disease, with cholinergic, antioxidant, and beta-amyloid-reducing properties显示文摘 | Perez MI C Monjas L Rademann J | 2012 | J Med Chem2012，55，3： | 1 |
| 3 | Muhipotent, per- meable drug ASS234 inhibits Abeta aggregation, pos- sesses antioxidant properties and protects fi'om Abeta- induced apoptosis in vitro 显示文摘 | Bolea I Gella A Monjas L | 2013 | Curr Alzheimer Res2013，10，8： | 1 |
| 4 | New tacrine-a-oxo- 4H-chromene hybrids as multifunctional agents for the treatment of Alzheimer's disease, with cholinergic, antioxidant, and beta-amyloidreducing properties显示文摘 | Fernandez-Bachiller M I Perez C Monjas L | 2012 | J Med Chern2012，55，3： | 1 |
| 5 | New tacrine-4-oxo- 4H-chromene hybrids as multifunctional agents for the treatment of Alzheimer's disease, with cholinergic, antioxidant, and beta-amyloidreducing properties显示文摘 | Fernandez-Bachiller M I Perez C Monjas L | 2012 | J Med Chern2012，55，3： | 1 |
| 6 | The downregulation of the pro - apoptotic protein Par - 4 is critical for Ras - induced survival and tumor progression显示文摘 | Barradas M Monjas A Diaz- Meco MT | 1999 | EMBO J1999，18，22： | 1 |
| 7 | Inactivation of the inhibitory KB protein kinase/nuclear factor KB pathway by Par -4 expression potentiates tumor necrosis factor α - induced apoptosis 显示文摘 | Diaz- Meco MT Lallena MJ Monjas A | 1999 | J Biol Chem1999，274，19： | 1 |
| 8 | Continuous Arterivenous Rewarming:Experimental Results and Thermodynamic Model Simulation of Treatment for Hypothermia显示文摘 | Gentilello L M Cortes V Monjaes S | 1990 | J Trauma1990，30，12： | 1 |
| 9 | The downregu- lation of the proapoptotic protein Par-4 is critical for Ras- induced survival and tumor progression显示文摘 | Barradas M Monjas Diaz-Meco MT | 1999 | EMBO J1999，18，22： | 1 |
| 10 | TCR engagement induces proline rich tyrosine kinase-2(Pyk2) translocation to the T cell-APC interface independently of Pyk2 activity and in an immunoreceptor tyrosine-based activation motif-mediated fashion显示文摘 | Sancho D Montoya MC Monjas A | 2002 | J Immunol2002，169，1： | 1 |
| 11 | Sound processing in amateur musicians and nonmusicians: event-related potential and behavioral indices显示文摘 | Mari Tervaniemi Anu Castaneda Monja Knoll Maria Uther | 2006 | NeuroReport2006，，11： | 1 |
| 12 | New Tacrine-4-Oxo-4H-chromene hybrids as multifunctional agents for the treatment of Alzheimer's disease,with Cho-linergic,Antioxidant,and β-Amyloid-Reducing properties显示文摘 | Fernández-Bachiller MI Pérez C Monjas L | | 0，，03： | 1 |
| 13 | Insights into the hydrogen evolution reaction in vanadium redox flow batteries:A synchrotron radiation based X-ray imaging study显示文摘The parasitic hydrogen evolution reaction(HER)in the negative half-cell of vanadium redox flow batteries(VRFBs)causes severe efficiency losses.Thus,a deeper understanding of this process and the accompanying bubble formation is crucial.This benchmarking study locally analyzes the bubble distribution in thick,porous electrodes for the first time using deep learning-based image segmentation of synchrotron X-ray micro-tomograms.Each large three-dimensional data set was processed precisely in less than one minute while minimizing human errors and pointing out areas of increased HER activity in VRFBs.The study systematically varies the electrode potential and material,concluding that more negative electrode potentials of-200 m V vs.reversible hydrogen electrode(RHE)and lower cause more substantial bubble formation,resulting in bubble fractions of around 15%–20%in carbon felt electrodes.Contrarily,the bubble fractions stay only around 2%in an electrode combining carbon felt and carbon paper.The detected areas with high HER activity,such as the border subregion with more than 30%bubble fraction in carbon felt electrodes,the cutting edges,and preferential spots in the electrode bulk,are potential-independent and suggest that larger electrodes with a higher bulk-to-border ratio might reduce HER-related performance losses.The described combination of electrochemical measurements,local X-ray microtomography,AI-based segmentation,and 3D morphometric analysis is a powerful and novel approach for local bubble analysis in three-dimensional porous electrodes,providing an essential toolkit for a broad community working on bubble-generating electrochemical systems. | Kerstin Köble Alexey Ershov Kangjun Duan Monja Schilling Alexander Rampf Angelica Cecilia TomášFaragó Marcus Zuber Tilo Baumbach Roswitha Zeis | 2024 | Journal of Energy Chemistry2024，91，4： | 0 |
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