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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 3 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 4 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 5 | A 60 dB 246 MHz CMOS variable gain amplifier for subsampling GSM receivers 显示文摘 | MOSTAFA M A I EMBABI S H K ELMALA M | 2003 | IEEE Transactions on Very Large Scale Integra- tion (VLSI) Systems2003，11，5： | 1 |
| 6 | Folded novel accurate analytical and semi-analytical solutions of a generalized Calogero–Bogoyavlenskii–Schiff equation显示文摘This paper studies the analytical and semi-analytic solutions of the generalized Calogero–Bogoyavlenskii–Schiff(CBS)equation.This model describes the(2+1)–dimensional interaction between Riemann-wave propagation along the y-axis and the x-axis wave.The extended simplest equation(ESE)method is applied to the model,and a variety of novel solitarywave solutions is given.These solitary-wave solutions prove the dynamic behavior of soliton waves in plasma.The accuracy of the obtained solution is verified using a variational iteration(VI)semi-analytical scheme.The analysis and the match between the constructed analytical solution and the semi-analytical solution are sketched using various diagrams to show the accuracy of the solution we obtained.The adopted scheme’s performance shows the effectiveness of the method and its ability to be applied to various nonlinear evolution equations. | Mostafa M A Khater S K Elagan M A El-Shorbagy S H Alfalqi J F Alzaidi Nawal A Alshehri | 2021 | Communications in Theoretical Physics2021，73，9： | 1 |
| 7 | Schiff bases as corrosion inhibitors for aluminium in hydrochloric acid solution 显示文摘 | Gomma Gamal K Wahdan Mostafa H | 1995 | Materials Chemistry and Physics1995，39，3： | 1 |
| 8 | An equation for estimating watersaturation in clean formations utilizing resistivity and soniclogs:Theory and application显示文摘 | Mostafa H K Walid M M | 2002 | Journal of Petroleum Scienceand Engineering2002，36，： | 1 |
| 9 | Prevalence of depression,anxiety and stress as measured by the depression,anxiety,and stress scale(DASS-42)among secondary school girls in Abha,Saudi Arabia显示文摘 | Al-Gelban K S Al-Amri H S Mostafa O A | 2009 | Sultan Qaboos Univ Med J2009，9，2： | 1 |
| 10 | Effect of atrazine herbicide on growth, photosynthesis, protein synthesis and fatty acid composition in the unicellular alga Chlorella kessleri 显示文摘 | Mostafa M E Hossam M K and Ola H E | 1994 | Ecotoxic Environ Safe1994，29，： | 1 |
| 11 | Relationship Between Housing Affordability and Economic Development in Main- land China - case of Shanghai 显示文摘 | MOSTAFA A WONG F K CHI M H | 2006 | Journal of Urban Plan- ning and Development2006，132，1： | 1 |
| 12 | A 60 - dB 246 - MHz CMOS variable gain amplifier for sub - sampiing GSM receivers 显示文摘 | Mohamed A I Mostafa Sherif H K Elmala M | 2003 | IEEE Transactions on Very Large Scale Integration (VLSI) System2003，11，5： | 1 |
| 13 | Porosity estimation using a combination of Wyllie-Clemenceau equations in clean sand formation from acoustic logs显示文摘 | Mostafa H K Walid M M Abdelrahim I | 2002 | Journal of Petroleum Science and Engineering2002，33，： | 1 |
| 14 | Positional effect of mutations in 5'UTR of hepatitis C virus 4a on patients' response to therapy显示文摘AIM:To investigate the effects of mutations in domain Ⅲ of the hepatitis C virus(HCV)internal ribosome entry sequences(IRES)on the response of chronic HCV genotype 4a patients to interferon therapy.METHODS:HCV RNA was extracted from 19 chronic HCV 4a patients receiving interferon/ribavirin therapy who showed dramatic differences in their response to combination therapy after initial viral clearance.IRES domainⅢ was cloned and 15 clones for each patient were sequenced.The obtained sequences were aligned with genotype 4a prototype using the ClustalW program and mutations scored.Prediction of stem-loop secondary structure and thermodynamic stability of the major quasispecies in each patient was performed using the MFOLD 3.2 program with Turner energies and selected constraints on base pairing.RESULTS:Analysis of RNA secondary structure revealed that insertions in domainⅢ altered WatsonCrick base pairing of stems and reduced molecular stability of RNA,which may ultimately reduce binding affinity to ribosomal proteins.Insertion mutations in domainⅢwere statistically more prevalent in sustained viral response patients(SVR,n=14)as compared to breakthrough(BT,n=5)patients.CONCLUSION:The influence of mutations within domainⅢ on the response of HCV patients to combination therapy depends primarily on the position,but not the frequency,of these mutations within IRES domain Ⅲ. | Mostafa K El Awady Hassan M Azzazy Ahmed M Fahmy Sherif M Shawky Noha G Badreldin Samar S Yossef Moataza H Omran Abdel Rahman N Zekri Said A Goueli | 2009 | World Journal of Gastroenterology2009，15，12： | 1 |
| 15 | Porous lanthanide-organic framework with zeolite-like topology显示文摘 | Maji T K Mostafa G Changa H C | 2005 | Chem Comm2005，，： | 1 |
| 16 | Antibody to El peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro显示文摘瞄准:对丙肝病毒(HCV ) 的 E1 区域分析抗体的抵销的活动。特定的 polyclonal 抗体与从 HCV 的 E1 区域被导出并且被显示高度在 HCV 之中被保存的合成的肽经由新西兰兔子的免疫被提起出版遗传型。方法:超 HCV E1 抗体与为 HCV RNA 积极的浆液样品在 4 度摄氏在夜里被孵化,与从 615 ~ 3.2 百万 IU/ mL 的病毒的负担。对待的重量的单位一为 90 min 与 HepG2 房间被孵化。由反 E1 抗体病毒的绑定和入口堵住进房间借助于 RT-PCR 和流动血细胞计数被测试。结果:用 FITC 染色的直接免疫结合了分析显示出的流动 cytometric 跟随的 E1 抗体在样品的减少的吝啬的荧光紧张与未经治疗的样品相比与 E1 抗体预先孵化。而且, 13 从 18 积极重量的单位一(72%) 显示出由 RT-PCR 检测了的传染性的完全的抑制。结论:在房子里生产 E1 抗体,块绑定和到在病毒绑定建议这 epitope 的参与的靶细胞的 HCV virion 感染的入口和入口。堵住病毒附件到人的房间的这些抗体的隔离作为治疗学的试剂有用。 | Mostafa K EL-Awady Ashraf A Tabll Khaled Atef Samar S Yousef Moataza H Omran Yasmin EI-Abd Noha G Bader-Eldin Ahmad M Salem Samir F Zohny Wael T EI-Garf | 2006 | World Journal of Gastroenterology2006，12，16： | 0 |
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