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| 1 | Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter显示文摘As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5'-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity. | CHE NanYing,YANG Yang,LI YanDong,WANG LiLi,HUANG Ping,GAO Yin & An ChengCai The National Laboratory of Protein Engineering and Plant Genetic Engineering,School of Life Sciences,Peking University,Beijing 100871,China | 2009 | Science China(Life Sciences)2009，52，9： | 4 |
| 2 | Progress and Prospect of Studies on Landscape Health of Urban Wetland Park显示文摘Rapid urbanization and increasing attention on the construction of wetland park have contributed to the increasing research fruits of urban wedand park.This paper,on the basis of sorting out literatures on urban wedand park from 2004 to 2015,analyzed the research contents and proposed the prospects.The research contents focused on concept of urban wedand park and landscape health,influence factors,ecological health,landscape health,and evaluation standard.It was found that the research methods vaned,quantitative and qualitative researches were combined;there were rich data sources,dynamic and static researches were integrated;theories lagged behind practices,and the research scale was limited.Therefore,the theoretical system must be improved from the perspective of philosophy and management science;national,regional and other large-scale analysis must be valued to expand the research scale;influence factors were analyzed from multiple perspectives. | YANG Li LI Nani | 2016 | Journal of Landscape Research2016，8，4： | 2 |
| 3 | Space Debris Research Progress of China显示文摘The rapid increase of space debris population has posed serious threaten to the safety of human space activities and became a global issue.How to enhance the technical capabilities of space debris threat coping ability is of great significance to the sustainable development of space activities,the further development,and utilization of outer space.In this paper,we describe space debris research progress of China on observation,collision avoidance,protection,mitigation,regulation,and standard during the last twenty years,and look forward to the future development direction of space debris. | LIU Jing JIANG Hai YANG Xu LI Fen ZHAO Nanying | 2020 | 空间科学学报2020，40，5： | 1 |
| 4 | Origami meets electrospinning:a new strategy for 3D nanofiber scaffolds显示文摘Inspired by the constitution of things in the natural world,three-dimensional (3D)nanofiber scaffold/cells complex was constructed via the combination of electrospinning technology and origami techniques.The nanofiber boxes prepared by origami provided a limited space for the layer-by-layer nanofiber films,and the human fetal osteoblasts (hFOBs)seeded on the both sides of the nanofiber films were expected to facilitate the bonding,of the adjacent nanofiber films through the secretion of extracellular matrix.Specifically,the hFOBs presented 3D distribution in the nanofiber scaffold,and they can stretch across the gaps between the adjacent nanofiber films,forming the cell layers and filling the whole 3D nanofiber scaffold.Eventually,a 3D block composed of electrospun nanofiber scaffold and cells was obtained',which possesses potential applications in bone tissue engineering.Interestingly,we also created 3D nanofiber structures that range from simple forms to intricate architectures via origami,indicating that the combination of electrospinning technology and origami techniques is a feasible method for the 3D construction of tissue engineering scaffolds. | Juqing Song Guanglin Zhu Huichang Gao Lin Wang Nanying Li Xuetao Shi Yingjun Wang | 2018 | Bio-Design and Manufacturing2018，1，4： | 1 |
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