| 2 | Human papillomavirus in upper digestive tract tumors from three countries显示文摘AIM: To clarify human papillomavirus (HPV) involvement in carcinogenesis of the upper digestive tract of virological and pathological analyses. METHODS: The present study examined the presence of HPV in squamous cell carcinomas of the oral cavity (n = 71), and esophagus (n = 166) collected from Japan, Pakistan and Colombia, with different HPV exposure risk and genetic backgrounds. The viral load and physical status of HPV16 and HPV16-E6 variants were examined. Comparison of p53 and p16INK4a expression in HPV-positive and HPV-negative cases was also made. RESULTS: HPV16 was found in 39 (55%) oral carcinomas (OCs) and 24 (14%) esophageal carcinomas (ECs). This site-specific difference in HPV detection between OCs and ECs was statistically significant (P < 0.001). There was a significant difference in the geographical distribution of HPV16-E6 variants. Multiple infections of different HPV types were found in 13 ECs, but multiple infections were not found in OCs. This difference was statistically significant (P = 0.001). The geometric means (95% confidence interval) of HPV16 viral load in OCs and ECs were 0.06 (0.02-0.18) and 0.12 (0.05-0.27) copies per cell, respectively. The expression of p16INK4a proteins was increased by the presence of HPV in ECs (53% and 33% in HPV-positive and-negative ECs, respectively; P = 0.036), and the high-risk type of the HPV genome was not detected in surrounding normal esophageal mucosa of HPV-positive ECs. CONCLUSION: Based on our results, we cannot deny the possibility of HPV16 involvement in the carcinogenesis of the esophagus. | Andres Castillo Chihaya Koriyama Michiyo Higashi Muhammad Anwar Mulazim Hussain Bukhari Edwin Carrascal Lida Mancilla Hiroshi Okumura Masataka Matsumoto Kazumasa Sugihara Shoji Natsugoe Yoshito Eizuru Suminori Akiba | 2011 | World Journal of Gastroenterology2011，17，48： | 1 |
| 4 | Frequency of HER2/neu overexpression in adenocarcinoma of the gastrointestinal system显示文摘AIM:To determine the frequency of HER2/neu protein overexpression in gastric(group A),small intestine(group B),and colorectal(group C)adenocarcinoma.METHODS:A descriptive,cross-sectional study was performed on 50 cases of gastrointestinal adenocarcinoma(stomach,small intestine,and colorectal);11from group A,8 from group B,and 31 from group C.The samples were grossed and processed in the pathology department,and sections were stained with HE(hematoxylin and eosin stain)for histopathological confirmation of malignancy(well-differentiated,moderately-differentiated,and poorly-differentiated).The confirmed samples were processed for immunomarker study of HER2/neu.RESULTS:HER2/neu protein overexpression was found in 33(66%)patients overall(P=0.000).Out of 33HER2/neu positive subjects,23(69.6%)were from group C,while the remaining 10(30%)were from group A.None of the patients from group B had positive HER2/neu protein overexpression.No protein overexpression or membrane staining in<10%tumor cells was observed in 17(34%)patients,which were labeled as score'0'and considered negative for HER2/neu protein overexpression.Faint/weak staining(in≥10%of tumors cells)were observed in 8(16%)patients and given the'1+'score.Similarly 13(26%)patients reported moderate staining(in≥10%tumor cells)and were thus labeled as'2+',and strong staining(in≥10%tumors cells),labeled as'3+',was observed in 12(24%)patients.Out of 50 patients,26(52%)were suffering from grade-Ⅱmalignancy,16(32%)from grade-Ⅰ,and 8(16%)from grade-Ⅲ.There was highly significant association between tumor grades and HER2/neu protein overexpression(P=0.0000).CONCLUSION:HER2/neu protein is credibly overexpressed in colon and gastric adenocarcinomas in immunohistochemistry.There is significant association between grade of tumor and HER2/neu protein overexpression. | Sadaf Farzand Tahir Siddique Kanwal Saba Mulazim Hussain Bukhari | 2014 | World Journal of Gastroenterology2014，20，19： | 0 |
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