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| 1 | Isolation and diagnosis of Helicobacter pylori by a new method: Microcapillary culture显示文摘AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and histopathologic examination(HE) were performed with biopsy samples.Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 ℃ without providing a microaerophilic environment.Additionally,three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium(Becton Dickinson,Helicobacter Agar,Modified) specific for the isolation of H.pylori and incubated at 37 ℃ in a microaerophilic atmosphere provided by Campy Gen(Becton Dickinson,Gas Pack).Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content.Results obtained by CC,CLO test,and HE were compared with those of MC.The diagnostic performances of the methods used in this study were evaluated for specificity,sensitivity,positive predictive value(PPV),negative predictive value(NPV),and CI.RESULTS:H.pylori was found positive by CLO test +HE and/or CC culture in 26 patient antrum and corpus biopsy samples.In 25(25/26) patient biopsy samples,H.pylori was isolated by MCM,whereas in only 14(14/26) patient biopsy samples,H.pylori was isolatedby CC.CLO test and HE were found positive in 17(17/26) patient biopsy samples.Comparing the results of the isolation of H.pylori by MCM,CC,CLO test,and HE,the sensitivity of the MCM was found as 96%,the specificity as 80%,the PPV as 83%,the NPV as 95%,and the 95%CI as 0.76(χ2 =31.51,P < 0.01) whereas the sensitivity of the CC was found as 54%(χ2 =19.15,P < 0.01),and the sensitivity of the CLO test and HE were found as 65%(χ2 =25.26,P < 0.01).CONCLUSION:This new microcapillary cultivation method for H.pylori has high diagnostic sensitivity compared with CC,HE,and CLO tests. | Adil M Allahverdiyev Melahat Bagirova Reyhan Caliskan Hrisi Bahar Tokman Hayat Aliyeva Gokce Unal Olga Nehir Oztel Emrah Sefik Abamor Hilal Toptas Pelin Yuksel Fatma Kalayci Mustafa Aslan Yusuf Erzin Kadir Bal Bekir S Kocazeybek | 2015 | World Journal of Gastroenterology2015，21，9： | 2 |
| 2 | Geraniol 10-hydroxylase 1 1 The nucleotide sequence newly reported in this paper has been deposited in the GenBank/EMBL data banks with the accession number AJ251269 , a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis显示文摘 | Graziella Collu Nehir Unver Anja M.G. Peltenburg-Looman Robert van der Heijden Robert Verpoorte Johan Memelink | 2001 | FEBS Letters2001，，2： | 2 |
| 3 | The wear of aluminium-basedjournal bearing materials under lubrication显示文摘 | Erol Feyzallahoglu Nehir Sakiroglu | 2010 | Marerials and De-sign2010，31，： | 1 |
| 4 | Prognostic value of initial antithrombin In neonatal sepsis显示文摘 | ERSOY B NEHIR H ALTINOZ S | 2007 | Indian Pediatrics2007，44，8： | 1 |
| 5 | Detection rate of trisomy 21 in fetuses with isolated and non-isolated aberrant right subclavian artery显示文摘 | Esmer AC Gul A Nehir A | 2013 | Fetal Diagn Ther2013，34，3： | 1 |
| 6 | Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis 显示文摘 | Graziella Collu Nehir Unver Anja M G | 2001 | FEBS2001，508，： | 1 |
| 7 | A new approach for development of vaccine against visceral leishmaniasis: Lipophosphoglycan and polyacrylic acid conjugates显示文摘Objective: To determine the antileishmanial vaccine effectiveness of lipophosphoglycan(LPG) and polyacrylic acids(PAA) conjugates on in vivo mice models.Methods: LPG molecule was isolated and purified from large-scale Leishmania donovani parasite culture. Protection efficacies of LPG alone, in combination with Freund's adjuvant, in a physical mixture and in conjugate(consisting of various LPG concentrations) with PAA, were comparatively determined by various techniques, such as cultivation with the micro-culture method, assessment of in vitro infection rates of peritoneal macrophages, determination of parasite load in liver with Leishman-Donovan Units, and detection of cytokine responses.Results: Obtained results demonstrated that the highest vaccine-mediated immune protection was provided by LPG-PAA conjugate due to all parameters investigated. According to the Leishman-Donovan Units results, the sharpest decline in parasite load was seen with a ratio of 81.17% when 35 mg LPG containing conjugate was applied. This value was 44.93% for the control group immunized only with LPG. Moreover, decreases in parasite load were 53.37%, 55.2% and 65.8% for the groups immunized with 10 mg LPG containing LPG-PAA conjugate, a physical mixture of the LPG–PAA, and a mixture of LPG + Freund's adjuvant, respectively. Furthermore, cytokine results supported that Th1 mediated protection occurred when mice were immunized with LPG-PAA conjugate.Conclusions: It has been demonstrated in this study that conjugate of LPG and PAA has an antileishmanial vaccine effect against visceral leishmaniasis. In this respect, the present study may lead to new vaccine approaches based on high immunogenic LPG molecule and adjuvant polymers in fighting against Leishmania infection. | Adil M.Allahverdiyev Rabia Cakir Koc Melahat Bagirova Serhat Elcicek Serap Yesilkir Baydar Olga Nehir Oztel Emrah Sefik Abamor Sezen Canim Ates Murat Topuzogullari Sevil Isoglu Dincer Zeynep Akdeste | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，9： | 1 |
| 8 | Synthesis and antimicrobial activity of some new hydrazones of 4-fluorobenzoic acid hydrazide and 3-acetyl-2, 5-disubstituted-l, 3, 4- oxadiazolines 显示文摘 | Sevim R Nehir G Habibe E | 2002 | Il Farmaco2002，57，： | 1 |
| 9 | Glutathione S-transferase gene polymorphism as a susceptibility factor in smoking-related coronary artery disease显示文摘 | Lülüfer Tamer PhD Bahad?r Ercan Msc Ahmet Camsar? M. D. Hatice Y?ld?r?m Msc Dilek ?i?ek M. D. Nehir Sucu M. D. Nurcan Aras Ate? PhD U?ur Atik PhD | 2004 | Basic Research in Cardiology2004，，3： | 1 |
| 10 | Detection rate of trisomy 21 in fetuses with isolated and non-isolated aberrant right subclavian artery 显示文摘 | Esmer AC Gul A Nehir A | 2013 | Fetal Diagn Ther2013，34，3： | 1 |
| 11 | Synthesis and antimicrobial activity of some new hydrazones of 4-fluorobenzoic acid hydrazide and 3-acetyl-2,5-disubstituted-1,3,4-oxadiazolines显示文摘 | Sevim R Nehir G Habibe E | 2002 | Il Farmaco2002，57，： | 1 |
| 12 | Cervical priming before diagnostic operative hysteroscopy in infertile women: a random- ized, double-blind, controlled comparison of 2 vaginal misopros- tol doses显示文摘 | BASTU E CELIK C NEHIR A | 2013 | Int Surg2013，98，2： | 1 |
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