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| 1 | Natural populations of lactic acid bacteria in douchi from Yunnan Province,China显示文摘This research was aimed at isolating and identifying the predominant lactic acid bacteria(LAB) in the traditional Chinese salt-fermented soybean food,douchi,from Yunnan,China.The predominant LAB present were isolated and identified by conventional culture-dependent methods combined with molecular biological methods.Two hundred and sixty isolates were obtained from thirty kinds of traditional fermented douchi from six cities and counties in Yunnan,and those strains were divided into twelve groups by their morphological and biochemical characteristics.Based on 16S ribosomal DNA(rDNA) sequencing and analysis,56 representative strains were identified as belonging to 6 genera and 14 species:Lactobacillus(4 spp.),Weissella(3 spp.),Pediococcus(2 spp.),Staphylococcus(2 spp.),Enterococcus(1 sp.),and Bacillus(2 spp.).The results show that douchi contains a large natural population of LAB of diverse composition from which some strains could be selected as starters for functional fermented foods.This is the first study on the original douchi from Yunnan,and the results suggest that it may be a useful source for the isolation of LAB.This study has also laid a foundation for further research on developing functional douchi products. | Chen-jian LIU Fu-ming GONG Xiao-ran LI Hai-yan LI Zhong-hua ZHANG Yue FENG Hiroko NAGANO | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，4： | 16 |
| 2 | 两个华支睾吸虫半胱氨酸蛋白酶的基因克隆、表达与定性(英文)显示文摘目的 寻找对华支睾吸虫病诊断有效的抗原基因,以便生物合成并研究其应用价值。方法 选取两个Genbank已报告的基因序列AF271091(CysA)及AF093242(CysB)分别克隆与表达融合蛋白,亲和层析纯化后进行免疫定性。结果 CysA与CysB均获得成功克隆与表达,Western blot分析显示CysA与华支睾吸虫病阳性血清有强反应,与日本血吸虫病阳性血清只有很弱的交叉反应,而CysA与华支睾吸虫感染血清无反应。免疫组化显示,被表达的两个半胱氨酸蛋白酶在华支睾吸虫体内的免疫定位无显著不同,主要在成虫肠道及虫卵。结论 成功表达的两个半胱氨酸蛋白酶中,CysB针对华支睾吸虫病血清有良好的抗原活性,值得视为诊断抗原候选做进一步的研究。 | 裴福全 Isao Nagano 吴军 WuZhi-liang 崔惠儿 Yuzo Takahashi 潘波 方悦怡 | 2004 | 热带医学杂志2004，4，1： | 9 |
| 3 | Advantage of autologous blood transfusion in surgery for hepatocellular carcinoma显示文摘AIM: To evaluate the significance of autologous blood transfusion (AT) in reducing homologous blood transfusion (HT) in surgery for hepatocellular carcinoma (HCC). METHODS: The proportion of patients who received HT was compared between two groups determined by the time of AT introduction; period A (1991-1994, n = 93) and period B (1995-2000, n = 201). Multivariate logistic regression analysis was performed in order to identify independent significant predictors of the need for HT. We also investigated the impact of AT and HT on long-term postoperative outcome after curative surgery for HCC. RESULTS: The proportion of patients with HT was significantly lower in period B than period A (18.9% vs 60.2%, P < 0.0001). Multivariate logistic regression analysis identified AT administration as a significant independent predictor of the need for HT (P < 0.0001). Disease-free survival in patients with AT was comparable to that without any transfusion. Multivariate analysis identified HT administration as an independent significant factor for poorer disease-free survival (P = 0.0380). CONCLUSION: AT administration significantly decreased the need for HT. Considering the postoperative survival disadvantage of HT, AT administration could improve the long-term outcome of HCC patients. | Yoshito Tomimaru Hidetoshi Eguchi Shigeru Marubashi Hiroshi Wada Shogo Kobayashi Masahiro Tanemura Koji Umeshita Yuichiro Doki Masaki Mori Hiroaki Nagano | 2011 | World Journal of Gastroenterology2011，17，32： | 8 |
| 4 | CD13 is a therapeutic target in human liver cancer stem cells显示文摘 | Haraguchi Naotsugu Ishii Hideshi Mimori Koshi Tanaka Fumiaki Ohkuma Masahisa Kim Ho Min Akita Hirofumi Takiuchi Daisuke Hatano Hisanori Nagano Hiroaki Barnard Graham F Doki Yuichiro Mori Masaki | 2010 | Journal of Clinical Investigation2010，，9： | 7 |
| 5 | 华支睾吸虫半胱氨酸蛋白酶的基因表达与在ELISA诊断上的应用研究(英文)显示文摘目的探讨重组华支睾吸虫半胱氨酸蛋白酶在华支睾吸虫病血清学诊断上的应用价值。方法以成虫cDNA为模板PCR扩增GenBank中一个华支睾吸虫半胱氨酸蛋白酶(No.AF093242,简称CysB)的部分基因片段,亚克隆到pTrcHis表达载体,转化入大肠埃希菌DH5α。表达出的重组蛋白经亲合层析纯化后用于ELISA检测华支睾吸虫及其他寄生虫感染血清,评价其诊断应用价值。结果成功克隆与表达CysB基因片段,纯化后的重组蛋白用于华支睾吸虫病诊断的敏感性达96.0%(48/50),与其他寄生虫病的总交叉反应为3.8%(1/26),而对比实验中可溶性粗抗原(CAA)用于检测的敏感性为88.0%(44/50),与其他寄生虫感染血清无交叉反应。结论重组华支睾吸虫半胱氨酸蛋白酶(CysB)用于ELISA诊断技术具有较高的敏感性及特异性,有希望成为可溶性粗抗原的替代之一。 | 裴福全 Isao Nagano Zhiliang Wu 吴军 阮彩文 崔惠儿 Yuzo Takahashi 方悦怡 | 2005 | 中国寄生虫病防治杂志2005，18，2： | 6 |
| 6 | Chronic hepatitis C virus infection and lipoprotein metabolism显示文摘Hepatitis C virus(HCV) is a hepatotrophic virus and a major cause of chronic liver disease,including hepatocellular carcinoma,worldwide. The life cycle of HCV is closely associated with the metabolism of lipids and lipoproteins. The main function of lipoproteins is transporting lipids throughout the body. Triglycerides,free cholesterol,cholesteryl esters,and phospholipids are the major components of the transported lipids. The pathway of HCV assembly and secretion is closely linked to lipoprotein production and secretion,and the infectivity of HCV particles largely depends on the interaction of lipoproteins. Moreover,HCV entry into hepatocytes is strongly influenced by lipoproteins. The key lipoprotein molecules mediating these interactions are apolipoproteins. Apolipoproteins are amphipathic proteins on the surface of a lipoprotein particle,which help stabilize lipoprotein structure. They perform a key role in lipoprotein metabolism by serving as receptor ligands,enzyme co-factors,and lipid transport carriers. Understanding the association between the life cycle of HCV and lipoprotein metabolism is important because each step of the life cycle of HCV that is associated with lipoprotein metabolism is a potential target for anti-HCV therapy. In this article,we first concisely review the nature of lipoprotein and its metabolism to better understand the complicated interaction of HCV with lipoprotein. Then,we review the outline of the processes of HCV assembly,secretion,and entry into hepatocytes,focusing on the association with lipoproteins. Finally,we discuss the clinical aspects of disturbed lipid/lipoprotein metabolism and the significance of dyslipoproteinemia in chronic HCV infection with regard to abnormal apolipoproteins. | Yoshio Aizawa Nobuyoshi Seki Tomohisa Nagano Hiroshi Abe | 2015 | World Journal of Gastroenterology2015，21，36： | 6 |
| 7 | Sclerosing epithelioid fibrosarcoma of the liver infiltrating the inferior vena cava显示文摘Sclerosing epithelioid fibrosarcoma (SEF) is a rare and distinct variant of fibrosarcoma, composed of epithelioid tumor cells arranged in strands, nests, cords, or sheets embedded within a sclerotic collagenous matrix. We report a 39-year-old man with SEF of the liver, which infiltrated the inferior vena cava (IVC). The SEF of the liver was successfully resected, and the infiltrated IVC was also removed together with the liver tumor. Histopathological examination of the tumor showed typical histopathology of SEF. Immunohistochemically, the tumor was positive for vimentin. Recurrence was noted 7 mo after surgery. After chemotherapy, the recurrent tumor was resected surgically, and histopathological examination showed similar findingsto those of the primary tumor. To our knowledge, this is the first report of SEF of the liver with tumor invasion of the IVC. | Yoshito Tomimaru Hiroaki Nagano Shigeru Marubashi Shogo Kobayashi Hidetoshi Eguchi Yutaka Takeda Masahiro Tanemura Toru Kitagawa Koji Umeshita Nobuyuki Hashimoto Hideki Yoshikawa Kenichi Wakasa Yuichiro Doki Masaki Mori | 2009 | World Journal of Gastroenterology2009，15，33： | 5 |
| 8 | Fresh frozen plasma transfusion does not affect outcomes following hepatic resection for hepatocellular carcinoma显示文摘AIM:To investigate whether fresh frozen plasma (FFP) transfusion affects outcomes following hepatic resection for hepatocellular carcinoma (HCC) in terms of liver function,postoperative complications and cancer prognosis.METHODS:We retrospectively compared the incidence of postoperative complications between 204 patients who underwent hepatectomy for HCC with routine FFP transfusion in an early period (1983-1993,Group A) and 293 with necessity for FFP transfusion during a later period (1998-2006,Group B),and also between two subgroups of Group B [22 patients with FFP transfusion (Group B1) and 275 patients without FFP transfusion (Group B2)].Additionally,only in limited patients in Group B1 and Group B2 with intraoperative blood loss≥ 2000 mL (Group B1 ≥ 2000 mL and Group B2 ≥ 2000 mL),postoperative complications,liver function tests,and cancer prognosis were compared.RESULTS:No mortality was registered in Group B,compared to 8 patients (3.9%) of Group A.The incidence of morbidity in Group B2 [23.2% (64/275)] was not significantly different from Group B1 [40.9% (9/22)] and Group A [27.0% (55/204)].The incidence of complications and postoperative liver function tests were comparable between Group B1 ≥ 2000 mL vs Group B2 ≥ 2000 mL.Postoperative prognosis did not correlate with administration of FFP,but with tumor-related factors.CONCLUSION:The outcome of hepatectomy for HCC is not influenced by FFP transfusion.We suggest FFP transfusion be abandoned in patients who undergo hepatectomy for HCC. | Yoshito Tomimaru Hiroshi Wada Shigeru Marubashi Shogo Kobayashi Hidetoshi Eguchi Yutaka Takeda Masahiro Tanemura Takehiro Noda Koji Umeshita Yuichiro Doki Masaki Mori Hiroak Nagano | 2010 | World Journal of Gastroenterology2010，16，44： | 5 |
| 9 | Anticancer effect of linalool via cancer-specific hydroxyl radical generation in human colon cancer显示文摘AIM To investigate the anticancer mechanisms of the monoterpenoid alcohol linalool in human colon cancer cells.METHODS The cytotoxic effect of linalool on the human colon cancer cell lines and a human fibroblast cell line was examined using the WST-8 assay. The apoptosisinducing effect of linalool was measured using the terminal deoxynucleotidyl transferase d UTP nickend labeling assay and flow cytometry with Annexin V. Oxidative stress was investigated by staining for diphenyl-1-pyrenylphosphine, which is a cellular lipid peroxidation marker, and electron spin resonance spectroscopy. Sixteen SCID mice xenografted with human cancer cells were randomized into 3 groups for in vivo analysis: control and low-dose and high-dose linalool groups. The control group was administered tap water orally every 3 d. The linalool treatment groups were administered 100 or 200 μg/kg linalool solution orally for the same period. All mice were sacrificed under anesthesia 21 d after tumor inoculation, and tumors and organs were collected for immunohistochemistry using an anti-4-hydroxynonenal antibody. Tumor weights were measured and compared between groups. RESULTS Linalool induced apoptosis of cancer cells in vitro, following the cancer-specific induction of oxidative stress, which was measured based on spontaneous hydroxyl radical production and delayed lipid peroxidation. Mice in the high-dose linalool group exhibited a 55% reduction in mean xenograft tumor weight compared with mice in the control group(P < 0.05). In addition, tumor-specific lipid peroxidation was observed in the in vivo model.CONCLUSION Linalool exhibited an anticancer effect via cancerspecific oxidative stress, and this agent has potential for application in colon cancer therapy. | Kenichi Iwasaki Yun-Wen Zheng Soichiro Murata Hiromu Ito Ken Nakayama Tomohiro Kurokawa Naoki Sano Takeshi Nowatari Myra O Villareal Yumiko N Nagano Hiroko Isoda Hirofumi Matsui Nobuhiro Ohkohchi | 2016 | World Journal of Gastroenterology2016，22，44： | 5 |
| 10 | Bile duct hamartomas(von Mayenburg complexes) mimicking liver metastases from bile duct cancer:MRC findings显示文摘我们在场是的有胆总管癌症的一个 72 岁的人的一个盒子开始相信了吃多重肝转移基于计算断层摄影术调查结果,并且在磁性的回声胆管造影术(MRC ) 在谁揭示胆汁管 hamartomas 的诊断。在为 pancreaticoduodenectomy 的探索,肝触诊在肝的表面揭示了传播小瘤。这些小瘤在在两片肝脑叶的表面上散布的直径显示出大约 0.5 厘米的灰色白人的榴状的损害,它被看起来像从胆汁管癌的多重肝转移。肝活体检视的冰冻切片揭示了多重胆汁管,稍微扩大的腔在多重胆汁管 hamartomas (BDH ) 的成胶的基质特征嵌入。仅仅二份报告描述了胆汁管 hamartomas 的 MRC 特征。所有成像过程, MRC 为胆汁管 hamartomas 的成像诊断提供最相关的特征。 | Yasuhiko Nagano Kenichi Matsuo Katsuya Gorai Kazuya Sugimori Chikara Kunisaki Hideyuki Ike Katsuaki Tanaka Toshio Imada Hiroshi Shimada | 2006 | World Journal of Gastroenterology2006，12，8： | 4 |
| 11 | Serum Mac-2 binding protein is a novel biomarker for chronic pancreatitis显示文摘AIM: To determine the efficacy of Mac-2 binding protein(Mac-2bp) for diagnosis of chronic pancreatitis.METHODS: Fifty-nine healthy volunteers(HV), 162 patients with chronic pancreatitis(CP), and 94 patients with pancreatic ductal adenocarcinoma(PDAC) were enrolled in this study. We measured serum Mac-2bp using our developed enzyme-linked immunosorbent assay kit. Additional biochemical variables were measured using an automated analyzer(including aminotransferase, alanine aminotransferase, γ-glutamyltransferase, alkaline phosphatase, triglyceride, C-reactive protein, and amylase levels) or chemiluminescent enzyme immunoassay(carbohydrate antigen 19-9 and carcinoembryonic antigen). The ability of Mac-2bp to predict CP diagnosis accurately was assessed using receiver operating characteristic(ROC) analyses.RESULTS: Serum Mac-2bp levels were significantly increased in CP patients compared to HV(P < 0.0001) and PDAC patients(P < 0.0001). Area under the ROC curve values of Mac-2bp for the discrimination of CP from HV and PDAC were 0.727 and 0.784, respectively. Multivariate analyses demonstrated that serum Mac-2bp levels were independent determinants for CP diagnosis from HV and PDAC patients. Immunohistological staining showed that Mac-2bp was expressed faintly in the pancreas tissues of both CP and PDAC patients. Serum aspartate aminotransferase, alanine aminotransferase, γ-glutamyltransferase, alkaline phosphatase, and triglyceride levels were significantly higher in patients with CP or PDAC. Serum Mac-2bp levels were highly correlated with protein levels of alanine aminotransferase, γ-glutamyltransferase, and C-reactive protein, but not amylase, suggesting that the damaged liver produces Mac-2bp. CONCLUSION: Measurement of serum Mac-2bp may be a novel and useful biomarker for CP diagnosis as well as liver fibrosis in the general population. | Tomohiro Maekawa Yoshihiro Kamada Yusuke Ebisutani Makiko Ueda Tomoki Hata Koichi Kawamoto Shinji Takamatsu Kayo Mizutani Mayuka Shimomura Tomoaki Sobajima Hironobu Fujii Kotarosumitomo Nakayama Kimihiro Nishino Makoto Yamada Takashi Kumada Toshifumi Ito Hidetoshi Eguchi Hiroaki Nagano Eiji Miyoshi | 2016 | World Journal of Gastroenterology2016，22，17： | 3 |
| 12 | Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma显示文摘 | Yoshito Tomimaru Hidetoshi Eguchi Hiroaki Nagano Hiroshi Wada Shogo Kobayashi Shigeru Marubashi Masahiro Tanemura Akira Tomokuni Ichiro Takemasa Koji Umeshita Tatsuya Kanto Yuichiro Doki Masaki Mori | 2011 | Journal of Hepatology2011，，1： | 3 |
| 13 | Hepatic Artery Reconstruction in Living Donor Liver Transplantation: Risk Factor Analysis of Complication and a Role of MDCT Scan for Detecting Anastomotic Stricture显示文摘 | Shigeru Marubashi Shogo Kobayashi Hiroshi Wada Koichi Kawamoto Hidetoshi Eguchi Yuichiro Doki Masaki Mori Hiroaki Nagano | 2013 | World Journal of Surgery2013，，11： | 3 |
| 14 | Equivalent Outcomes After Anatomical and Non-anatomical Resection of Small Hepatocellular Carcinoma in Patients with Preserved Liver Function显示文摘 | Yoshito Tomimaru Hidetoshi Eguchi Shigeru Marubashi Hiroshi Wada Shogo Kobayashi Masahiro Tanemura Koji Umeshita Yuichiro Doki Masaki Mori Hiroaki Nagano | 2012 | Digestive Diseases and Sciences2012，，7： | 2 |
| 15 | A subpopulation of endothelial progenitor cells with low aldehyde dehydrogenase activity attenuates acute ischemic brain injury in rats显示文摘 | Kazuhiro Nakamura Hideo Tsurushima Aiki Marushima Masumi Nagano Toshiharu Yamashita Kensuke Suzuki Osamu Ohneda Akira Matsumura | 2012 | Biochemical and Biophysical Research Communications2012，，1： | 2 |
| 16 | Minute gastric carcinoid tumor with regional lymph node metastasis显示文摘 | Toshihiko Shinohara Shigekazu Ohyama Hideki Nagano Nozomi Amaoka Keiichiro Ohta Toshiki Matsubara Toshiharu Yamaguchi Akio Yanagisawa Yo Kato Tetsuichiro Muto | 2003 | Gastric Cancer2003，，4： | 2 |
| 17 | Differences of bone bonding ability and degradation behaviour in vivo between amorphous calcium phosphate and highly crystalline hydroxyapatite coating显示文摘 | Masahisa Nagano Takashi Nakamura Tadashi Kokubo Masami Tanahashi Masaki Ogawa | 1996 | Biomaterials1996，，18： | 2 |
| 18 | Risk Factors and Management of Bile Leakage after Hepatic Resection显示文摘 | Yasuhiko Nagano M.D. Ph.D. Shinji Togo M.D. Ph.D. Kuniya Tanaka M.D. Ph.D. Hidenori Masui M.D. Ph.D. Itaru Endo M.D. Ph.D. Hitoshi Sekido M.D. Ph.D. Kaoru Nagahori M.D. Ph.D. Hiroshi Shimada M.D. Ph.D | 2003 | World Journal of Surgery2003，，6： | 2 |
| 19 | Different Effects of Absorption Promoters on Corneal and Conjunctival Penetration of Ophthalmic Beta-Blockers显示文摘 | Hitoshi Sasaki Yoshiaki Igarashi Toshiaki Nagano Koyo Nishida Junzo Nakamura | 1995 | Pharmaceutical Research1995，，8： | 2 |
| 20 | 聚酰亚胺的新动向:技术与市场显示文摘近年来,聚酰亚胺的市场有明显的增长。这主要依赖于挠性印刷电路板(FPC)的市场。最近,对精细电路图形FPC的需求增加,使聚酰亚胺薄膜的尺寸稳定性变得非常重要。本文讨论了在FPC制造过程中尺寸变化的机理。近几年,有一种新的动向:热塑性聚酰亚胺的应用增加。本文将介绍一种热塑性聚酰亚胺 KANEKA PIXEO[TM]。 | Hirosaku Nagano Hiroyuki Furutani 蒋东云 | 1999 | 绝缘材料通讯1999，，4： | 2 |
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