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| 1 | Alleviated mucosal and neuronal damage in a rat model of Crohn's disease显示文摘AIM:To establish a rat model suitable to investigate the repetitive relapsing inflammations(RRI)characteristic to Crohn’s disease.METHODS:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were mimicked by repeating administrations of TNBS.Tissue samples were taken from control,once,twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation.The means of the ulcerated area were measured to evaluate the macroscopic mu-cosal damage.The density of myenteric neurons was determined on whole mounts by Hu C/Hu D immunohistochemistry.Heme oxygenase-1(HO-1)expression was evaluated by molecular biological techniques.RESULTS:TNBS-treated rats displayed severe colitis,but the mortality was negligible,and an increase of body weight was characteristic throughout the experimental period.The widespread loss of myenteric neurons,and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration.After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased,and the neuronal loss was on a smaller scale and was limited to the inflamed area.HO-1 m RNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined.Nevertheless,the HO-1protein up-regulation after the second TNBS treatment proved to be transient.Following the third treatment HO-1 protein expression could not be detected.CONCLUSION:Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage.The persistent up-regulation of HO-1 m RNA expression may correlate with this. | Petra Talapka Lajos István Nagy Alexandra Pál Marietta Zita Poles Anikó Berkó Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2014 | World Journal of Gastroenterology2014，20，44： | 3 |
| 2 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 3 | Frequency and prognostic role of mucosal healing in patients with Crohn's disease and ulcerative colitis after one-year of biological therapy显示文摘AIM:To assess the endoscopic activity before and after a one-year period of biological therapy and to evaluate the frequency of relapses and need for retreatment after stopping the biologicals in patients with Crohn’s disease(CD)and ulcerative colitis(UC).METHODS:The data from 41 patients with CD and 22 patients with UC were assessed.Twenty-four CD patients received infliximab,and 17 received adalimumab.The endoscopic severity of CD was quantified with the simplified endoscopic activity score for Crohn’s disease in CD and with the Mayo endoscopic subscore in UC.RESULTS:Mucosal healing was achieved in 23 CD and7 UC patients.Biological therapy had to be restarted in78%of patients achieving complete mucosal healing with CD and in 100%of patients with UC.Neither clinical remission nor mucosal healing was associated with the time to restarting the biological therapy in either CD or UC.CONCLUSION:Mucosal healing did not predict sustained clinical remission in patients in whom the biological therapies had been stopped. | Klaudia Farkas Péter László Lakatos Mónika Szcs va Pallagi-Kunstár Anita Bálint Ferenc Nagy Zoltán Szepes Noémi Vass Lajos S Kiss Tibor Wittmann Tamás Molnár | 2014 | World Journal of Gastroenterology2014，20，11： | 2 |
| 4 | A Short Amino-Terminal Part of Arabidopsis Phytochrome A Induces Constitutive Photomorphogenic Response显示文摘Phytochrome A (phyA ) 是在 Arabidopsis thaliana 察觉到的 far-red 光的主导的光敏电阻器。phyA 在发信号的变白的幼苗,和导致光的 phyA 的细胞质在高水平积累被一个复杂规章的网络调停。这包括光 -- 并且进在 vivo 的原子核的本国的 phyA 的 FHY1/FHL 蛋白质依赖者 translocation。phyA (PHYA406 ) 的短 N 终端碎片对 phenocopy 足够,这也被显示出在 vitro 的这个高度调整的细胞的过程。为了测试这 N 终端的生物活动,在 planta phyA 碎裂,我们生产了表示 PHYA406YFP 的转基因的 phyA-201 植物(黄荧光灯蛋白质) DD, PHYA406YFPDDNLS (原子本地化信号) ,和 PHYA406YFPDDNES (原子出口信号) 熔化蛋白质。这里,我们报导 PHYA406YFPDD 被进口进原子核,而 PHYA406YFPDDNLS 和 PHYA406YFPDDNES 显示期望的组成的本地化模式,这进程部分是轻依赖者的。我们的结果证明这些截断的 phyA 蛋白质是轻马厩的,当局部性时,他们在他们的原子核,和两个都不触发组成的象 photomorphogenic 一样回答导致合适的 phyA 发信号。我们证明在 vitro 并且在 vivo PHYA406, Pfr 和 Pr 绑 COP1, photomorphogenesis 的一个一般抑压者,并且在原子身体与它共同本地化。因此,我们断定在 planta,截断的 PHYA406 蛋白质以一种光无关的方式在原子核使 COP1 失去活性。 | Andra's Viczia'n E'va Ada'm Iris Wolf Ja'nos Bindics Stefan Kircher Marc Heijde Roman Ulm Eberhard Scha'fer Ferenc Nagy | 2012 | Molecular Plant2012，5，3： | 2 |
| 5 | Deep freezing of concentrated boar semen for intrauterine insemination : effects on sperm viability 显示文摘 | Saravia F Wallgren M Nagy S | 2005 | Theriogenology2005，63，5： | 1 |
| 6 | N-cadherin expressi on in breast cancer:correlati on with an aggressive histologic variant-invasive micropap illary carcinoma显示文摘 | Nagi C Guttman M Jaffer S | 2005 | Breast Cancer Res Treat2005，94，3： | 1 |
| 7 | Treatment of pituitary tumors显示文摘 | Gabriella Iván Nikoletta Szigeti-Csúcs Márk Oláh Cy?rgy M. Nagy Miklós I. Góth | 2005 | Endocrine2005，，1： | 1 |
| 8 | Aluminous cements containing magnesium aluminate spinel from Egyptian dolomite 显示文摘 | NAGY M A EL-HEMALY S A S LAMEY G G | 2001 | Ceram Int2001，27，： | 1 |
| 9 | Clinical significance of subchorionic and retroplacental hematomas detected in the first trimester of pregnancy 显示文摘 | Nagy S Bush M Stone J | 2005 | Orv Hetil2005，146，： | 1 |
| 10 | Ligand binding and activation of the Ah reeeptor显示文摘 | DENISON M PANDINI A NAGY S | 2002 | Chemico-Biological Interactions2002，141，12： | 1 |
| 11 | The value of MALDI-TOFMS for the identification of clinically relevant anaerobic bacteria inroutine laboratories显示文摘 | Nagy E Becker S Kostrzewa M | 2012 | J Med Microbiol2012，61，10： | 1 |
| 12 | Natural mutations lead to enhanced proteasomal degradation of human Ncb5or, a novel flavoheme reductase显示文摘 | Fanni S. Kálmán Beáta Lizák Szilvia K. Nagy Tamás Mészáros Veronika Zámbó József Mandl Miklós Csala éva Kereszturi | 2013 | Biochimie2013，，7： | 1 |
| 13 | Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemicals显示文摘 | Denison M S Nagy S R | 2003 | Annual Review of Pharmacology and Toxicology2003，43，5： | 1 |
| 14 | Lactoperoxidase catalyzed oxidation of thiocyanate by hydrogen peroxide: a reinvestigation of hypothiocyanite by nuclear magnetic resonance and optical spectroscopy 显示文摘 | Nagy P Alguindigue S S Ashby M T | 2006 | Biochemistry2006，45，12： | 1 |
| 15 | Genome sequence and analysis of the tuber crop potato显示文摘 | Xu X Pan S Cheng S Zhang B Mu D Ni P Zhang G Yang S Li R Wang J Orjeda G Guzman F Tones M Lnzano R Ponce O Martinez D De la Cruz G Chakrabarti S K Patil V U Skryabin K G Kuznetsov B B Ravin N V Kolganova T V Beletsky A V Mardanov A V Di Genova A Bolser D M Martin D M Li G Yang Y Kuang H Hu Q Xiong X Bishop G J Sagredo B Mejia N Zagorski W Gromadka R Gawor J Szczesny P Huang S Zhang Z Liang C He J Li Y He Y Xu J Zhang Y Xie B Du Y Qu D Bonierbale M Ghislain M Herrera Mdel R Giuliano G Pietrella M Perrotta G Faeella P O 'Brien K Feingold S E Barreiro L E Massa G A Diambra L Whitty B R Vaillancourt B Lin H Massa A N Geoffroy M Lundback S DellaPenna D Buell C R Sharma S K Marshall D F Waugh R Bryan G J Destefanis M Nagy I Milbourne D Thomson S J Fiefs M Jacobs J M Nielsen K L Sonderkaer M Iovene M Tortes G A Jiang J Veilleux R E Bachem C W de Boer J Bonn T Kloosterman B van Eck H Datema E Hekkert B Goverse A van Ham R C Visser R G | 2011 | Nature2011，475，7355： | 1 |
| 16 | N-cadherin expression in breast cancer: correlation with an aggressive histologic variantinvasive micropapillary carcinoma 显示文摘 | Nagi C Guttman M Jaffer S | 2005 | Breast Cancer Res Treat2005，4，3： | 1 |
| 17 | Zironium oxide/sulfate catalyzed nitration of aromatic compounds 显示文摘 | Nagi S M Zubkov E A Shubin V G | 1990 | Izv Akad Nauk SSSr Ser Khim1990，，7： | 1 |
| 18 | Analysis of risk factors-especially different types of plexitis-for postoperative relapse in Crohn's disease显示文摘AIM To evaluate the presence of submucosal and myenteric plexitis and its role in predicting postoperative recurrence.METHODS Data from all patients who underwent Crohn's disease(CD)-related resection at the University of Szeged, Hungary between 2004 and 2014 were analyzed retrospectively. Demographic data, smoking habits, previous resection, treatment before and after surgery, resection margins, neural fiber hyperplasia, submucosal and myenteric plexitis were evaluated as possible predictors of postoperative recurrence. Histological samples were analyzed blinded to the postoperative outcome and the clinical history of the patient. Plexitis was evaluated based on the appearance of the most severely inflamed ganglion or nerve bundle. Patients underwent regular follow-up with colonoscopy after surgery. Postoperativerecurrence was defined on the basis of endoscopic and clinical findings, and/or the need for additional surgical resection. RESULTS One hundred and four patients were enrolled in the study. Ileocecal, colonic, and small bowel resection were performed in 73.1%, 22.1% and 4.8% of the cases, respectively. Mean disease duration at the time of surgery was 6.25 years. Twenty-six patients underwent previous CD-related surgery. Forty-three point two percent of the patients were on 5-aminosalicylate, 20% on corticosteroid, 68.3% on immunomodulant, and 4% on anti-tumor necrosis factor-alpha postoperative treatment. Postoperative recurrence occurred in 61.5% of the patients; of them 39.1% had surgical recurrence. 92.2% of the recurrences developed within the first five years after the index surgery. Mean disease duration for endoscopic relapse was 2.19 years. The severity of submucosal plexitis was a predictor of the need for second surgery(OR = 1.267, 95%CI: 1.000-1.606, P = 0.050). Female gender(OR = 2.21, 95%CI: 0.98-5.00, P = 0.056), stricturing disease behavior(OR = 3.584, 95%CI: 1.344-9.559, P = 0.011), and isolated ileal localization(OR = 2.671, 95%CI: 1.033-6.910, P = 0.043) were also predictors of postoperative recurrence. No association was revealed between postoperative recurrence and smoking status, postoperative prophylactic treatment and the presence of myenteric plexitis and relapse.CONCLUSION The presence of severe submucosal plexitis with lymphocytes in the proximal resection margin is more likely to result in postoperative relapse in CD. | Agnes Milassin Anita Sejben László Tiszlavicz Zita Reisz Gyorgy Lázár Mónika Szucs Renáta Bor Anita Bálint Mariann Rutka Zoltán Szepes Ferenc Nagy Klaudia Farkas Tamás Molnár | 2017 | World Journal of Gastrointestinal Surgery2017，9，7： | 1 |
| 19 | A prototype document image analysis system for technical journals显示文摘 | S Seth M Viswanathan | 1992 | IEEE Computer1992，25，7： | 1 |
| 20 | N-cadherin expression in breast cancer: correlation with an aggressive histologic variant-invasive micropapillary carcinoma 显示文摘 | Nagi C Guttman M Jaffer S | 2005 | Breast Cancer Res Treat2005，94，3： | 1 |
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