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| 1 | Preparation, character- ization, and supercritical carbon dioxide foaming of poly- styrene/graphene oxide composites 显示文摘 | Yang Jintao Mu Minjie | 2011 | The Journal of Supercritical Fluids2011，56，2： | 1 |
| 2 | Purification and identification of antioxidant peptides from sweet potato protein hydrolysates by Alcalase显示文摘 | ZHANG Miao MU Taihua SUN Minjie | 2014 | Journal of Functional Foods2014，7，： | 1 |
| 3 | Purification and identification of antioxidant peptides from sweet potato pro- tein hydrolysates by Alcalase显示文摘 | Zhang Miao Mu Taihua Sun Minjie | 2014 | Journal of Functional Foods2014，7，： | 1 |
| 4 | Purification and identification of antioxidant peptides from sweet potato protein hydrolysates by Alcalase显示文摘 | Zhang Miao Mu Taihua Sun Minjie | 2014 | Journal of Functional Food2014，7，： | 1 |
| 5 | Characterization of two chimeric sesterterpene synthases from a fungal symbiont isolated from a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum显示文摘Two chimeric sesterterpene synthases(Aa TPS1 and Aa TPS2)were functionally characterized from Alternaria alternata MB-30 isolated from the leaves of a sesterterpenoid-producing Lamiaceae plant Leucosceptrum canum.Aa TPS1 generated a 5/8/6/5 tetracyclic sesteraltererol(1)and its absolute stereochemistry was determined by X-ray crystallographic analysis of its derivative 10,11-epoxysesteraltererol(2),which enabled revision of the absolute configuration of C7 of sesterfisherol produced by Nf SS and PTTS014 characterized previously and its derivative 10,11-epoxysesterfisherol.Aa TPS2 produced a 5/15 bicyclic preterpestacin I(3).Site-directed mutagenesis suggested that F192 in Aa TPS1 was likely involved in controlling of the hydroxylation of C12,and eight amino acids were important for the enzyme activity of Aa TPS1 and Aa TPS2.The engineered Escherichia coli and Saccharomyces cerevisiae strains were constructed for the productions of compounds 1 and 3,and the highest titer of compound 1 reached 62.3 mg/L in shake-flask culture.Both compounds 1 and 2 showed anti-adipogenic activity. | Desen Li Minjie Yang Rongfang Mu Shihong Luo Yuegui Chen Wenyuan Li An Wang Kai Guo Yan Liu Shenghong Li | 2023 | Chinese Chemical Letters2023，34，1： | 1 |
| 6 | Unraveling the serial glycosylation in the biosynthesis of steroidal saponins in the medicinal plant Paris polyphylla and their antifungal action显示文摘Sugar-sugar glycosyltransferases play important roles in constructing complex and bioactive saponins.Here,we characterized a series of UDP-glycosyltransferases responsible for biosynthesizing the branched sugar chain of bioactive steroidal saponins from a widely known medicinal plant Paris polyphylla var.yunnanensis.Among them,a 2'-O-rhamnosyltransferase and three 6'-O-glucosyltrasferases catalyzed a cascade of glycosylation to produce steroidal diglycosides and triglycosides,respectively.These UDP-glycosyltransferases showed astonishing substrate promiscuity,resulting in the generation of a panel of 24 terpenoid glycosides including 15 previously undescribed compounds.A mutant library containing 44 variants was constructed based on the identification of critical residues by molecular docking simulations and protein model alignments,and a mutant UGT91AH1^(Y187A)with increased catalytic efficiency was obtained.The steroidal saponins exhibited remarkable antifungal activity against four widespread strains of human pathogenic fungi attributed to ergosterol-dependent damage of fungal cell membranes,and 2'-O-rhamnosylation appeared to correlate with strong antifungal effects.The findings elucidated the biosynthetic machinery for their production of steroidal saponins and revealed their potential as new antifungal agents. | Yuegui Chen Qin Yan Yunheng Ji Xue Bai Desen Li Rongfang Mu Kai Guo Minjie Yang Yang Tao Jonathan Gershenzon Yan Liu Shenghong Li | 2023 | Acta Pharmaceutica Sinica B2023，13，11： | 0 |
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