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| 1 | RNA interference-mediated silencing of vATPase subunits A and E affect survival and development of the 28-spotted ladybeetle,Henosepilachna vigintioctopunctata显示文摘RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia.In this study,the potential of dietary RNAi to manage H.vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double-stranded RNAs(dsRNAs)of HvvATPase A and HvvATPase E.The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types.The silencing of HvvATPase A and HvvATPase E led to significant mortality in H.vigintioctopunctata larvae.In addition,the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H.vigintioctopunctata.Notably,the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults.Finally,the nontarget effects of the dsRNAs of H.vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated.P.japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H.vigintioctopunctata and P.japonica under the worst-case scenario,in which dsGFP served as negative control.There were significant effects of dsHvvATPase A on P.japonica at the transcriptional level but not at the organismal level,whereas dsHvvATPase E did not effect P.japonica at either the transcriptional or the organismal level.Collectively,the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H.vigintioctopunctata. | Wei Guo Mujuan Guo Chunxiao Yang Zhuoqi Liu Shimin Chen Jing Lu Baoli Qiu Youjun Zhang Xuguo Zhou Huipeng Pan | 2021 | Insect Science2021，28，6： | 3 |
| 2 | 3D preform design in forging process based on quasi-quipotential field and response surface methods显示文摘 | GUAN Yanjin BAI Xue LIU Mujuan | 2014 | Procedia Engineering2014，81，： | 1 |
| 3 | 3D Preform Design in Forging Process based on Quasi-quipotential Field and Response Surface Methods显示文摘 | Yanjin Guan Xue Bai Mujuan Liu Libin Song Guoqun Zhao | 2014 | Procedia Engineering2014，，： | 1 |
| 4 | Selenized liposomes with ameliorative stability that achieve sustained release of emodin but fail in bioavailability显示文摘Stability of liposomes plays a crucial role in drug delivery,especially in oral aspect.The structural modification of liposomes has been the orientation of efforts to improve their stability and enable the controllability of payload release.This study reported a selenylation strategy to optimize the liposomal structure in an attempt to enhance the nanocarrier’s stability,hence the bioavailability of emodin(EM),an active compound with poor water-solubility.EM-loaded selenized liposomes(EM-Se@LPs)were prepared by thin film dispersion followed by in situ reduction technique.The results showed that EM-Se@LPs were provided with enhancive gastrointestinal stability and exhibited sustained release of drug compared with EM-loaded liposomes(EM-LPs).However,the modified liposomes with Se depositing onto the interior and exterior bilayers did not substantially facilitate absorption of EM.The reinforced structure of liposomes irrelevant to absorption was affirmed to be due to good stability and absorbability of EM itself.Nevertheless,the present work provides an alternative option for stabilization of liposomes instead of conventional methods,which may be promising for oral delivery of physiologically unstable and/or poorly absorbed drugs and systemic drug delivery. | Mujuan Zhu Shiping Zhu Qiubo Liu Yuehong Ren Zhiguo Ma Xingwang Zhang | 2023 | Chinese Chemical Letters2023，34，1： | 0 |
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